Professor Sushma Grellscheid's Outputs (5)

A bending rigidity parameter for stress granule condensates (2023)
Journal Article
Law, J. O., Jones, C. M., Stevenson, T., Williamson, T. A., Turner, M. S., Kusumaatmaja, H., & Grellscheid, S. N. (2023). A bending rigidity parameter for stress granule condensates. Science Advances, 9(20), https://doi.org/10.1126/sciadv.adg0432

Interfacial tension plays an important role in governing the dynamics of droplet coalescence and determining how condensates interact with and deform lipid membranes and biological filaments. We demonstrate that an interfacial tension-only model is i... Read More about A bending rigidity parameter for stress granule condensates.

Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation (2020)
Journal Article
Vietri, M., Schultz, S. W., Bellanger, A., Jones, C. M., Petersen, L. I., Raiborg, C., Skarpen, E., Pedurupillay, C. R. J., Kjos, I., Kip, E., Timmer, R., Jain, A., Collas, P., Knorr, R. L., Grellscheid, S. N., Kusumaatmaja, H., Brech, A., Micci, F., Stenmark, H., & Campsteijn, C. (2020). Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation. Nature Cell Biology, 22(7), 856-867. https://doi.org/10.1038/s41556-020-0537-5

The ESCRT-III membrane fission machinery maintains the integrity of the nuclear envelope. Although primary nuclei resealing takes minutes, micronuclear envelope ruptures seem to be irreversible. Instead, micronuclear ruptures result in catastrophic m... Read More about Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.

The splicing landscape is globally reprogrammed during male meiosis (2013)
Journal Article
Schmid, R., Grellscheid, S., Ehrmann, I., Dalgliesh, C., Danilenko, M., Paronetto, M., …Elliott, D. (2013). The splicing landscape is globally reprogrammed during male meiosis. Nucleic Acids Research, 41(22), 10170-10184. https://doi.org/10.1093/nar/gkt811

Meiosis requires conserved transcriptional changes, but it is not known whether there is a corresponding set of RNA splicing switches. Here, we used RNAseq of mouse testis to identify changes associated with the progression from mitotic spermatogonia... Read More about The splicing landscape is globally reprogrammed during male meiosis.

Identification of evolutionarily conserved exons as regulated targets for the splicing activator tra2β in development (2011)
Journal Article
Grellscheid, S., Dalgliesh, C., Storbeck, M., Best, A., Liu, Y., Jakubik, M., Mende, Y., Ehrmann, I., Curk, T., Rossbach, K., Bourgeois, C. F., Stévenin, J., Grellscheid, D., Jackson, M. S., Wirth, B., & Elliott, D. J. (2011). Identification of evolutionarily conserved exons as regulated targets for the splicing activator tra2β in development. PLoS Genetics, 7(12), Article e1002390. https://doi.org/10.1371/journal.pgen.1002390

Alternative splicing amplifies the information content of the genome, creating multiple mRNA isoforms from single genes. The evolutionarily conserved splicing activator Tra2β (Sfrs10) is essential for mouse embryogenesis and implicated in spermatogen... Read More about Identification of evolutionarily conserved exons as regulated targets for the splicing activator tra2β in development.

Molecular design of a splicing switch responsive to the RNA binding protein Tra2β (2011)
Journal Article
Grellscheid, S., Dalgliesh, C., Rozanska, A., Grellscheid, D., Bourgeois, C., Stévenin, J., & Elliott, D. (2011). Molecular design of a splicing switch responsive to the RNA binding protein Tra2β. Nucleic Acids Research, 39(18), 8092-8104. https://doi.org/10.1093/nar/gkr495

Tra2β regulates a number of splicing switches including activation of the human testis-specific exon HIPK3-T in the Homeodomain Interacting Protein Kinase 3 gene. By testing HIPK3-T exons of different intrinsic strengths, we found Tra2β most efficien... Read More about Molecular design of a splicing switch responsive to the RNA binding protein Tra2β.