Outputs (16)

Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization (2020)
Journal Article
Kalligeraki, A. A., Isted, A., Pal, R., Saunter, C., Girkin, J., Jarrin, M., Uwineza, A., Obara, B., & Quinlan, R. A. (2020). Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization. Scientific Reports, 10, Article 16898. https://doi.org/10.1038/s41598-020-73625-9

Organ and tissue development are highly coordinated processes; lens growth and functional integration into the eye (emmetropia) is a robust example. An epithelial monolayer covers the anterior hemisphere of the lens, and its organization is the key t... Read More about Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization.

Design, manufacture, and evaluation of prototype telescope windows for use in low-vision aids (2017)
Presentation / Conference Contribution
Young, L., Robertson, D., Love, G., Girkin, J., Cowie, E., Bourgenot, C., & Courtial, J. (2017, November). Design, manufacture, and evaluation of prototype telescope windows for use in low-vision aids. Presented at Novel Optical Systems Design and Optimization XX, San Diego, California

Pixellated Optics, a class of optical devices which preserve phase front continuity only over small sub areas of the device, allow for a range of uses that would not otherwise be possible. One potential use is as Low Vision Aids (LVAs), where they ar... Read More about Design, manufacture, and evaluation of prototype telescope windows for use in low-vision aids.

Realtime wavefront sensing in a SPIM microscope, and active aberration tracking (2015)
Presentation / Conference Contribution
Taylor, J. M., Saunter, C. D., Bourgenot, C., Girkin, J. M., & Love, G. D. (2015, February). Realtime wavefront sensing in a SPIM microscope, and active aberration tracking. Presented at Adaptive Optics and Wavefront Control for Biological Systems, San Francisco, CA

Adaptive optics (AO) can potentially allow high resolution imaging deep inside living tissue, mitigating against the loss of resolution due to aberrations caused by overlying tissue. Closed-loop AO correction is particularly attractive for moving tis... Read More about Realtime wavefront sensing in a SPIM microscope, and active aberration tracking.

Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy (2014)
Journal Article
Mitchell, T., Saunter, C., O'Nions, W., Girkin, J., & Love, G. (2014). Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy. Review of Scientific Instruments, 85(10), Article 103713. https://doi.org/10.1063/1.4899208

Modern developmental biology relies on optically-sectioning uorescence microscope techniques to produce non-destructive in-vivo images of developing specimens at high resolution in three dimensions. As optimal performance of these techniques is relia... Read More about Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy.

AO modal optimization in a live, beating zebrafish heart (2013)
Presentation / Conference Contribution
Bourgenot, C., Taylor, J. M., Saunter, C. D., Girkin, J. M., & Love, G. D. (2013, June). AO modal optimization in a live, beating zebrafish heart. Presented at Imaging and Applied Optics Postdeadline, Arlington, Virginia, United States

We report on the combinated use of heart synchronization and wavefront sensorless modal AO to enable significant improvement in image quality of a live transgenic zebrafish embryo heart, imaged with a light sheet microscope.

Laser-targeted ablation of the zebrafish embryonic ventricle: A novel model of cardiac injury and repair (2013)
Journal Article
Matrone, G., Taylor, J. M., Wilson, K. S., Baily, J., Love, G. D., Girkin, J. M., …Denvir, M. A. (2013). Laser-targeted ablation of the zebrafish embryonic ventricle: A novel model of cardiac injury and repair. International Journal of Cardiology, 168(4), 3913-3919. https://doi.org/10.1016/j.ijcard.2013.06.063

Background: While the adult zebrafish (Danio rerio) heart demonstrates a remarkable capacity for self-renewal following apical resection little is known about the response to injury in the embryonic heart. Methods: Injury to the beating zebrafish emb... Read More about Laser-targeted ablation of the zebrafish embryonic ventricle: A novel model of cardiac injury and repair.

Comparison of closed loop and sensorless adaptive optics in widefield optical microscopy (2013)
Journal Article
Bourgenot, C., Saunter, C., Love, G., & Girkin, J. (2013). Comparison of closed loop and sensorless adaptive optics in widefield optical microscopy. Journal of the European Optical Society-Rapid Publications, 8, Article 13027. https://doi.org/10.2971/jeos.2013.13027

We report on a closed loop widefield adaptive optics, optical microscopy system in which the feedback signal is provided by backscattered light from the sample acting as a guide star. The improvement in imaging performance is compared to an adaptive... Read More about Comparison of closed loop and sensorless adaptive optics in widefield optical microscopy.

Light sheet adaptive optics microscope for 3D live imaging (2013)
Presentation / Conference Contribution
Bourgenot, C., Taylor, J., Saunter, C., Girkin, J., & Love, G. (2013, February). Light sheet adaptive optics microscope for 3D live imaging. Presented at Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, San Francisco, California, USA

We report on the incorporation of adaptive optics (AO) into the imaging arm of a selective plane illumination microscope (SPIM). SPIM has recently emerged as an important tool for life science research due to its ability to deliver high-speed, optica... Read More about Light sheet adaptive optics microscope for 3D live imaging.

Adaptive optical methods for in vivo imaging in developing Zebra fish (2012)
Presentation / Conference Contribution
Girkin, J., Taylor, J., Bourgenot, C., Saunter, C., & Love, G. (2012, December). Adaptive optical methods for in vivo imaging in developing Zebra fish. Presented at 2012 International Symposium on Optomechatronic Technologies (ISOT 2012), Paris

The humble Zebra fish is rapidly establishing itself as the model of choice for a wide range of biological investigations, in particular at the developing embryo stage. Single Plane Illumination Microscopy (SPIM) has already been shown to be a very p... Read More about Adaptive optical methods for in vivo imaging in developing Zebra fish.

3D adaptive optics in a light sheet microscope (2012)
Journal Article
Bourgenot, C., Saunter, C. D., Taylor, J. M., Girkin, J. M., & Love, G. D. (2012). 3D adaptive optics in a light sheet microscope. Optics Express, 20(12), 13252-13261. https://doi.org/10.1364/oe.20.013252

We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavef... Read More about 3D adaptive optics in a light sheet microscope.