Professor Robert Pal's Outputs (91)

π‐Expanded Thioxanthones ‐ Engineering the Triplet Level of Thioxanthone Sensitizers for Lanthanide‐Based Luminescent Probes with Visible Excitation (2019)
Journal Article
Dansholm, C. N., Junker, A. K. R., Nielsen, L. G., Kofod, N., Pal, R., & Sørensen, T. J. (2019). π‐Expanded Thioxanthones ‐ Engineering the Triplet Level of Thioxanthone Sensitizers for Lanthanide‐Based Luminescent Probes with Visible Excitation. ChemPlusChem, 84(12), 1778-1788. https://doi.org/10.1002/cplu.201900309

Bright lanthanide based probes for optical bioimaging must rely on the antenna principle, where the lanthanide‐centred excited state is formed by a complex sensitization process. Efficient sensitization of lanthanide‐centred emission occurs via tripl... Read More about π‐Expanded Thioxanthones ‐ Engineering the Triplet Level of Thioxanthone Sensitizers for Lanthanide‐Based Luminescent Probes with Visible Excitation.

Near Infrared Light Activates Molecular Nanomachines to Drill Into and Kill Cells (2019)
Journal Article
Liu, D., García-López, V., Gunasekera, R. S., Greer Nilewski, L., Alemany, L. B., Aliyan, A., Jin, T., Wang, G., Tour, J. M., & Pal, R. (2019). Near Infrared Light Activates Molecular Nanomachines to Drill Into and Kill Cells. ACS Nano, 13(6), 6813-6823. https://doi.org/10.1021/acsnano.9b01556

Using two-photon excitation (2PE), molecular nanomachines (MNMs) are able to drill through cell membranes and kill the cells. This avoids the use of the more damaging ultraviolet (UV) light that has been used formerly to induce this nanomechanical ce... Read More about Near Infrared Light Activates Molecular Nanomachines to Drill Into and Kill Cells.

Holographic projection for multi-spot structured illumination microscopy (2019)
Journal Article
Ward, E., & Pal, R. (2019). Holographic projection for multi-spot structured illumination microscopy. Journal of Microscopy, 274(2), 114-120. https://doi.org/10.1111/jmi.12787

This paper describes the design and testing of a multi‐spot structured illumination microscopy system using computer‐generated holograms to create the required excitation patterns. Furthermore, it demonstrates the use of an adapted direct search algo... Read More about Holographic projection for multi-spot structured illumination microscopy.

In Vitro and in Cellulo Sensing of Transition Metals Using Time-Resolved Fluorescence Spectroscopy and Microscopy (2018)
Journal Article
Pal, R., Barker, A. C., Hummel, D., & Pålsson, L. (2019). In Vitro and in Cellulo Sensing of Transition Metals Using Time-Resolved Fluorescence Spectroscopy and Microscopy. Journal of Fluorescence, 29(1), 255-263. https://doi.org/10.1007/s10895-018-2335-z

In this work we demonstrate that time domain techniques can be used successfully to monitor realtively weak modulations of the fluorescence in sensing applications. The metal sensing complex Newport Green DCF™ can detect selected transition metals in... Read More about In Vitro and in Cellulo Sensing of Transition Metals Using Time-Resolved Fluorescence Spectroscopy and Microscopy.

Interaction of Nucleotides with a Trinuclear Terbium(III)–Dizinc(II) Complex: Efficient Sensitization of Terbium Luminescence by Guanosine Monophosphate and Application to Real-Time Monitoring of Phosphodiesterase Activity (2018)
Journal Article
Aulsebrook, M. L., Starck, M., Grace, M. R., Graham, B., Thordarson, P., Pal, R., & Tuck, K. L. (2019). Interaction of Nucleotides with a Trinuclear Terbium(III)–Dizinc(II) Complex: Efficient Sensitization of Terbium Luminescence by Guanosine Monophosphate and Application to Real-Time Monitoring of Phosphodiesterase Activity. Inorganic Chemistry, 58(1), 495-505. https://doi.org/10.1021/acs.inorgchem.8b02731

An in-depth study of the interaction of a trinuclear terbium(III)–dizinc(II) complex with an array of nucleotides differing in the type of nucleobase and number of phosphate groups, as well as cyclic versus acyclic variants, is presented. The study e... Read More about Interaction of Nucleotides with a Trinuclear Terbium(III)–Dizinc(II) Complex: Efficient Sensitization of Terbium Luminescence by Guanosine Monophosphate and Application to Real-Time Monitoring of Phosphodiesterase Activity.

Highly Linearized Twisted Iridium(III) Complexes (2018)
Journal Article
Davidson, R., Hsu, Y.-T., Griffiths, G. C., Li, C., Yufit, D., Pal, R., & Beeby, A. (2018). Highly Linearized Twisted Iridium(III) Complexes. Inorganic Chemistry, 57(22), 14450-14462. https://doi.org/10.1021/acs.inorgchem.8b02818

Sonogashira coupling with alkyne iridium(phenylpyridine)2(acetylacetone) synthons is used to produce highly linearized twisted iridium(III) complexes with lengths of up to 5 nm. These complexes were mechanically aligned in a polymer matrix, showing a... Read More about Highly Linearized Twisted Iridium(III) Complexes.

Selective signalling of glyphosate in water using europium luminescence (2018)
Journal Article
Jennings, L. B., Shuvaev, S., Fox, M. A., Pal, R., & Parker, D. (2018). Selective signalling of glyphosate in water using europium luminescence. Dalton Transactions, 47(45), 16145-16154. https://doi.org/10.1039/c8dt03823f

A series of four emissive europium complexes has been evaluated for the binding of glyphosate in various aqueous media, including river water and grain extracts. Binding selectivity toward inorganic phosphate and bicarbonate was enhanced by measuring... Read More about Selective signalling of glyphosate in water using europium luminescence.

Cationic Europium Complexes for Visualizing Fluctuations in Mitochondrial ATP Levels in Living Cells (2018)
Journal Article
Mailhot, R., Traviss-Pollard, T., Pal, R., & Butler, S. J. (2018). Cationic Europium Complexes for Visualizing Fluctuations in Mitochondrial ATP Levels in Living Cells. Chemistry - A European Journal, 24(42), 10745-10755. https://doi.org/10.1002/chem.201801008

The ability to study cellular metabolism and enzymatic processes involving adenosine triphosphate (ATP) is impeded by the lack of imaging probes capable of signalling the concentration and distribution of intracellular ATP rapidly, with high sensitiv... Read More about Cationic Europium Complexes for Visualizing Fluctuations in Mitochondrial ATP Levels in Living Cells.

On the antibacterial activity of azacarboxylate ligands: lowered metal ion affinities for bis-amide derivatives of EDTA do not mean reduced activity (2018)
Journal Article
Mulla, R. S., Beecroft, M. S., Pal, R., Aguilar, J., Pitarch-Jarque, J., García‐España, E., Lurie-Luke, E., Sharples, G., & Williams, J. (2018). On the antibacterial activity of azacarboxylate ligands: lowered metal ion affinities for bis-amide derivatives of EDTA do not mean reduced activity. Chemistry - A European Journal, 24(28), 7137-7148. https://doi.org/10.1002/chem.201800026

EDTA is widely used as an inhibitor of bacterial growth, affecting the uptake and control of metal ions by microorganisms. We describe the synthesis and characterisation of two symmetrical bis‐amide derivatives of EDTA, featuring glycyl or pyridyl su... Read More about On the antibacterial activity of azacarboxylate ligands: lowered metal ion affinities for bis-amide derivatives of EDTA do not mean reduced activity.

Enhancing multi-spot structured illumination microscopy with fluorescence difference (2018)
Journal Article
Ward, E. N., Torkelsen, F. H., & Pal, R. (2018). Enhancing multi-spot structured illumination microscopy with fluorescence difference. Royal Society Open Science, 5(3), Article 171336. https://doi.org/10.1098/rsos.171336

Structured illumination microscopy is a super-resolution technique used extensively in biological research. However, this technique is limited in the maximum possible resolution increase. Here we report the results of simulations of a novel enhanced... Read More about Enhancing multi-spot structured illumination microscopy with fluorescence difference.

Enantioselective cellular localisation of europium(iii) coordination complexes (2017)
Journal Article
Frawley, A. T., Linford, H., Starck, M., Pal, R., & Parker, D. (2018). Enantioselective cellular localisation of europium(iii) coordination complexes. Chemical Science, 9(4), 1042-1049. https://doi.org/10.1039/c7sc04422d

The selective mitochondrial localisation of the Λ enantiomer of three different emissive europium(III) complexes in NIH 3T3 and MCF7 cells contrasts with the behaviour of the Δ enantiomer, for which a predominant lysosomal localisation was observed b... Read More about Enantioselective cellular localisation of europium(iii) coordination complexes.

Chiral transcription in self-assembled tetrahedral Eu4L6 chiral cages displaying sizable circularly polarized luminescence (2017)
Journal Article
Yeung, C., Yim, K., Wong, H., Pal, R., Lo, W., Yan, S., …Law, G. (2017). Chiral transcription in self-assembled tetrahedral Eu4L6 chiral cages displaying sizable circularly polarized luminescence. Nature Communications, 8(1), Article 1128. https://doi.org/10.1038/s41467-017-01025-1

Predictable stereoselective formation of supramolecular assembly is generally believed to be an important but complicated process. Here, we show that point chirality of a ligand decisively influences its supramolecular assembly behavior. We designed... Read More about Chiral transcription in self-assembled tetrahedral Eu4L6 chiral cages displaying sizable circularly polarized luminescence.

Molecular machines open cell membranes (2017)
Journal Article
García-López, V., Chen, F., Nilewski, L. G., Duret, G., Aliyan, A., Kolomeisky, A. B., …Tour, J. M. (2017). Molecular machines open cell membranes. Nature, 548(7669), 567-572. https://doi.org/10.1038/nature23657

Beyond the more common chemical delivery strategies, several physical techniques are used to open the lipid bilayers of cellular membranes. These include using electric and magnetic fields, temperature, ultrasound or light to introduce compounds into... Read More about Molecular machines open cell membranes.

Circularly Polarised Luminescence from Helically Chiral “Confused” N,N,O,C-Boron-Chelated Dipyrromethenes (BODIPYs) (2017)
Journal Article
Clarke, R., Ho, K. L., Alsimaree, A. A., Woodford, O. J., Waddell, P. G., Bogaerts, J., …Hall, M. J. (2017). Circularly Polarised Luminescence from Helically Chiral “Confused” N,N,O,C-Boron-Chelated Dipyrromethenes (BODIPYs). ChemPhotoChem, 1(11), 513-517. https://doi.org/10.1002/cptc.201700106

Chiral organic fluorophores have significant promise in the development of efficient emitters of circularly polarized light. Herein we describe a helically chiral boron dipyrromethene (BODIPY) with a hitherto unreported N,N,O,C-boron-chelation motif,... Read More about Circularly Polarised Luminescence from Helically Chiral “Confused” N,N,O,C-Boron-Chelated Dipyrromethenes (BODIPYs).

Image scanning microscopy: an overview (2017)
Journal Article
Ward, E., & Pal, R. (2017). Image scanning microscopy: an overview. Journal of Microscopy, 266(2), 221-228. https://doi.org/10.1111/jmi.12534

For almost a century, the resolution of optical microscopy was thought to be limited by Abbé’s law describing the diffraction limit of light. At the turn of the millennium, aided by new technologies and fluorophores, the field of optical microscopy f... Read More about Image scanning microscopy: an overview.

A tight tunable range for Ni(II) sensing and buffering in cells (2017)
Journal Article
Foster, A., Pernil, R., Patterson, C., Scott, A., Pålsson, L., Pal, R., …Robinson, N. (2017). A tight tunable range for Ni(II) sensing and buffering in cells. Nature Chemical Biology, 13(4), 409-414. https://doi.org/10.1038/nchembio.2310

The metal affinities of metal-sensing transcriptional regulators co-vary with cellular metal concentrations over more than 12 orders of magnitude. To understand the cause of this relationship, we determined the structure of the Ni(II) sensor InrS and... Read More about A tight tunable range for Ni(II) sensing and buffering in cells.

Induced Europium Circularly Polarised Luminescence Monitors Reversible Drug Binding to Native α1-Acid Glycoprotein (2017)
Journal Article
Jennings, L., Waters, R., Pal, R., & Parker, D. (2017). Induced Europium Circularly Polarised Luminescence Monitors Reversible Drug Binding to Native α1-Acid Glycoprotein. ChemMedChem, 12(3), 271-277. https://doi.org/10.1002/cmdc.201600571

Alpha-1-acid glycoprotein (α1-AGP) is an important blood plasma glycoprotein. Following an acute-phase reaction such as stress, inflammation, burn, or infection, the bloodstream concentration of α1-AGP can increase up to 400 % of its normal concentra... Read More about Induced Europium Circularly Polarised Luminescence Monitors Reversible Drug Binding to Native α1-Acid Glycoprotein.

Very bright, enantiopure europium(III) complexes allow time-gated chiral contrast imaging (2016)
Journal Article
Frawley, A., Pal, D., & Parker, D. (2016). Very bright, enantiopure europium(III) complexes allow time-gated chiral contrast imaging. Chemical Communications, 52(91), 13349-13352. https://doi.org/10.1039/c6cc07313a

Chiral image contrast is demonstrated using enantiopure Eu(III) complexes that emit right or left-handed circularly polarised light of opposite sign, at selected wavelengths.

Novel aminoalkyl tris-cyclometalated iridium complexes as cellular stains (2016)
Journal Article
Sansee, A., Meksawangwong, S., Chainok, K., Franz, K., Gal, M., Palsson, L. -., …Kielar, F. (2016). Novel aminoalkyl tris-cyclometalated iridium complexes as cellular stains. Dalton Transactions, 45(43), 17420-17430. https://doi.org/10.1039/c6dt02776h

Herein we report the synthesis and investigation of the properties of two tris-cyclometalated luminescent iridium complexes. These complexes are the simple derivatives of fac-[Ir(ppy)3] bearing amino alkyl groups on one of the phenylpyridine rings. T... Read More about Novel aminoalkyl tris-cyclometalated iridium complexes as cellular stains.

New Class of Bright and Highly Stable Chiral Cyclen Europium Complexes for Circularly Polarized Luminescence Applications (2016)
Journal Article
Dai, L., Lo, W., Coates, I. D., Pal, R., & Law, G. (2016). New Class of Bright and Highly Stable Chiral Cyclen Europium Complexes for Circularly Polarized Luminescence Applications. Inorganic Chemistry, 55(17), 9065-9070. https://doi.org/10.1021/acs.inorgchem.6b01546

High glum values of +0.30 (ΔJ = 1, 591 nm, in DMSO) and −0.23 (ΔJ = 1, 589 nm, in H2O) were recorded in our series of newly designed macrocyclic europium(III) complexes. A sterically locking approach involving a bidentate chromophore is adopted to co... Read More about New Class of Bright and Highly Stable Chiral Cyclen Europium Complexes for Circularly Polarized Luminescence Applications.