Skip to main content

Research Repository

Advanced Search

All Outputs (143)

MicroRNAs in the skin: role in development, homoeostasis and regeneration (2017)
Journal Article
Horsburgh, S., Fullard, N., Roger, M., Degnan, A., Todryk, S., Przyborski, S., & O’Reilly, S. (2017). MicroRNAs in the skin: role in development, homoeostasis and regeneration. Clinical Science, 131(15), 1923-1940. https://doi.org/10.1042/cs20170039

The skin is the largest organ of the integumentary system and possesses a vast number of functions. Due to the distinct layers of the skin and the variety of cells which populate each, a tightly regulated network of molecular signals control developm... Read More about MicroRNAs in the skin: role in development, homoeostasis and regeneration.

Systems modelling ageing: from single senescent cells to simple multi-cellular models (2017)
Journal Article
Martinez Guimera, A., Welsh, C., Dalle Pezze, P., Fullard, N., Nelson, G., Roger, M., …Shanley, D. (2017). Systems modelling ageing: from single senescent cells to simple multi-cellular models. Essays in Biochemistry, 61(3), 369-377. https://doi.org/10.1042/ebc20160087

Systems modelling has been successfully used to investigate several key molecular mechanisms of ageing. Modelling frameworks to allow integration of models and methods to enhance confidence in models are now well established. In this article, we disc... Read More about Systems modelling ageing: from single senescent cells to simple multi-cellular models.

Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis (2017)
Journal Article
Yang, C., Xu, Y., Yu, M., Lee, D., Alharti, S., Hellen, N., …Armstrong, L. (2017). Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis. Human Molecular Genetics, 26(16), 3031-3045. https://doi.org/10.1093/hmg/ddx140

Hypoplastic left heart syndrome (HLHS) is among the most severe forms of congenital heart disease. Although the consensus view is that reduced flow through the left heart during development is a key factor in the development of the condition, the mol... Read More about Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis.

Neurogenesis in Response to Synthetic Retinoids at Different Temporal Scales (2017)
Journal Article
Haffez, H., Khatib, T., McCaffery, P., Przyborski, S., Redfern, C., & Whiting, A. (2018). Neurogenesis in Response to Synthetic Retinoids at Different Temporal Scales. Molecular Neurobiology, 55(3), 1942-1950. https://doi.org/10.1007/s12035-017-0440-7

All-trans retinoic acid (ATRA) plays key roles in neurogenesis mediated by retinoic acid receptors (RARs). RARs are important targets for the therapeutic regulation of neurogenesis but effective drug development depends on modelling-based strategies... Read More about Neurogenesis in Response to Synthetic Retinoids at Different Temporal Scales.

Advanced cell culture technology for generation of in vivo-like tissue models (2017)
Journal Article
Przyborski, S. (2017). Advanced cell culture technology for generation of in vivo-like tissue models. International journal of health animal science & food safety, 4(1), https://doi.org/10.13130/2283-3927/8524

Human tissues are mostly composed of different cell types, that are often highly organised in relation to each other. Often cells are arranged in distinct layers that enable signalling and cell-to-cell interactions. Here we describe the application o... Read More about Advanced cell culture technology for generation of in vivo-like tissue models.

A robust and reproducible human pluripotent stem cell derived model of neurite outgrowth in a three-dimensional culture system and its application to study neurite inhibition (2016)
Journal Article
Clarke, K., Tams, D., Henderson, A., Roger, M., Whiting, A., & Przyborski, S. (2016). A robust and reproducible human pluripotent stem cell derived model of neurite outgrowth in a three-dimensional culture system and its application to study neurite inhibition. Neurochemistry International, 106, 74-84. https://doi.org/10.1016/j.neuint.2016.12.009

The inability of neurites to grow and restore neural connections is common to many neurological disorders, including trauma to the central nervous system and neurodegenerative diseases. Therefore, there is need for a robust and reproducible model of... Read More about A robust and reproducible human pluripotent stem cell derived model of neurite outgrowth in a three-dimensional culture system and its application to study neurite inhibition.

Histone demethylation and toll‐like receptor 8–dependent cross‐talk in monocytes promotes transdifferentiation of fibroblasts in systemic sclerosis via Fra‐2 (2016)
Journal Article
Ciechomska, M., O'Reilly, S., Przyborski, S., Oakley, F., Bogunia-Kubik, K., & van Laar, J. (2016). Histone demethylation and toll‐like receptor 8–dependent cross‐talk in monocytes promotes transdifferentiation of fibroblasts in systemic sclerosis via Fra‐2. Arthritis and Rheumatology, 68(6), 1493-1504. https://doi.org/10.1002/art.39602

Objectives: To investigate whether epigenetic changes can modulate monocytes to produce tissue-inhibitor of metalloproteinase-1 (TIMP-1) via Fra2 (AP-1 family member), a novel downstream mediator promoting fibrogenesis. Methods: AP-1 transcription fa... Read More about Histone demethylation and toll‐like receptor 8–dependent cross‐talk in monocytes promotes transdifferentiation of fibroblasts in systemic sclerosis via Fra‐2.

IL-13 mediates collagen deposition via STAT6 and microRNA-135b: a role for epigenetics (2016)
Journal Article
O’Reilly, S., Ciechomska, M., Fullard, N., Przyborski, S., & van Laar, J. (2016). IL-13 mediates collagen deposition via STAT6 and microRNA-135b: a role for epigenetics. Scientific Reports, 6, Article 25066. https://doi.org/10.1038/srep25066

Systemic sclerosis is an autoimmune connective tissue disease in which T cells play a prominent role. We and others have previously demonstrated a role for T cell-derived IL-13 in mediating the induction of collagen in dermal fibroblasts and that blo... Read More about IL-13 mediates collagen deposition via STAT6 and microRNA-135b: a role for epigenetics.

Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro (2015)
Journal Article
Knight, E., & Przyborski, S. (2015). Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro. Journal of Anatomy, 227(6), 746-756. https://doi.org/10.1111/joa.12257

Research in mammalian cell biology often relies on developing in vitro models to enable the growth of cells in the laboratory to investigate a specific biological mechanism or process under different test conditions. The quality of such models and ho... Read More about Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro.

Using Zinc Finger Nuclease Technology to Generate CRX-Reporter Human Embryonic Stem Cells as a Tool to Identify and Study the Emergence of Photoreceptors Precursors During Pluripotent Stem Cell Differentiation (2015)
Journal Article
Collin, J., Mellough, C., Dorgau, B., Przyborski, S., Moreno-Gimeno, I., & Lako, M. (2016). Using Zinc Finger Nuclease Technology to Generate CRX-Reporter Human Embryonic Stem Cells as a Tool to Identify and Study the Emergence of Photoreceptors Precursors During Pluripotent Stem Cell Differentiation. Stem Cells, 34(2), 311-321. https://doi.org/10.1002/stem.2240

The purpose of this study was to generate human embryonic stem cell (hESC) lines harboring the green fluorescent protein (GFP) reporter at the endogenous loci of the Cone-Rod Homeobox (CRX) gene, a key transcription factor in retinal development. Zin... Read More about Using Zinc Finger Nuclease Technology to Generate CRX-Reporter Human Embryonic Stem Cells as a Tool to Identify and Study the Emergence of Photoreceptors Precursors During Pluripotent Stem Cell Differentiation.

Neuronal-glial populations form functional networks in a biocompatible 3D scaffold (2015)
Journal Article
Smith, I., Haag, M., Ugbode, C., Tams, D., Rattray, M., Przyborski, S., …Whalley, B. (2015). Neuronal-glial populations form functional networks in a biocompatible 3D scaffold. Neuroscience Letters, 609, 198-202. https://doi.org/10.1016/j.neulet.2015.10.044

Monolayers of neurons and glia have been employed for decades as tools for the study of cellular physiology and as the basis for a variety of standard toxicological assays. A variety of three dimensional (3D) culture techniques have been developed wi... Read More about Neuronal-glial populations form functional networks in a biocompatible 3D scaffold.

A novel fully-humanised 3D skin equivalent to model early melanoma invasion (2015)
Journal Article
Hill, D., Robinson, N., Caley, M., Chen, M., O'Toole, E., Armstrong, J., …Lovat, P. (2015). A novel fully-humanised 3D skin equivalent to model early melanoma invasion. Molecular Cancer Therapeutics, 14(11), 2665-2673. https://doi.org/10.1158/1535-7163.mct-15-0394

Metastatic melanoma remains incurable, emphasizing the acute need for improved research models to investigate the underlying biologic mechanisms mediating tumor invasion and metastasis, and to develop more effective targeted therapies to improve clin... Read More about A novel fully-humanised 3D skin equivalent to model early melanoma invasion.

Fully biodegradable and biocompatible emulsion template polymer scaffolds by thiol-acrylate polymerisation of polycaprolactone macropolymers (2015)
Journal Article
Johnson, D., Langford, C., Didsbury, M., Lipp, B., Przyborski, S., & Cameron, N. (2015). Fully biodegradable and biocompatible emulsion template polymer scaffolds by thiol-acrylate polymerisation of polycaprolactone macropolymers. Polymer Chemistry, 6(41), 7256-7263. https://doi.org/10.1039/c5py00721f

The emulsion templating process offers a route to highly porous polymers with well-defined morphologies. This study describes the preparation of such porous polymers (polyHIPEs) via the photopolymerization of a multi-functional thiol and polycaprolac... Read More about Fully biodegradable and biocompatible emulsion template polymer scaffolds by thiol-acrylate polymerisation of polycaprolactone macropolymers.

An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS-derived cardiac myocytes (2014)
Journal Article
Jiang, Y., Habibollah, S., Tilgner, K., Collin, J., Barta, T., Al-Aama, J., …Armstrong, L. (2014). An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS-derived cardiac myocytes. Stem Cells Translational Medicine, 3(4), 416-423. https://doi.org/10.5966/sctm.2013-0105

Hypoplastic left heart syndrome (HLHS) is a serious congenital cardiovascular malformation resulting in hypoplasia or atresia of the left ventricle, ascending aorta, and aortic and mitral valves. Diminished flow through the left side of the heart is... Read More about An induced pluripotent stem cell model of hypoplastic left heart syndrome (HLHS) reveals multiple expression and functional differences in HLHS-derived cardiac myocytes.

Application of Synthetic Photostable Retinoids Induces Novel Limb and Facial Phenotypes During Chick Embryogenesis In Vivo (2014)
Journal Article
Lopez-Real, R., Budge, J., Marder, T., Whiting, A., Hunt, P., & Przyborski, S. (2014). Application of Synthetic Photostable Retinoids Induces Novel Limb and Facial Phenotypes During Chick Embryogenesis In Vivo. Journal of Anatomy, 224(4), 392-411. https://doi.org/10.1111/joa.12147

We have recently developed a range of synthetic retinoid analogues which include the compounds EC23 and EC19. They are stable on exposure to light and are predicted to be resistant to the normal metabolic processes involved in the inactivation of ret... Read More about Application of Synthetic Photostable Retinoids Induces Novel Limb and Facial Phenotypes During Chick Embryogenesis In Vivo.

Brief report: a human induced pluripotent stem cell model of cernunnos deficiency reveals an important role for XLF in the survival of the primitive hematopoietic progenitors. (2013)
Journal Article
Tilgner, K., Neganova, I., Singhapol, C., Saretzki, G., Al-Aama, J., Evans, J., …Lako, M. (2013). Brief report: a human induced pluripotent stem cell model of cernunnos deficiency reveals an important role for XLF in the survival of the primitive hematopoietic progenitors. Stem Cells, 31(9), 2015-2023. https://doi.org/10.1002/stem.1456

A human iPSC model of Ligase IV deficiency reveals an important role for NHEJ-mediated-DSB repair in the survival and genomic stability of induced pluripotent stem cells and emerging haematopoietic progenitors. (2013)
Journal Article
Tilgner, K., Neganova, I., Moreno-Gimeno, I., Al-Aama, J., Burks, D., Yung, S., …Lako, M. (2013). A human iPSC model of Ligase IV deficiency reveals an important role for NHEJ-mediated-DSB repair in the survival and genomic stability of induced pluripotent stem cells and emerging haematopoietic progenitors. Cell Death & Differentiation, 20(8), 1089-1100. https://doi.org/10.1038/cdd.2013.44

Brief report: human pluripotent stem cell models of fanconi anemia deficiency reveal an important role for fanconi anemia proteins in cellular reprogramming and survival of hematopoietic progenitors (2013)
Journal Article
Yung, S., Tilgner, K., Ledran, M., Habibollah, S., Neganova, I., Singhapol, C., …Lako, M. (2013). Brief report: human pluripotent stem cell models of fanconi anemia deficiency reveal an important role for fanconi anemia proteins in cellular reprogramming and survival of hematopoietic progenitors. Stem Cells, 31(5), 1022-1029. https://doi.org/10.1002/stem.1308

Design and biological evaluation of synthetic retinoids: Probing length vs. stability vs. activity (2013)
Journal Article
Clemens, G., Flower, K., Gardner, P., Henderson, A., Knowles, J., Marder, T., …Przyborski, S. (2013). Design and biological evaluation of synthetic retinoids: Probing length vs. stability vs. activity. Molecular bioSystems, 9(12), 3124-3134. https://doi.org/10.1039/c3mb70273a

All trans-retinoic acid (ATRA) is widely used to direct the differentiation of cultured stem cells. When exposed to the pluripotent human embryonal carcinoma (EC) stem cell line, TERA2.cl.SP12, ATRA induces ectoderm differentiation and the formation... Read More about Design and biological evaluation of synthetic retinoids: Probing length vs. stability vs. activity.