Dr Gary Sharples' Outputs (4)

A model for dsDNA translocation revealed by a structural motif common to RecG and Mfd proteins. (2003)
Journal Article
Mahdi, A., Briggs, G., Sharples, G., Wen, Q., & Lloyd, R. (2003). A model for dsDNA translocation revealed by a structural motif common to RecG and Mfd proteins. The EMBO Journal, 22, 724-734

RecG protein differs from other helicases analysed to atomic resolution in that it mediates strand separation via translocation on double-stranded (ds) rather than single-stranded (ss) DNA. We describe a highly conserved helical hairpin motif in RecG... Read More about A model for dsDNA translocation revealed by a structural motif common to RecG and Mfd proteins..

The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold. (2003)
Journal Article
Rafferty, J., Bolt, E., Muranova, T., Sedelnikova, S., Leonard, P., Pasquo, A., …Lloyd, R. (2003). The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold. Structure, 11, 1557-1567

Holliday junction resolution performed by a variety of structure-specific endonucleases is a key step in DNA recombination and repair. It is believed that all resolvases carry out their reaction chemistries in a similar fashion, utilizing a divalent... Read More about The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold..

The RdgC protein of Escherichia coli binds DNA and counters a toxic effect of RecFOR in strains lacking the replication restart protein PriA. (2003)
Journal Article
Moore, T., McGlynn, P., Ngo, H., Sharples, G., & Lloyd, R. (2003). The RdgC protein of Escherichia coli binds DNA and counters a toxic effect of RecFOR in strains lacking the replication restart protein PriA. The EMBO Journal, 22, 735-745

PriA protein provides a means to load the DnaB replicative helicase at DNA replication fork and D loop structures, and is therefore a key factor in the rescue of stalled or broken forks and subsequent replication restart. We show that the nucleoid-as... Read More about The RdgC protein of Escherichia coli binds DNA and counters a toxic effect of RecFOR in strains lacking the replication restart protein PriA..

Helicobacter pylori mutants defective in RuvC Holliday junction resolvase display reduced macrophage survival and spontaneous clearance from the murine gastric mucosa. (2003)
Journal Article
Loughlin, M., Barnard, F., Jenkins, D., Sharples, G., & Jenks, P. (2003). Helicobacter pylori mutants defective in RuvC Holliday junction resolvase display reduced macrophage survival and spontaneous clearance from the murine gastric mucosa. Infection and Immunity, 71, 2022-2031

Homologous recombination contributes to the extraordinary genetic diversity of Helicobacter pylori and may be critical for surface antigen expression and adaptation to environmental challenges within the stomach. We generated isogenic, nonpolar H. py... Read More about Helicobacter pylori mutants defective in RuvC Holliday junction resolvase display reduced macrophage survival and spontaneous clearance from the murine gastric mucosa..