All Outputs (126)

Suppressed basal mitophagy drives cellular aging phenotypes that can be reversed by a p62-targeting small molecule. (2024)
Journal Article
Kelly, G., Kataura, T., Panek, J., Ma, G., Salmonowicz, H., Davis, A., …Korolchuk, V. I. (2024). Suppressed basal mitophagy drives cellular aging phenotypes that can be reversed by a p62-targeting small molecule. Developmental Cell, Article S1534-5807(24)00295-8. https://doi.org/10.1016/j.devcel.2024.04.020

Selective degradation of damaged mitochondria by autophagy (mitophagy) is proposed to play an important role in cellular homeostasis. However, the molecular mechanisms and the requirement of mitochondrial quality control by mitophagy for cellular phy... Read More about Suppressed basal mitophagy drives cellular aging phenotypes that can be reversed by a p62-targeting small molecule..

Impact of the Physical Cellular Microenvironment on the Structure and Function of a Model Hepatocyte Cell Line for Drug Toxicity Applications (2023)
Journal Article
Allcock, B., Wei, W., Goncalves, K., Hoyle, H., Robert, A., Quelch-Cliffe, R., …Przyborski, S. (2023). Impact of the Physical Cellular Microenvironment on the Structure and Function of a Model Hepatocyte Cell Line for Drug Toxicity Applications. Cells, 12(19), Article 2408. https://doi.org/10.3390/cells12192408

It is widely recognised that cells respond to their microenvironment, which has implications for cell culture practices. Growth cues provided by 2D cell culture substrates are far removed from native 3D tissue structure in vivo. Geometry is one of ma... Read More about Impact of the Physical Cellular Microenvironment on the Structure and Function of a Model Hepatocyte Cell Line for Drug Toxicity Applications.

Generation of a novel three-dimensional scaffold-based model of the bovine endometrium (2023)
Journal Article
Díez, M., Przyborski, S., del Cerro, A., Alonso-Guervós, M., Iglesias-Cabo, T., Carrocera, S., …Muñoz, M. (2023). Generation of a novel three-dimensional scaffold-based model of the bovine endometrium. Veterinary Research Communications, 47(3), 1721-1733. https://doi.org/10.1007/s11259-023-10130-0

Bovine in vitro endometrial models that resemble tissue function in vivo are needed to study infertility, long-term uterine alterations induced by pathogens and impact of endocrine disruptor chemicals on reproductive function and other reproductive s... Read More about Generation of a novel three-dimensional scaffold-based model of the bovine endometrium.

Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III (2023)
Journal Article
Costello, L., Goncalves, K., De Los Santos Gomez, P., Simpson, A., Maltman, V., Ritchie, P., …Przyborski, S. (2023). Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III. Experimental Dermatology, 32(5), 620-631. https://doi.org/10.1111/exd.14754

Skin ageing is an intricate physiological process affected by intrinsic and extrinsic factors. There is a demand to understand how the skin changes with age and photoexposure in individuals with Fitzpatrick skin types I-III due to accelerated photoag... Read More about Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III.

Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23 (2023)
Journal Article
Määttä, A., Nixon, R., Robinson, N., Ambler, C. A., Goncalves, K., Maltman, V., & Przyborski, S. (2023). Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23. Journal of Cosmetic Dermatology, 22(5), https://doi.org/10.1111/jocd.15629

Background Retinoid signaling is an important regulator of the epidermis and skin appendages. Therefore, synthetic retinoids have been developed for therapeutic use for skin disorders such as psoriasis and acne. Aims In previous studies, we showed ho... Read More about Regulation of epidermal proliferation and hair follicle cycling by synthetic photostable retinoid EC23.

Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition (2022)
Journal Article
Goncalves, K., Phillips, S., Shah, D., Athey, D., & Przyborski, S. (2023). Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition. Biomaterials Advances, 144, Article 213204. https://doi.org/10.1016/j.bioadv.2022.213204

The microenvironment that cells experience during in vitro culture can often be far removed from the native environment they are exposed to in vivo. To recreate the physiological environment that developing neurites experience in vivo, we combine a w... Read More about Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition.

Investigation into the effect of skin tone modulators and exogenous stress on skin pigmentation utilizing a novel bioengineered skin equivalent (2022)
Journal Article
Goncalves, K., De Los Santos Gomez, P., Costello, L., Smith, L., Mead, H., Simpson, A., & Przyborski, S. (2023). Investigation into the effect of skin tone modulators and exogenous stress on skin pigmentation utilizing a novel bioengineered skin equivalent. Bioengineering and Translational Medicine, 8(2), Article e10415. https://doi.org/10.1002/btm2.10415

Human skin equivalents (HSEs) are a popular technology due to limitations in animal testing, particularly as they recapitulate aspects of structure and function of human skin. Many HSEs contain two basic cell types to model dermal and epidermal compa... Read More about Investigation into the effect of skin tone modulators and exogenous stress on skin pigmentation utilizing a novel bioengineered skin equivalent.

Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro (2022)
Journal Article
Hoyle, H., Stenger, C., & Przyborski, S. (2022). Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro. Biomaterials and biosystems, 8, Article 100063. https://doi.org/10.1016/j.bbiosy.2022.100063

One of the major aims of bio-engineering tissue equivalents in vitro is to create physiologically relevant culture conditions to accurately recreate the cellular microenvironment. This often includes incorporation of factors such as the extracellular... Read More about Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro.

Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging (2022)
Journal Article
Jarrold, B. B., Tan, C. Y. R., Ho, C. Y., Soon, A. L., Lam, T. T., Yang, X., …Oblong, J. E. (2022). Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging. Experimental Dermatology, 31(11), 1748-1760. https://doi.org/10.1111/exd.14654

Inflammaging is a theory of ageing which purports that low-level chronic inflammation leads to cellular dysfunction and premature ageing of surrounding tissue. Skin is susceptible to inflammaging because it is the first line of defence from the envir... Read More about Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging.

Recreating Tissue Structures Representative of Teratomas In Vitro Using a Combination of 3D Cell Culture Technology and Human Embryonic Stem Cells (2022)
Journal Article
Hidalgo Aguilar, A., Smith, L., Owens, D., Quelch, R., & Przyborski, S. (2022). Recreating Tissue Structures Representative of Teratomas In Vitro Using a Combination of 3D Cell Culture Technology and Human Embryonic Stem Cells. Bioengineering, 9(5), Article 185. https://doi.org/10.3390/bioengineering9050185

In vitro studies using human embryonic stem cells (hESCs) are a valuable method to study aspects of embryogenesis, avoiding ethical issues when using embryonic materials and species dissimilarities. The xenograft teratoma assay is often traditionally... Read More about Recreating Tissue Structures Representative of Teratomas In Vitro Using a Combination of 3D Cell Culture Technology and Human Embryonic Stem Cells.

Tissue engineering strategies to bioengineer the ageing skin phenotype in vitro (2022)
Journal Article
Costello, L., Dicolandrea, T., Tasseff, R., Isfort, R., Bascom, C., von Zglinicki, T., & Przyborski, S. (2022). Tissue engineering strategies to bioengineer the ageing skin phenotype in vitro. Aging Cell, 21(2), Article e13550. https://doi.org/10.1111/acel.13550

Human skin ageing is a complex and heterogeneous process, which is influenced by genetically determined intrinsic factors and accelerated by cumulative exposure to extrinsic stressors. In the current world ageing demographic, there is a requirement f... Read More about Tissue engineering strategies to bioengineer the ageing skin phenotype in vitro.

Development of a mammalian neurosensory full‐thickness skin equivalent and its application to screen sensitizing stimuli (2022)
Journal Article
Freer, M., Darling, N., Goncalves, K., Mills, K. J., & Przyborski, S. (2023). Development of a mammalian neurosensory full‐thickness skin equivalent and its application to screen sensitizing stimuli. Bioengineering and Translational Medicine, 8(3), Article e10484. https://doi.org/10.1002/btm2.10484

Human skin equivalents (HSEs) are an increasingly popular research tool due to limitations associated with animal testing for dermatological research. They recapitulate many aspects of skin structure and function, however, many only contain two basic... Read More about Development of a mammalian neurosensory full‐thickness skin equivalent and its application to screen sensitizing stimuli.

How good is the evidence that cellular senescence causes skin ageing? (2021)
Journal Article
Low, E., Alimohammadiha, G., Smith, L. A., Costello, L. F., Przyborski, S. A., von Zglinicki, T., & Miwa, S. (2021). How good is the evidence that cellular senescence causes skin ageing?. Ageing Research Reviews, 71, https://doi.org/10.1016/j.arr.2021.101456

Skin is the largest organ of the body with important protective functions, which become compromised with time due to both intrinsic and extrinsic ageing processes. Cellular senescence is the primary ageing process at cell level, associated with loss... Read More about How good is the evidence that cellular senescence causes skin ageing?.

The Cell-Permeable Derivative of the Immunoregulatory Metabolite Itaconate, 4-Octyl Itaconate, Is Anti-Fibrotic in Systemic Sclerosis (2021)
Journal Article
Henderson, J., Dayalan Naidu, S., Dinkova-Kostova, A. T., Przyborski, S., Stratton, R., & O′Reilly, S. (2021). The Cell-Permeable Derivative of the Immunoregulatory Metabolite Itaconate, 4-Octyl Itaconate, Is Anti-Fibrotic in Systemic Sclerosis. Cells, 10(8), https://doi.org/10.3390/cells10082053

Systemic sclerosis (SSc) is an autoimmune connective tissue disease that leads to skin fibrosis. Altered metabolism has recently been described in autoimmune diseases and SSc. Itaconate is a product of the Krebs cycle intermediate cis-aconitate and i... Read More about The Cell-Permeable Derivative of the Immunoregulatory Metabolite Itaconate, 4-Octyl Itaconate, Is Anti-Fibrotic in Systemic Sclerosis.

Bone Morphogenetic Protein Antagonist Gremlin-1 Increases Myofibroblast Transition in Dermal Fibroblasts: Implications for Systemic Sclerosis (2021)
Journal Article
Duffy, L., Henderson, J., Brown, M., Pryzborski, S., Fullard, N., Summa, L., …O’Reilly, S. (2021). Bone Morphogenetic Protein Antagonist Gremlin-1 Increases Myofibroblast Transition in Dermal Fibroblasts: Implications for Systemic Sclerosis. Frontiers in Cell and Developmental Biology, 9, Article 681061. https://doi.org/10.3389/fcell.2021.681061

Objective: Systemic Sclerosis is an autoimmune connective tissue disease which results in fibrosis of the skin and lungs. The disease is characterized by activation of myofibroblasts but what governs this is unknown. Gremlin-1 is a BMP antagonist tha... Read More about Bone Morphogenetic Protein Antagonist Gremlin-1 Increases Myofibroblast Transition in Dermal Fibroblasts: Implications for Systemic Sclerosis.

Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts (2021)
Journal Article
Smith, L., Hidalgo Aguilar, A., Owens, D., Quelch, R., Knight, E., & Przyborski, S. (2021). Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts. Frontiers in Cell and Developmental Biology, 9, https://doi.org/10.3389/fcell.2021.667246

Various methods are currently used to investigate human tissue differentiation, including human embryo culture and studies utilising pluripotent stem cells (PSCs) such as in vitro embryoid body formation and in vivo teratoma assays. Each method has i... Read More about Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts.

The vitamin A ester retinyl propionate has a unique metabolic profile and higher retinoid‐related bioactivity over retinol and retinyl palmitate in human skin models (2020)
Journal Article
Bjerke, D. L., Li, R., Price, J. M., Dobson, R. L., Rodrigues, M., Tey, C., …Oblong, J. E. (2021). The vitamin A ester retinyl propionate has a unique metabolic profile and higher retinoid‐related bioactivity over retinol and retinyl palmitate in human skin models. Experimental Dermatology, 30(2), 226-236. https://doi.org/10.1111/exd.14219

Human skin is exposed daily to environmental stressors, which cause acute damage and inflammation. Over time this leads to morphological and visual appearance changes associated with premature aging. Topical vitamin A derivatives such as retinol (ROL... Read More about The vitamin A ester retinyl propionate has a unique metabolic profile and higher retinoid‐related bioactivity over retinol and retinyl palmitate in human skin models.

microRNA27a-3p mediates reduction of the Wnt antagonist sFRP-1 in systemic sclerosis (2020)
Journal Article
Henderson, J., Wilkinson, S., Przyborski, S., Stratton, R., & O’Reilly, S. (2021). microRNA27a-3p mediates reduction of the Wnt antagonist sFRP-1 in systemic sclerosis. Epigenetics, 16(7), 808-817. https://doi.org/10.1080/15592294.2020.1827715

Systemic Sclerosis (SSc) is an autoimmune connective tissue disease that leads to skin and lung fibrosis. The Wnt pathway is clearly elevated in SSc and is pro-fibrotic via activation of canonical Wnt signalling. sFRP-1 is a Wnt antagonist that acts... Read More about microRNA27a-3p mediates reduction of the Wnt antagonist sFRP-1 in systemic sclerosis.

Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function (2020)
Journal Article
Darling, N. J., Mobbs, C. L., González-Hau, A. L., Freer, M., & Przyborski, S. (2020). Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function. Frontiers in Bioengineering and Biotechnology, 8, Article 992. https://doi.org/10.3389/fbioe.2020.00992

The Caco-2 monolayer is the most widely used in vitro model of the human intestinal mucosa to study absorption. However, models lack communication from other cells present in the native intestine, such as signals from fibroblasts in the lamina propri... Read More about Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function.

Germline TET2 loss of function causes childhood immunodeficiency and lymphoma (2020)
Journal Article
Stremenova Spegarova, J., Lawless, D., Mohamad, S. M. B., Engelhardt, K. R., Doody, G., Shrimpton, J., …Hambleton, S. (2020). Germline TET2 loss of function causes childhood immunodeficiency and lymphoma. Blood, 136(9), 1055-1066. https://doi.org/10.1182/blood.2020005844

Molecular dissection of inborn errors of immunity can help to elucidate the nonredundant functions of individual genes. We studied 3 children with an immune dysregulation syndrome of susceptibility to infection, lymphadenopathy, hepatosplenomegaly, d... Read More about Germline TET2 loss of function causes childhood immunodeficiency and lymphoma.