Outputs (15)

Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization (2020)
Journal Article
Kalligeraki, A. A., Isted, A., Pal, R., Saunter, C., Girkin, J., Jarrin, M., …Quinlan, R. A. (2020). Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization. Scientific Reports, 10, Article 16898. https://doi.org/10.1038/s41598-020-73625-9

Organ and tissue development are highly coordinated processes; lens growth and functional integration into the eye (emmetropia) is a robust example. An epithelial monolayer covers the anterior hemisphere of the lens, and its organization is the key t... Read More about Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization.

Generating artificial reference images for open loop correlation wavefront sensors (2018)
Journal Article
Townson, M., Love, G., & Saunter, C. (2018). Generating artificial reference images for open loop correlation wavefront sensors. Monthly Notices of the Royal Astronomical Society, 479(2), 1595-1602. https://doi.org/10.1093/mnras/sty1362

Shack–Hartmann wavefront sensors for both solar and laser guide star adaptive optics (with elongated spots) need to observe extended objects. Correlation techniques have been successfully employed to measure the wavefront gradient in solar adaptive o... Read More about Generating artificial reference images for open loop correlation wavefront sensors.

Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy (2014)
Journal Article
Mitchell, T., Saunter, C., O'Nions, W., Girkin, J., & Love, G. (2014). Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy. Review of Scientific Instruments, 85(10), Article 103713. https://doi.org/10.1063/1.4899208

Modern developmental biology relies on optically-sectioning uorescence microscope techniques to produce non-destructive in-vivo images of developing specimens at high resolution in three dimensions. As optimal performance of these techniques is relia... Read More about Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy.

AO modal optimization in a live, beating zebrafish heart (2013)
Presentation / Conference Contribution
Bourgenot, C., Taylor, J. M., Saunter, C. D., Girkin, J. M., & Love, G. D. (2013). AO modal optimization in a live, beating zebrafish heart. . https://doi.org/10.1364/aopt.2013.ow4a.4

We report on the combinated use of heart synchronization and wavefront sensorless modal AO to enable significant improvement in image quality of a live transgenic zebrafish embryo heart, imaged with a light sheet microscope.

Comparison of closed loop and sensorless adaptive optics in widefield optical microscopy (2013)
Journal Article
Bourgenot, C., Saunter, C., Love, G., & Girkin, J. (2013). Comparison of closed loop and sensorless adaptive optics in widefield optical microscopy. Journal of the European Optical Society-Rapid Publications, 8, Article 13027. https://doi.org/10.2971/jeos.2013.13027

We report on a closed loop widefield adaptive optics, optical microscopy system in which the feedback signal is provided by backscattered light from the sample acting as a guide star. The improvement in imaging performance is compared to an adaptive... Read More about Comparison of closed loop and sensorless adaptive optics in widefield optical microscopy.

Light sheet adaptive optics microscope for 3D live imaging (2013)
Presentation / Conference Contribution
Bourgenot, C., Taylor, J., Saunter, C., Girkin, J., & Love, G. (2013). Light sheet adaptive optics microscope for 3D live imaging. In C. J. Cogswell (Ed.), . https://doi.org/10.1117/12.2002057

We report on the incorporation of adaptive optics (AO) into the imaging arm of a selective plane illumination microscope (SPIM). SPIM has recently emerged as an important tool for life science research due to its ability to deliver high-speed, optica... Read More about Light sheet adaptive optics microscope for 3D live imaging.

Adaptive optical methods for in vivo imaging in developing Zebra fish (2012)
Presentation / Conference Contribution
Girkin, J., Taylor, J., Bourgenot, C., Saunter, C., & Love, G. (2012). Adaptive optical methods for in vivo imaging in developing Zebra fish. In 2012 International Symposium on Optomechatronic Technologies (ISOT 2012). https://doi.org/10.1109/isot.2012.6403295

The humble Zebra fish is rapidly establishing itself as the model of choice for a wide range of biological investigations, in particular at the developing embryo stage. Single Plane Illumination Microscopy (SPIM) has already been shown to be a very p... Read More about Adaptive optical methods for in vivo imaging in developing Zebra fish.

3D adaptive optics in a light sheet microscope (2012)
Journal Article
Bourgenot, C., Saunter, C. D., Taylor, J. M., Girkin, J. M., & Love, G. D. (2012). 3D adaptive optics in a light sheet microscope. Optics Express, 20(12), 13252-13261. https://doi.org/10.1364/oe.20.013252

We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavef... Read More about 3D adaptive optics in a light sheet microscope.

Closed loop adaptive optics with a laser guide star for biological light microscopy (2012)
Presentation / Conference Contribution
Saunter, C. D., Bourgenot, C., Girkin, J. M., & Love, G. D. (2012). Closed loop adaptive optics with a laser guide star for biological light microscopy. In S. S. Oliver, T. G. Bifano, & J. Kubby (Eds.), . https://doi.org/10.1117/12.909927

We report on the development of a widefield microscope that achieves adaptive optics correction through the use of a wavefront sensor observing an artificial laser guide star induced within the sample. By generating this guide star at arbitrary posit... Read More about Closed loop adaptive optics with a laser guide star for biological light microscopy.

Wide field adaptive optics microscopy using both closed loop correction and image sharpness optimization (2011)
Presentation / Conference Contribution
Bourgenot, C., Love, G. D., Saunter, C. D., & Girkin, J. M. (2011). Wide field adaptive optics microscopy using both closed loop correction and image sharpness optimization. . https://doi.org/10.1364/aopt.2011.jtua2

We report on results from a wide field microscope fitted with adaptive optics. We describe results based on both image optimization (wavefront sensorless adaptive optics) and full closed loop correction.