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Dr Gary Sharples' Outputs (3)

Cloning, overexpression, purification, and characterization of the Escherichia coli RuvC Holliday junction resolvase. (1994)
Journal Article
Dunderdale, H., Sharples, G., Lloyd, R., & West, S. (1994). Cloning, overexpression, purification, and characterization of the Escherichia coli RuvC Holliday junction resolvase. Journal of Biological Chemistry, 269(7), 5187-5194

The ruvC gene has been cloned into the plasmid pT7-7 under the control of the T7 phi 10 promoter. Following induction with isopropyl-1-thio-beta-D-galactopyranoside, the 19-kDa RuvC protein was overexpressed to 20-30% of total cell protein. RuvC has... Read More about Cloning, overexpression, purification, and characterization of the Escherichia coli RuvC Holliday junction resolvase..

A mutation in helicase motif III of E. coli RecG protein abolishes branch migration of Holliday junctions. (1994)
Journal Article
Sharples, G., Whitby, M., Ryder, L., & Lloyd, R. (1994). A mutation in helicase motif III of E. coli RecG protein abolishes branch migration of Holliday junctions. Nucleic Acids Research, 22(3), 308-313

The RecG protein of Escherichia coli catalyses branch migration of Holliday junctions made by RecA and dissociates synthetic X junctions into duplex products in reactions that require hydrolysis of ATP. To investigate the mode of action of this enzym... Read More about A mutation in helicase motif III of E. coli RecG protein abolishes branch migration of Holliday junctions..

Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions. (1994)
Journal Article
Sharples, G., Chan, S., Mahdi, A., Whitby, M., & Lloyd, R. (1994). Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions. The EMBO Journal, 13(24), 6133-6142

The formation and subsequent resolution of Holliday junctions are critical stages in recombination. We describe a new Escherichia coli endonuclease that resolves Holliday intermediates by junction cleavage. The 14 kDa Rus protein binds DNA containing... Read More about Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions..