Outputs (25)

Repo-Man/PP1 regulates heterochromatin formation in interphase (2017)
Journal Article
Castro, I., Budzak, J., Di Giacinto, M. L., Ligammari, L., Gokhan, E., Spanos, C., …Vagnarelli, P. (2017). Repo-Man/PP1 regulates heterochromatin formation in interphase. Nature Communications, 8, Article 14048. https://doi.org/10.1038/ncomms14048

Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Rep... Read More about Repo-Man/PP1 regulates heterochromatin formation in interphase.

A novel function for the MAP kinase SMA-5 in intestinal tube stability (2016)
Journal Article
Geisler, F., Gerhardus, H., Carberry, K., Davis, W., Jorgensen, E., Richardson, C., …Leube, R. E. (2016). A novel function for the MAP kinase SMA-5 in intestinal tube stability. Molecular Biology of the Cell, 27(24), 3855-3868. https://doi.org/10.1091/mbc.e16-02-0099

Intermediate filaments are major cytoskeletal components whose assembly into complex networks and isotype-specific functions are still largely unknown. Caenorhabditis elegans provides an excellent model system to study intermediate filament organizat... Read More about A novel function for the MAP kinase SMA-5 in intestinal tube stability.

Immunoelectron Microscopy of Cryofixed Freeze-Substituted Yeast (2016)
Book Chapter
Fišerová, J., Richardson, C., & Goldberg, M. W. (2016). Immunoelectron Microscopy of Cryofixed Freeze-Substituted Yeast. In S. D. Schwartzbach, O. Skalli, & T. Schikorski (Eds.), High-Resolution Imaging of Cellular Proteins (243-258). Humana Press. https://doi.org/10.1007/978-1-4939-6352-2_15

Immunolabeling electron microscopy is a challenging technique with demands for perfect ultrastructural and antigen preservation. High-pressure freezing offers an excellent way to fix cellular structure. However, its use for immunolabeling has remaine... Read More about Immunoelectron Microscopy of Cryofixed Freeze-Substituted Yeast.

Arabidopsis NAP1 regulates the formation of autophagosomes (2016)
Journal Article
Wang, P., Richardson, C., Hawes, C., & Hussey, P. (2016). Arabidopsis NAP1 regulates the formation of autophagosomes. Current Biology, 26(15), 2060-2069. https://doi.org/10.1016/j.cub.2016.06.008

The SCAR/WAVE complex is required for ARP2/3-mediated actin nucleation, and these complexes are highly conserved in plants and animals [1 and 2]. Proteins from the SCAR/WAVE complex have been found to be membrane associated in plants [3]. Using fluor... Read More about Arabidopsis NAP1 regulates the formation of autophagosomes.

Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development (2016)
Journal Article
Wang, P., Richardson, C., Hawkins, T. J., Sparkes, I., Hawes, C., & Hussey, P. J. (2016). Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development. New Phytologist, 210(4), 1311-1326. https://doi.org/10.1111/nph.13857

The endoplasmic reticulum (ER) is connected to the plasma membrane (PM) through the plant-specific NETWORKED protein, NET3C, and phylogenetically conserved vesicle-associated membrane protein-associated proteins (VAPs). Ten VAP homologues (VAP27-1 to... Read More about Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development.

The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells (2015)
Journal Article
Liu, H., Schneider, H., Recino, A., Richardson, C., Goldberg, M. W., & Rudd, C. E. (2015). The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells. Molecular Cell, 59(5), 840-849. https://doi.org/10.1016/j.molcel.2015.07.015

While immune cell adaptors regulate proximal T cell signaling, direct regulation of the nuclear pore complex (NPC) has not been reported. NPC has cytoplasmic filaments composed of RanGAP1 and RanBP2 with the potential to interact with cytoplasmic med... Read More about The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells.

The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum (2014)
Journal Article
Wang, P., Hawkins, T. J., Richardson, C., Cummins, I., Deeks, M. J., Sparkes, I., …Hussey, P. J. (2014). The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum. Current Biology, 24(12), 1397-1405. https://doi.org/10.1016/j.cub.2014.05.003

The cortical endoplasmic reticulum (ER) network in plants is a highly dynamic structure, and it contacts the plasma membrane (PM) at ER-PM anchor/contact sites. These sites are known to be essential for communication between the ER and PM for lipid t... Read More about The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum.

Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM (2014)
Book Chapter
Kiseleva, E., Richardson, A. C., Fiserova, J., Strunov, A. A., Spink, M. C., Johnson, S. R., & Goldberg, M. W. (2014). Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM. In Nuclear Pore Complexes and Nucleocytoplasmic Transport - Methods (59-79). Elsevier/Academic Press. https://doi.org/10.1016/b978-0-12-417160-2.00003-5

Electron microscopy (EM) has been used extensively for the study of nuclear transport as well as the structure of the nuclear pore complex (NPC) and nuclear envelope. However, there are specific challenges faced when carrying out EM in one of the mai... Read More about Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM.

Nesprin interchain associations control nuclear size. (2012)
Journal Article
Lu, W., Schneider, M., Neumann, S., Jaeger, V., Taranum, S., Munck, M., …Karakesisoglou, I. (2012). Nesprin interchain associations control nuclear size. Cellular and Molecular Life Sciences, 69(20), 3493-3509. https://doi.org/10.1007/s00018-012-1034-1

Nesprins-1/-2/-3/-4 are nuclear envelope proteins, which connect nuclei to the cytoskeleton. The largest nesprin-1/-2 isoforms (termed giant) tether F-actin through their N-terminal actin binding domain (ABD). Nesprin-3, however, lacks an ABD and ass... Read More about Nesprin interchain associations control nuclear size..

A Superfamily of Actin-Binding Proteins at the Actin-Membrane Nexus of Higher Plants. (2012)
Journal Article
Deeks, M. J., Calcutt, J. R., Ingle, E. K., Hawkins, T. J., Chapman, S., Richardson, A., …Hussey, P. J. (2012). at the Actin-Membrane Nexus of Higher Plants. Current Biology, 22(17), 1595-1600. https://doi.org/10.1016/j.cub.2012.06.041

Complex animals use a wide variety of adaptor proteins to produce specialized sites of interaction between actin and membranes. Plants do not have these protein families, yet actin-membrane interactions within plant cells are critical for the positio... Read More about A Superfamily of Actin-Binding Proteins at the Actin-Membrane Nexus of Higher Plants..