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All Outputs (7)

Photoactivated cell-killing involving a low molecular weight, donor-acceptor diphenylacetylene (2019)
Journal Article
Chisholm, D., Lamb, R., Pallett, T., Affleck, V., Holden, C., Marrison, J., O'Toole, P., Ashton, P., Newling, K., Steffen, A., Nelson, A., Mahler, C., Valentine, R., Blacker, T., Bain, A. J., Girkin, J. M., Marder, T. B., Whiting, A., & Ambler, C. A. (2019). Photoactivated cell-killing involving a low molecular weight, donor-acceptor diphenylacetylene. Chemical Science, 10(17), 4673-4683. https://doi.org/10.1039/c9sc00199a

Photoactivation of photosensitisers can be utilised to elicit the production of ROS, for potential therapeutic applications, including the destruction of diseased tissues and tumours. A novel class of photosensitiser, exemplified by DC324, has been d... Read More about Photoactivated cell-killing involving a low molecular weight, donor-acceptor diphenylacetylene.

Fluorescent retinoic acid analogues as probes for biochemical and intracellular characterization of retinoid signalling pathways (2019)
Journal Article
Chisholm, D., Tomlinson, C., Zhou, G.-L., Holden, C., Affleck, V., Lamb, R., Newling, K., Ashton, P., Valentine, R., Redfern, C., Erostyak, J., Makkai, G., Ambler, C., Whiting, A., & Pohl, E. (2019). Fluorescent retinoic acid analogues as probes for biochemical and intracellular characterization of retinoid signalling pathways. ACS Chemical Biology, 14(3), 369-377. https://doi.org/10.1021/acschembio.8b00916

Retinoids, such as all-trans-retinoic acid (ATRA), are endogenous signalling molecules derived from Vitamin A that influ-ence a variety of cellular processes through mediation of transcription events in the cell nucleus. Due to these wide-ranging and... Read More about Fluorescent retinoic acid analogues as probes for biochemical and intracellular characterization of retinoid signalling pathways.

Manufacture of Third-Generation Lentivirus for Preclinical Use, with Process Development Considerations for Translation to Good Manufacturing Practice (2017)
Journal Article
Gándara, C., Affleck, V., & Stoll, E. A. (2018). Manufacture of Third-Generation Lentivirus for Preclinical Use, with Process Development Considerations for Translation to Good Manufacturing Practice. Human gene therapy, Part B. Methods, 29(1), https://doi.org/10.1089/hgtb.2017.098

Lentiviral vectors are used in laboratories around the world for in vivo and ex vivo delivery of gene therapies, and increasingly clinical investigation as well as preclinical applications. The third-generation lentiviral vector system has many advan... Read More about Manufacture of Third-Generation Lentivirus for Preclinical Use, with Process Development Considerations for Translation to Good Manufacturing Practice.

Fatty acid oxidation is required for the respiration and proliferation of malignant glioma cells (2016)
Journal Article
Lin, H., Patel, S., Affleck, V. S., Wilson, I., Turnbull, D. M., Joshi, A. R., Maxwell, R., & Stoll, E. A. (2017). Fatty acid oxidation is required for the respiration and proliferation of malignant glioma cells. Neuro-Oncology, 19(1), 43-54. https://doi.org/10.1093/neuonc/now128

Background.
Glioma is the most common form of primary malignant brain tumor in adults, with approximately 4 cases per 100 000 people each year. Gliomas, like many tumors, are thought to primarily metabolize glucose for energy production; however, th... Read More about Fatty acid oxidation is required for the respiration and proliferation of malignant glioma cells.

The projection and synaptic organisation of NTS afferent connections with presympathetic neurones, GABA and nNOS neurons in the paraventricular nucleus of the hypothalamus (2012)
Journal Article
Affleck, V., Coote, J., & Pyner, S. (2012). The projection and synaptic organisation of NTS afferent connections with presympathetic neurones, GABA and nNOS neurons in the paraventricular nucleus of the hypothalamus. Neuroscience, 219, 48-61. https://doi.org/10.1016/j.neuroscience.2012.05.070

Elevated sympathetic nerve activity, strongly associated with cardiovascular disease, is partly generated from the presympathetic neurons of the paraventricular nucleus of the hypothalamus (PVN). The PVN-presympathetic neurons regulating cardiac and... Read More about The projection and synaptic organisation of NTS afferent connections with presympathetic neurones, GABA and nNOS neurons in the paraventricular nucleus of the hypothalamus.