Dr Gary Sharples' Outputs (3)

Structural similarities between Escherichia coli RuvA protein and other DNA-binding proteins and a mutational analysis of its binding to the Holliday junction. (1998)
Journal Article
Rafferty, J., Ingleston, S., Hargreaves, D., Artymiuk, P., Sharples, G., Lloyd, R., & Rice, D. (1998). Structural similarities between Escherichia coli RuvA protein and other DNA-binding proteins and a mutational analysis of its binding to the Holliday junction

Comparison of the structure of Escherichia coli RuvA with other proteins in the Protein Data Bank gives insights into the probable modes of association of RuvA with the Holliday junction during homologous recombination. All three domains of the RuvA... Read More about Structural similarities between Escherichia coli RuvA protein and other DNA-binding proteins and a mutational analysis of its binding to the Holliday junction..

Lambda Rap protein is a structure-specific endonuclease involved in phage recombination. (1998)
Journal Article
Sharples, G., Corbett, L., & Graham, I. (1998). Lambda Rap protein is a structure-specific endonuclease involved in phage recombination

Bacteriophage lambda encodes a number of genes involved in the recombinational repair of DNA double-strand breaks. The product of one of these genes, rap, has been purified. Truncated Rap proteins that copurify with the full-length form are derived,... Read More about Lambda Rap protein is a structure-specific endonuclease involved in phage recombination..

Sequence-specificity of Holliday junction resolution: identification of RuvC mutants defective in metal binding and target site recognition. (1998)
Journal Article
Hagan, N., Vincent, S., Ingleston, S., Sharples, G., Bennett, R., West, S., & Lloyd, R. (1998). Sequence-specificity of Holliday junction resolution: identification of RuvC mutants defective in metal binding and target site recognition. Journal of Molecular Biology, 281, 17-29

The RuvC protein of Escherichia coli resolves Holliday intermediates in recombination and DNA repair by a dual strand incision mechanism targeted to specific DNA sequences located symmetrically at the crossover. Two classes of amino acid substitution... Read More about Sequence-specificity of Holliday junction resolution: identification of RuvC mutants defective in metal binding and target site recognition..