Outputs (83)

Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM (2018)
Journal Article
Young, L., Jarrin, M., Saunter, C., Quinlan, R., & Girkin, J. (2018). Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM. Biomedical Optics Express, 9(5), 2176-2188. https://doi.org/10.1364/boe.9.002176

Graded refractive index lenses are inherent to advanced visual systems in animals. By understanding their formation and local optical properties, significant potential for improved ocular healthcare may be realized. We report a novel technique measur... Read More about Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM.

The light-sheet microscopy revolution (2018)
Journal Article
Girkin, J. M., & Carvalho, M. T. (2018). The light-sheet microscopy revolution. Journal of Optics, 20(5), Article 053002. https://doi.org/10.1088/2040-8986/aab58a

This paper reviews the rapid advances that have been made in one form of optical biological imaging in the last decade, namely that of light-sheet microscopy. Although the concept was originally presented over one hundred years ago, it was a methodol... Read More about The light-sheet microscopy revolution.

Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish (2017)
Journal Article
Buckley, C., Carvalho, M., Young, L., Rider, S., McFadden, C., Berlage, C., …Mullins, J. (2017). Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish. Scientific Reports, 7(1), Article 5096. https://doi.org/10.1038/s41598-017-05028-2

The ability to kill individual or groups of cells in vivo is important for studying cellular processes and their physiological function. Cell-specific genetically encoded photosensitizing proteins, such as KillerRed, permit spatiotemporal optogenetic... Read More about Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish.

Towards a high-throughput real-time confocal microfluidic system for monitoring absorbance spectra in mixed-phase chemical reactions (2017)
Journal Article
Lawton, P., & Girkin, J. (2017). Towards a high-throughput real-time confocal microfluidic system for monitoring absorbance spectra in mixed-phase chemical reactions. Microfluidics and Nanofluidics, 21(4), Article 69. https://doi.org/10.1007/s10404-017-1904-9

In this work, we present a compact, real-time absorbance spectroscopy instrument, designed with a particular focus on taking spectroscopic readings in a microfluidic channel environment and selectively analysing an active volume in this channel by ad... Read More about Towards a high-throughput real-time confocal microfluidic system for monitoring absorbance spectra in mixed-phase chemical reactions.

Advancing Age Decreases Pressure-Sensitive Modulation of Calcium Signaling in the Endothelium of Intact and Pressurized Arteries (2017)
Journal Article
Wilson, C., Saunter, C. D., Girkin, J. M., & McCarron, J. G. (2017). Advancing Age Decreases Pressure-Sensitive Modulation of Calcium Signaling in the Endothelium of Intact and Pressurized Arteries. Journal of Vascular Research, 53(5-6), 358-369. https://doi.org/10.1159/000454811

Aging is the summation of many subtle changes which result in altered cardiovascular function. Impaired endothelial function underlies several of these changes and precipitates plaque development in larger arteries. The endothelium transduces chemica... Read More about Advancing Age Decreases Pressure-Sensitive Modulation of Calcium Signaling in the Endothelium of Intact and Pressurized Arteries.

Shear banding in large amplitude oscillatory shear (LAOStrain and LAOStress) of polymers and wormlike micelles (2016)
Journal Article
Carter, K. A., Girkin, J. M., & Fielding, S. M. (2016). Shear banding in large amplitude oscillatory shear (LAOStrain and LAOStress) of polymers and wormlike micelles. Journal of Rheology, 60(5), Article 883. https://doi.org/10.1122/1.4960512

We investigate theoretically shear banding in large amplitude oscillatory shear (LAOS) of polymers and wormlike micelles. In LAOStrain we find banding at low frequencies and sufficiently high strain rate amplitudes in fluids for which the underlying... Read More about Shear banding in large amplitude oscillatory shear (LAOStrain and LAOStress) of polymers and wormlike micelles.

Two-color widefield fluorescence microendoscopy enables multiplexed molecular imaging in the alveolar space of human lung tissue (2016)
Journal Article
Krstajić, N., Akram, A. R., Choudhary, T. R., McDonald, N., Tanner, M. G., Pedretti, E., …Dhaliwal, K. (2016). Two-color widefield fluorescence microendoscopy enables multiplexed molecular imaging in the alveolar space of human lung tissue. Journal of Biomedical Optics, 21(4), Article 046009. https://doi.org/10.1117/1.jbo.21.4.046009

We demonstrate a fast two-color widefield fluorescence microendoscopy system capable of simultaneously detecting several disease targets in intact human ex vivo lung tissue. We characterize the system for light throughput from the excitation light em... Read More about Two-color widefield fluorescence microendoscopy enables multiplexed molecular imaging in the alveolar space of human lung tissue.

Age decreases mitochondrial motility and increases mitochondrial size in vascular smooth muscle (2016)
Journal Article
Chalmers, S., Saunter, C., Girkin, J., & McCarron, J. (2016). Age decreases mitochondrial motility and increases mitochondrial size in vascular smooth muscle. The Journal of Physiology, 594(15), 4283-4295. https://doi.org/10.1113/jp271942

Mitochondrial function, motility and architecture are each central to cell function. Age-associated mitochondrial dysfunction may contribute to vascular disease. However, mitochondrial changes in ageing remain ill-defined because of the challenges of... Read More about Age decreases mitochondrial motility and increases mitochondrial size in vascular smooth muscle.

Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium (2016)
Journal Article
Wilson, C., Saunter, C. D., Girkin, J. M., & McCarron, J. G. (2016). Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium. FASEB Journal, 30(5), 2000-2013. https://doi.org/10.1096/fj.201500090

Agonist-mediated signaling by the endothelium controls virtually all vascular functions. Because of the large diversity of agonists, each with varying concentrations, background noise often obscures individual cellular signals. How the endothelium di... Read More about Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium.

Flicker-assisted localization microscopy reveals altered mitochondrial architecture in hypertension (2015)
Journal Article
Chalmers, S., Saunter, C. D., Girkin, J. M., & McCarron, J. G. (2015). Flicker-assisted localization microscopy reveals altered mitochondrial architecture in hypertension. Scientific Reports, 5, Article 16875. https://doi.org/10.1038/srep16875

Mitochondrial morphology is central to normal physiology and disease development. However, in many live cells and tissues, complex mitochondrial structures exist and morphology has been difficult to quantify. We have measured the shape of electricall... Read More about Flicker-assisted localization microscopy reveals altered mitochondrial architecture in hypertension.

Pressure-dependent regulation of Ca2+ signalling in the vascular endothelium (2015)
Journal Article
Wilson, C., Saunter, C. D., Girkin, J. M., & McCarron, J. G. (2015). Pressure-dependent regulation of Ca2+ signalling in the vascular endothelium. The Journal of Physiology, 593(24), 5231-5253. https://doi.org/10.1113/jp271157

The endothelium is an interconnected network upon which haemodynamic mechanical forces act to control vascular tone and remodelling in disease. Ca2+ signalling is central to the endothelium's mechanotransduction and networked activity. However, chall... Read More about Pressure-dependent regulation of Ca2+ signalling in the vascular endothelium.

A dimensionless ordered pull-through model of the mammalian lens epithelium evidences scaling across species and explains the age-dependent changes in cell density in the human lens (2015)
Journal Article
Wu, J., Wu, W., Tholozan, F., Saunter, C., Girkin, J., & Quinlan, R. (2015). A dimensionless ordered pull-through model of the mammalian lens epithelium evidences scaling across species and explains the age-dependent changes in cell density in the human lens. Journal of the Royal Society. Interface, 12(108), https://doi.org/10.1098/rsif.2015.0391

We present a mathematical (ordered pull-through; OPT) model of the cell-density profile for the mammalian lens epithelium together with new experimental data. The model is based upon dimensionless parameters, an important criterion for inter-species... Read More about A dimensionless ordered pull-through model of the mammalian lens epithelium evidences scaling across species and explains the age-dependent changes in cell density in the human lens.

Realtime wavefront sensing in a SPIM microscope, and active aberration tracking (2015)
Presentation / Conference Contribution
Taylor, J. M., Saunter, C. D., Bourgenot, C., Girkin, J. M., & Love, G. D. (2015, February). Realtime wavefront sensing in a SPIM microscope, and active aberration tracking. Presented at Adaptive Optics and Wavefront Control for Biological Systems, San Francisco, CA

Adaptive optics (AO) can potentially allow high resolution imaging deep inside living tissue, mitigating against the loss of resolution due to aberrations caused by overlying tissue. Closed-loop AO correction is particularly attractive for moving tis... Read More about Realtime wavefront sensing in a SPIM microscope, and active aberration tracking.

Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy (2014)
Journal Article
Mitchell, T., Saunter, C., O'Nions, W., Girkin, J., & Love, G. (2014). Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy. Review of Scientific Instruments, 85(10), Article 103713. https://doi.org/10.1063/1.4899208

Modern developmental biology relies on optically-sectioning uorescence microscope techniques to produce non-destructive in-vivo images of developing specimens at high resolution in three dimensions. As optimal performance of these techniques is relia... Read More about Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy.

Enamel erosion and prevention efficacy characterized by confocal laser scanning microscope (2014)
Journal Article
Maia, A., Longbottom, C., Gomes, A., & Girkin, J. (2014). Enamel erosion and prevention efficacy characterized by confocal laser scanning microscope. Microscopy Research and Technique, 77(6), 439-445. https://doi.org/10.1002/jemt.22364

The aim of this study was to evaluate the erosion-inhibiting effect of two toothpastes on the development of erosion-like lesions, by a confocal laser scanning microscope (CLSM). Forty human enamel blocks were divided into five groups (n = 8), in acc... Read More about Enamel erosion and prevention efficacy characterized by confocal laser scanning microscope.

Combining optical trapping in a microfluidic channel with simultaneous micro-Raman spectroscopy and motion detection (2014)
Presentation / Conference Contribution
Lawton, P., Saunter, C., & Girkin, J. (2014, March). Combining optical trapping in a microfluidic channel with simultaneous micro-Raman spectroscopy and motion detection. Presented at Microfluidics, BioMEMS, and Medical Microsystems XII, San Francisco, California, United States

Since their invention by Ashkin optical tweezers have demonstrated their ability and versatility as a non-invasive tool for micromanipulation. One of the most useful additions to the basic optical tweezers system is micro-Raman spectroscopy, which pe... Read More about Combining optical trapping in a microfluidic channel with simultaneous micro-Raman spectroscopy and motion detection.

Investigating the micro-rheology of the vitreous humor using an optically trapped local probe (2014)
Journal Article
Watts, F., Tan, L. E., Wilson, C. G., Girkin, J. M., Tassieri, M., & Wright, A. J. (2014). Investigating the micro-rheology of the vitreous humor using an optically trapped local probe. Journal of Optics, 16(1), https://doi.org/10.1088/2040-8978/16/1/015301

We demonstrate that an optically trapped silica bead can be used as a local probe to measure the micro-rheology of the vitreous humor. The Brownian motion of the bead was observed using a fast camera and the micro-rheology determined by analysis of t... Read More about Investigating the micro-rheology of the vitreous humor using an optically trapped local probe.

Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration (2013)
Book Chapter
McCarron, J., Olson, M., Chambers, S., & Girkin, J. (2013). Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration. In D. G. Lambert, & R. D. Rainbow (Eds.), Calcium signaling protocols (239-251). Springer Verlag. https://doi.org/10.1007/978-1-62703-086-1_15

Increases in bulk average cytoplasmic Ca2+ concentration ([Ca2+]c) are derived from the combined activities of many Ca2+ channels. Near (<100 nm) the mouth of each of these channels the local [Ca2+]c rises and falls more quickly and reaches much grea...

Laser-targeted ablation of the zebrafish embryonic ventricle: A novel model of cardiac injury and repair (2013)
Journal Article
Matrone, G., Taylor, J. M., Wilson, K. S., Baily, J., Love, G. D., Girkin, J. M., …Denvir, M. A. (2013). Laser-targeted ablation of the zebrafish embryonic ventricle: A novel model of cardiac injury and repair. International Journal of Cardiology, 168(4), 3913-3919. https://doi.org/10.1016/j.ijcard.2013.06.063

Background: While the adult zebrafish (Danio rerio) heart demonstrates a remarkable capacity for self-renewal following apical resection little is known about the response to injury in the embryonic heart. Methods: Injury to the beating zebrafish emb... Read More about Laser-targeted ablation of the zebrafish embryonic ventricle: A novel model of cardiac injury and repair.

From Structure to Function: Mitochondrial Morphology, Motion and Shaping in Vascular Smooth Muscle (2013)
Journal Article
McCarron, J. G., Wilson, C., Sandison, M. E., Olson, M. L., Girkin, J. M., Saunter, C., & Chalmers, S. (2013). From Structure to Function: Mitochondrial Morphology, Motion and Shaping in Vascular Smooth Muscle. Journal of Vascular Research, 50(5), 357-371. https://doi.org/10.1159/000353883

The diversity of mitochondrial arrangements, which arise from the organelle being static or moving, or fusing and dividing in a dynamically reshaping network, is only beginning to be appreciated. While significant progress has been made in understand... Read More about From Structure to Function: Mitochondrial Morphology, Motion and Shaping in Vascular Smooth Muscle.