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Outputs (173)

VceR regulates the vceCAB drug efflux pump operon of Vibrio cholerae by alternating between mutually exclusive conformations that bind either drugs or promoter DNA. (2005)
Journal Article
Borges-Walmsley, M., Du, D., McKeegan, K., Sharples, G., & Walmsley, A. (2005). VceR regulates the vceCAB drug efflux pump operon of Vibrio cholerae by alternating between mutually exclusive conformations that bind either drugs or promoter DNA. Journal of Molecular Biology, 349(2), 387-400

VceR, a member of the TetR family of transcriptional regulators, is a repressor of the vceCAB operon, which encodes a multidrug efflux pump in Vibrio cholerae. VceR binds to a 28 bp inverted-repeat within the vceR-vceC intergenic region and is dissoc... Read More about VceR regulates the vceCAB drug efflux pump operon of Vibrio cholerae by alternating between mutually exclusive conformations that bind either drugs or promoter DNA..

The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis. (2005)
Journal Article
Sanchez, H., Kidane, D., Reed, P., Curtis, F., Cozar, M., Graumann, P., …Alonso, J. (2005). The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis. Genetics, 171, 873-883

In models of Escherichia coli recombination and DNA repair, the RuvABC complex directs the branch migration and resolution of Holliday junction DNA. To probe the validity of the E. coli paradigm, we examined the impact of mutations in DeltaruvAB and... Read More about The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis..

Conservation of RecG activity from pathogens to hyperthermophiles. (2005)
Journal Article
Wen, Q., Mahdi, A., Briggs, G., Sharples, G., & Lloyd, R. (2005). Conservation of RecG activity from pathogens to hyperthermophiles. DNA Repair, 4, 23-31

Maintaining the integrity of the genome is essential for the survival of all organisms. RecG helicase plays an important part in this process in Escherichia coli, promoting recombination and DNA repair, and providing ways to rescue stalled replicatio... Read More about Conservation of RecG activity from pathogens to hyperthermophiles..

Functional similarities between phage lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange (2005)
Journal Article
Maxwell, K., Reed, P., Zhang, R., Beasley, S., Walmsley, A., Curtis, F., …Sharples, G. (2005). Functional similarities between phage lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange. Proceedings of the National Academy of Sciences, 102(32), 11260-11265. https://doi.org/10.1073/pnas.0503399102

Genetic recombination in bacteriophage lambda relies on DNA end processing by Exo to expose 3'-tailed strands for annealing and exchange by beta protein. Phage lambda encodes an additional recombinase, Orf, which participates in the early stages of r... Read More about Functional similarities between phage lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange.

An evolutionarily conserved coiled-coil protein implicated in polycystic kidney disease is involved in basal body duplication and flagellar biogenesis in Trypanosoma brucei (2005)
Journal Article
Morgan, G., Denny, P., Vaughan, S., Goulding, D., Jeffries, T., Smith, D., …Field, M. (2005). An evolutionarily conserved coiled-coil protein implicated in polycystic kidney disease is involved in basal body duplication and flagellar biogenesis in Trypanosoma brucei. Molecular and Cellular Biology, 25(9), 3774-3783. https://doi.org/10.1128/mcb.25.9.3774-3783.2005

Trypanosoma brucei is a flagellated protozoan with a highly polarized cellular structure. TbLRTP is a trypanosomal protein containing multiple SDS22-class leucine-rich repeats and a coiled-coil domain with high similarity to a mammalian testis-specif... Read More about An evolutionarily conserved coiled-coil protein implicated in polycystic kidney disease is involved in basal body duplication and flagellar biogenesis in Trypanosoma brucei.

The crystal structure of the outer membrane protein VceC from the bacterial pathogen Vibrio cholerae at 1.8 Å resolution (2005)
Journal Article
Federici, L., Du, D., Walas, F., Matsumura, H., Fernandez-Recio, J., McKeegan, K., …Walmsley, A. (2005). The crystal structure of the outer membrane protein VceC from the bacterial pathogen Vibrio cholerae at 1.8 Å resolution. Journal of Biological Chemistry, 280(15), 15307-15314. https://doi.org/10.1074/jbc.m500401200

Multidrug resistance in Gram-negative bacteria arises in part from the activities of tripartite drug efflux pumps. In the pathogen Vibrio cholerae, one such pump comprises the inner membrane proton antiporter VceB, the periplasmic adaptor VceA, and t... Read More about The crystal structure of the outer membrane protein VceC from the bacterial pathogen Vibrio cholerae at 1.8 Å resolution.

Increased Heat Shock Protein 70 Expression Following Toxicant-Mediated Cytotoxicity: A Ubiquitous Marker Of Toxicant Exposure? (2005)
Journal Article
Farzaneh, P., Allameh, A., Pratt, S., Moore, N., Travis, L., Gottschalg, E., …Fry, J. (2005). Increased Heat Shock Protein 70 Expression Following Toxicant-Mediated Cytotoxicity: A Ubiquitous Marker Of Toxicant Exposure?. Alternatives to Laboratory Animals, 33(2), 105-110

The up-regulation of heat shock protein (HSP) expression has been proposed as a general biomarker of cellular protection against various environmental stresses and chemicals. The present study investigated the possibility of using HSP70 up-regulation... Read More about Increased Heat Shock Protein 70 Expression Following Toxicant-Mediated Cytotoxicity: A Ubiquitous Marker Of Toxicant Exposure?.

Evolution of a phage RuvC endonuclease for resolution of both Holliday and branched DNA junctions (2005)
Journal Article
Curtis, F., Reed, P., & Sharples, G. (2005). Evolution of a phage RuvC endonuclease for resolution of both Holliday and branched DNA junctions. Molecular Microbiology, 55(5), 1332-1345. https://doi.org/10.1111/j.1365-2958.2004.04476.x

Resolution of Holliday junction recombination intermediates in most Gram-negative bacteria is accomplished by the RuvC endonuclease acting in concert with the RuvAB branch migration machinery. Gram-positive species, however, lack RuvC, with the excep... Read More about Evolution of a phage RuvC endonuclease for resolution of both Holliday and branched DNA junctions.

Direct transport across the plasma membrane of mammalian cells of Leishmania HASPB as revealed by a CHO export mutant (2005)
Journal Article
Stegmayer, C., Kehlenbach, A., Tournaviti, S., Wegehingel, S., Zehe, C., Denny, P., …Nickel, W. (2005). Direct transport across the plasma membrane of mammalian cells of Leishmania HASPB as revealed by a CHO export mutant. Journal of Cell Science, 118(3), 517-527. https://doi.org/10.1242/jcs.01645

Leishmania HASPB is a lipoprotein that is exported to the extracellular space from both Leishmania parasites and mammalian cells via an unconventional secretory pathway. Exported HASPB remains anchored in the outer leaflet of the plasma membrane medi... Read More about Direct transport across the plasma membrane of mammalian cells of Leishmania HASPB as revealed by a CHO export mutant.

Leishmania major: clathrin and adaptin complexes of an intra-cellular parasite (2005)
Journal Article
Denny, P., Morgan, G., Field, M., & Smith, D. (2005). Leishmania major: clathrin and adaptin complexes of an intra-cellular parasite. Experimental Parasitology, 109(1), 33-37. https://doi.org/10.1016/j.exppara.2004.10.007

To investigate the role of clathrin-mediated trafficking during the Leishmania lifecycle, open reading frames encoding clathrin heavy chain and the β-adaptins, major components of the adaptor complexes, have been analysed both in silico and experimen... Read More about Leishmania major: clathrin and adaptin complexes of an intra-cellular parasite.

Novel antibiotics: second generation macrocyclic peptides designed to trap Holliday junctions. (2004)
Journal Article
Liotta, L., Medina, I., Robinson, J., Carroll, C., Pan, P., Corral, R., …McAlpine, S. (2004). Novel antibiotics: second generation macrocyclic peptides designed to trap Holliday junctions. Tetrahedron Letters, 45, 8447-8450. https://doi.org/10.1016/j.tetlet.2004.09.084

Described are the syntheses of 15 macrocyclic peptides designed to trap Holliday junctions(HJs) in bacteria during site-specific and homologous recombination. This leads to inhibiting bacterial growth. These second generation macrocycles were based o... Read More about Novel antibiotics: second generation macrocyclic peptides designed to trap Holliday junctions..

Rafts and sphingolipid biosynthesis in the kinetoplastid parasitic protozoa (2004)
Journal Article
Denny, P., & Smith, D. (2004). Rafts and sphingolipid biosynthesis in the kinetoplastid parasitic protozoa. Molecular Microbiology, 53(3), 725-733. https://doi.org/10.1111/j.1365-2958.2004.04208.x

Although the concept of eukaryotic lipid rafts arouses controversy, recent biophysical studies strongly indicate that inducible, stable and relatively large rafts are a feature of activated mammalian cells. These studies allow us to consider the func... Read More about Rafts and sphingolipid biosynthesis in the kinetoplastid parasitic protozoa.

Conformational Flexibility in Recombinant Measles Virus Nucleocapsids Visualised by Cryo-negative Stain Electron Microscopy and Real-space Helical Reconstruction (2004)
Journal Article
Yeo, R., Bhella, D., & Ralph, A. (2004). Conformational Flexibility in Recombinant Measles Virus Nucleocapsids Visualised by Cryo-negative Stain Electron Microscopy and Real-space Helical Reconstruction. Journal of Molecular Biology, 340(2), 319-331. https://doi.org/10.1016/j.jmb.2004.05.015

Measles virus is a highly contagious virus that, despite the existence of an effective vaccine, is a major cause of illness and mortality worldwide. The virus has a negative-sense, single-stranded RNA genome that is encapsidated by the nucleocapsid p... Read More about Conformational Flexibility in Recombinant Measles Virus Nucleocapsids Visualised by Cryo-negative Stain Electron Microscopy and Real-space Helical Reconstruction.

Holliday Junction Binding and Resolution by the Rap Structure-specific Endonuclease of Phage lambda (2004)
Journal Article
Sharples, G., Curtis, F., McGlynn, P., & Bolt, E. (2004). Holliday Junction Binding and Resolution by the Rap Structure-specific Endonuclease of Phage lambda. Journal of Molecular Biology, 340(4), 739-751. https://doi.org/10.1016/j.jmb.2004.05.030

Rap endonuclease targets recombinant joint molecules arising from phage lambda Red-mediated genetic exchange. Previous studies revealed that Rap nicks DNA at the branch point of synthetic Holliday junctions and other DNA structures with a branched co... Read More about Holliday Junction Binding and Resolution by the Rap Structure-specific Endonuclease of Phage lambda.

Sphingolipid-free Leishmania are defective in membrane trafficking, differentiation and infectivity (2004)
Journal Article
Denny, P., Goulding, D., Ferguson, M., & Smith, D. (2004). Sphingolipid-free Leishmania are defective in membrane trafficking, differentiation and infectivity. Molecular Microbiology, 52(2), 313-327. https://doi.org/10.1111/j.1365-2958.2003.03975.x

Sphingolipids are structural components of the eukaryotic plasma membrane that are involved, together with cholesterol, in the formation of lipid microdomains (rafts). Additionally, sphingolipid metabolites have been shown to modulate a wide variety... Read More about Sphingolipid-free Leishmania are defective in membrane trafficking, differentiation and infectivity.

A model for dsDNA translocation revealed by a structural motif common to RecG and Mfd proteins. (2003)
Journal Article
Mahdi, A., Briggs, G., Sharples, G., Wen, Q., & Lloyd, R. (2003). A model for dsDNA translocation revealed by a structural motif common to RecG and Mfd proteins. The EMBO Journal, 22, 724-734

RecG protein differs from other helicases analysed to atomic resolution in that it mediates strand separation via translocation on double-stranded (ds) rather than single-stranded (ss) DNA. We describe a highly conserved helical hairpin motif in RecG... Read More about A model for dsDNA translocation revealed by a structural motif common to RecG and Mfd proteins..

The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold. (2003)
Journal Article
Rafferty, J., Bolt, E., Muranova, T., Sedelnikova, S., Leonard, P., Pasquo, A., …Lloyd, R. (2003). The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold. Structure, 11, 1557-1567

Holliday junction resolution performed by a variety of structure-specific endonucleases is a key step in DNA recombination and repair. It is believed that all resolvases carry out their reaction chemistries in a similar fashion, utilizing a divalent... Read More about The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold..

The RdgC protein of Escherichia coli binds DNA and counters a toxic effect of RecFOR in strains lacking the replication restart protein PriA. (2003)
Journal Article
Moore, T., McGlynn, P., Ngo, H., Sharples, G., & Lloyd, R. (2003). The RdgC protein of Escherichia coli binds DNA and counters a toxic effect of RecFOR in strains lacking the replication restart protein PriA. The EMBO Journal, 22, 735-745

PriA protein provides a means to load the DnaB replicative helicase at DNA replication fork and D loop structures, and is therefore a key factor in the rescue of stalled or broken forks and subsequent replication restart. We show that the nucleoid-as... Read More about The RdgC protein of Escherichia coli binds DNA and counters a toxic effect of RecFOR in strains lacking the replication restart protein PriA..