Closed loop adaptive optics with a laser guide star for biological light microscopy

Saunter, Christopher D.; Bourgenot, Cyril; Girkin, John M.; Love, Gordon D.

Abstract

We report on the development of a widefield microscope that achieves adaptive optics correction through the use of a wavefront sensor observing an artificial laser guide star induced within the sample. By generating this guide star at arbitrary positions and depths within the sample we allow the delivery of high-resolution images. This approach delivers much faster AO correction than image optimization techniques, and allows the use of AO with fluorescent imaging modalities without generating excessive photo-toxic damage in the sample, or inducing significant photo-bleaching in the flurophore molecules.

Citation

Saunter, C. D., Bourgenot, C., Girkin, J. M., & Love, G. D. (2012, January). Closed loop adaptive optics with a laser guide star for biological light microscopy. Presented at MEMS Adaptive Optics VI, San Francisco, CA

• Presentation Conference Type: Conference Paper (published)
• Conference Name: MEMS Adaptive Optics VI
• Start Date: Jan 21, 2012
• End Date: Jan 26, 2012
• Online Publication Date: Feb 15, 2012
• Publication Date: 2012
• Deposit Date: Feb 13, 2018
• Publicly Available Date: Apr 22, 2022
• Volume: 8253
• Series ISSN: 0277-786X
• DOI: https://doi.org/10.1117/12.909927
• Public URL: https://durham-repository.worktribe.com/output/1145835