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All Outputs (47)

Allgemeine Methoden (2007)
Book Chapter
Frech, M., Mikosch, T., Schröder, M., & Stassen, M. (2007). Allgemeine Methoden. In M. Jansohn (Ed.), Gentechnische Methoden: eine Sammlung von Arbeitsanleitungen für das molekularbiologische Labor (1-35). (4th ed.). Elsevier

The unfolded protein response (2006)
Journal Article
Schröder, M. (2006). The unfolded protein response. Molecular Biotechnology, 34(2), 279-290

The unfolded protein response (UPR) is a signal transduction network activated by inhibition of protein folding in the endoplasmic reticulum (ER). The UPR coordinates adaptive responses to this stress situation, including induction of ER resident mol... Read More about The unfolded protein response.

Divergent roles for IRE1α and PERK in the unfolded protein response (2006)
Journal Article
Schröder, M., & Kaufman, R. (2006). Divergent roles for IRE1α and PERK in the unfolded protein response. Current Molecular Medicine, 6(1), 5-36

The endoplasmic reticulum (ER) provides unique machinery for the folding and posttranslational modification of many secretory and transmembrane proteins in eukaryotic cells. The unfolded protein response (UPR) is a signal transduction network from th... Read More about Divergent roles for IRE1α and PERK in the unfolded protein response.

The mammalian unfolded protein response (2005)
Journal Article
Schröder, M., & Kaufman, R. (2005). The mammalian unfolded protein response. Annual Review of Biochemistry, 74, 739-789

In the endoplasmic reticulum (ER) secretory and transmembrane proteins fold into their native conformation and undergo posttranslational modifications important for their activity and structure. When protein folding in the ER is inhibited, signal tra... Read More about The mammalian unfolded protein response.

ER stress signalling by regulated splicing: IRE1/HAC1/XBP1 (2005)
Journal Article
Back, S., Schröder, M., Lee, K., Zhang, K., & Kaufman, R. (2005). ER stress signalling by regulated splicing: IRE1/HAC1/XBP1. Methods, 35(4), 395-416. https://doi.org/10.1016/j.ymeth.2005.03.001

The endoplasmic reticulum (ER) serves many specialized functions in the cell including calcium storage and gated release, biosynthesis of membrane and secretory proteins, and production of lipids and sterols. Therefore, the ER integrates many interna... Read More about ER stress signalling by regulated splicing: IRE1/HAC1/XBP1.

ER stress and the unfolded protein response (2005)
Journal Article
Schröder, M., & Kaufman, R. (2005). ER stress and the unfolded protein response. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 569(1-2), 29-63. https://doi.org/10.1016/j.mrfmmm.2004.06.056

Conformational diseases are caused by mutations altering the folding pathway or final conformation of a protein. Many conformational diseases are caused by mutations in secretory proteins and reach from metabolic diseases, e.g. diabetes, to developme... Read More about ER stress and the unfolded protein response.

GermOnline, a cross-species knowledgebase on germ cell differentiation (2004)
Journal Article
Wiederkehr, C., Basavaraj, R., Sarrauste de Menthiere, C., Hermida, L., Koch, R., Schlecht, U., …Primig, M. (2004). GermOnline, a cross-species knowledgebase on germ cell differentiation. Nucleic Acids Research, 32(Database issue), D560-D567. https://doi.org/10.1093/nar/gkh055

GermOnline provides information and microarray expression data for genes involved in mitosis and meiosis, gamete formation and germ line development across species. The database has been developed, and is being curated and updated, by life scientists... Read More about GermOnline, a cross-species knowledgebase on germ cell differentiation.

The unfolded protein response represses differentiation through the RPD3-SIN3 histone deacetylase (2004)
Journal Article
Schröder, M., Clark, R., Liu, C., & Kaufman, R. (2004). The unfolded protein response represses differentiation through the RPD3-SIN3 histone deacetylase. The EMBO Journal, 23(11), 2281-2292. https://doi.org/10.1038/sj.emboj.7600233

In Saccharomyces cerevisiae, splicing of HAC1 mRNA is initiated in response to the accumulation of unfolded proteins in the endoplasmic reticulum by the transmembrane kinase-endoribonuclease Ire1p. Spliced Hac1p (Hac1ip) is a negative regulator of di... Read More about The unfolded protein response represses differentiation through the RPD3-SIN3 histone deacetylase.

Serum- and protein-free media formulations for the Chinese hamster ovary cell line DUKXB11 (2004)
Journal Article
Schröder, M., Matischak, K., & Friedl, P. (2004). Serum- and protein-free media formulations for the Chinese hamster ovary cell line DUKXB11. Journal of Biotechnology, 108(3), 279-292. https://doi.org/10.1016/j.jbiotec.2003.12.005

The production of therapeutic proteins in mammalian cell lines is of outstanding importance. The maintenance of most mammalian cell lines in culture requires the addition of serum to the culture medium. The elimination of serum from mammalian cell cu... Read More about Serum- and protein-free media formulations for the Chinese hamster ovary cell line DUKXB11.

IRE1- and HAC1-independent transcriptional regulation in the unfolded protein response of yeast (2003)
Journal Article
Schröder, M., Clark, R., & Kaufman, R. (2003). IRE1- and HAC1-independent transcriptional regulation in the unfolded protein response of yeast. Molecular Microbiology, 49(3), 591-606. https://doi.org/10.1046/j.1365-2958.2003.03585.x

The unfolded protein response (UPR) is a signalling pathway leading to transcriptional activation of genes that protect cells from accumulation of unfolded proteins in the lumen of the endoplasmic reticulum (ER). In yeast, the only known ER stress si... Read More about IRE1- and HAC1-independent transcriptional regulation in the unfolded protein response of yeast.

Isolierung von RNA (2002)
Book Chapter
Schröder, M. (2002). Isolierung von RNA. In G. Schrimpf (Ed.), Gentechnische Methoden (129-145). (3). Spektrum Academischer Verlag

Allgemeine Methoden (2002)
Book Chapter
Frech, M., Mikosch, T., Schröder, M., & Stassen, M. (2002). Allgemeine Methoden. In G. Schrimpf, & A. Aigner (Eds.), Gentechnische Methoden: eine Sammlung von Arbeitsanleitungen für das molekularbiologische Labor (1-37). (3rd ed.). Spektrum Akademischer Verlag

The unfolded protein response in nutrient sensing and differentiation (2002)
Journal Article
Kaufman, R., Scheuner, D., Schröder, M., Shen, X., Lee, K., Liu, C., & Arnold, S. (2002). The unfolded protein response in nutrient sensing and differentiation. Nature Reviews Molecular Cell Biology, 3(6), 411-421. https://doi.org/10.1038/nrm829

Eukaryotic cells coordinate protein-folding reactions in the endoplasmic reticulum with gene expression in the nucleus and messenger RNA translation in the cytoplasm. As the rate of protein synthesis increases, protein folding can be compromised, so... Read More about The unfolded protein response in nutrient sensing and differentiation.

Induction of protein aggregation in an early secretory compartment by elevation of expression level (2002)
Journal Article
Schröder, M., Schäfer, R., & Friedl, P. (2002). Induction of protein aggregation in an early secretory compartment by elevation of expression level. Biotechnology and Bioengineering, 78(2), 131-140. https://doi.org/10.1002/bit.10206

A variety of valuable therapeutic proteins are expressed in mammalian cells. Currently, rate-limiting for secretion of recombinant glycoproteins are activities in the secretory pathway of eukaryotic cells, i.e., folding and glycosylation of the naked... Read More about Induction of protein aggregation in an early secretory compartment by elevation of expression level.

The unfolded protein response represses nitrogen-starvation induced developmental differentiation in yeast (2000)
Journal Article
Schröder, M., Chang, J., & Kaufman, R. (2000). The unfolded protein response represses nitrogen-starvation induced developmental differentiation in yeast. Genes & Development, 14(23), 2962-2975

Diploid budding yeast exhibits two developmental programs in response to nitrogen starvation, pseudohyphal growth, and sporulation. Here we show that both programs are repressed by activation of the unfolded protein response (UPR), a stress-signal tr... Read More about The unfolded protein response represses nitrogen-starvation induced developmental differentiation in yeast.

Ligand-independent dimerization activates the stress response kinases IRE1 and PERK in the lumen of the endoplasmic reticulum (2000)
Journal Article
Liu, C., Schröder, M., & Kaufman, R. (2000). Ligand-independent dimerization activates the stress response kinases IRE1 and PERK in the lumen of the endoplasmic reticulum. Journal of Biological Chemistry, 275(32), 24881-24885

IRE1 and PERK are type I transmembrane serine/threonine protein kinases that are activated by unfolded proteins in the endoplasmic reticulum (ER) to signal adaptive responses. IRE1 is present in all eukaryotic cells and signals the unfolded protein r... Read More about Ligand-independent dimerization activates the stress response kinases IRE1 and PERK in the lumen of the endoplasmic reticulum.

Quantitative analysis of transcription and translation in gene amplified Chinese hamster ovary cells on the basis of a kinetic model (1999)
Journal Article
Schröder, M., Körner, C., & Friedl, P. (1999). Quantitative analysis of transcription and translation in gene amplified Chinese hamster ovary cells on the basis of a kinetic model. Cytotechnology, 29(2), 93-102. https://doi.org/10.1023/a%3A1008077603328

The elevation of expression levels for secreted glycoproteins by gene amplification in mammalian cells shows a saturation behavior at high levels of gene amplification. At high expression levels a drop in the secretion efficiency for the recombinant... Read More about Quantitative analysis of transcription and translation in gene amplified Chinese hamster ovary cells on the basis of a kinetic model.

Isolierung von RNA (1999)
Book Chapter
Schröder, M. (1999). Isolierung von RNA. In H. Gassen, & G. Schrimpf (Eds.), Genrechnische Methoden (225-242). (2). Spektrum Akademischer Verlag

Enzymatic determination of D-glucose in cell culture media using glucose oxidase, horseradish peroxidase and 3,3 ',5,5 '- tetramethylbenzidine (1997)
Journal Article
Schröder, M., & Friedl, P. (1997). Enzymatic determination of D-glucose in cell culture media using glucose oxidase, horseradish peroxidase and 3,3 ',5,5 '- tetramethylbenzidine

An enzymatic assay for the determination of D-glucose using glucose oxidase, horseradishperoxidase (HRP) and 3,3',5,5'-tetramethylbenzidine (TMB) in cell culture media containing bovine serum is described. The major advantages of this assay compared... Read More about Enzymatic determination of D-glucose in cell culture media using glucose oxidase, horseradish peroxidase and 3,3 ',5,5 '- tetramethylbenzidine.