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All Outputs (93)

A dynamin-actin interaction is required for vesicle scission during endocytosis in yeast (2015)
Journal Article
Palmer, S., Smaczynska-de Rooij, I., Marklew, C., Allwood, E., Mishra, R., Johnson, S., …Ayscough, K. (2015). A dynamin-actin interaction is required for vesicle scission during endocytosis in yeast. Current Biology, 25(7), 868-878. https://doi.org/10.1016/j.cub.2015.01.061

Actin is critical for endocytosis in yeast cells, and also in mammalian cells under tension. However, questions remain as to how force generated through actin polymerization is transmitted to the plasma membrane to drive invagination and scission. He... Read More about A dynamin-actin interaction is required for vesicle scission during endocytosis in yeast.

Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM (2014)
Book Chapter
Kiseleva, E., Richardson, A. C., Fiserova, J., Strunov, A. A., Spink, M. C., Johnson, S. R., & Goldberg, M. W. (2014). Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM. In Nuclear Pore Complexes and Nucleocytoplasmic Transport - Methods (59-79). Elsevier/Academic Press. https://doi.org/10.1016/b978-0-12-417160-2.00003-5

Electron microscopy (EM) has been used extensively for the study of nuclear transport as well as the structure of the nuclear pore complex (NPC) and nuclear envelope. However, there are specific challenges faced when carrying out EM in one of the mai... Read More about Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM.

A gradient of matrix-bound FGF-2 and perlecan is available to lens epithelial cells (2014)
Journal Article
Wu, W., Tholozan, F., Goldberg, M., Bowen, L., Wu, J., & Quinlan, R. (2014). A gradient of matrix-bound FGF-2 and perlecan is available to lens epithelial cells. Experimental Eye Research, 120, 10-14. https://doi.org/10.1016/j.exer.2013.12.004

Fibroblast growth factors play a key role in regulating lens epithelial cell proliferation and differentiation via an anteroposterior gradient that exists between the aqueous and vitreous humours. FGF-2 is the most important for lens epithelial cell... Read More about A gradient of matrix-bound FGF-2 and perlecan is available to lens epithelial cells.

Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains (2014)
Journal Article
Fiserova, J., Spink, M., Richards, S., Saunter, C., & Goldberg, M. (2014). Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains. Journal of Cell Science, 127, 124-136. https://doi.org/10.1242/jcs.133272

Nuclear pore complexes (NPCs) mediate nucleocytoplasmic movement. The central channel contains proteins with phenylalanine-glycine (FG) repeats, or variations (GLFG, glycine-leucine-phenylalanine-glycine). These are ‘intrinsically disordered’ and oft... Read More about Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains.

Microscopic visualisation of metabotropic glutamate receptors on the surface of living cells using bifunctional magnetic resonance imaging probes (2013)
Other
Mishra, A., Mishra, R., Gottschalk, S., Pal, R., Sim, N., Engelmann, J., …Parker, D. (2014). Microscopic visualisation of metabotropic glutamate receptors on the surface of living cells using bifunctional magnetic resonance imaging probes

A series of bimodal metabotropic glutamate-receptor targeted MRI contrast agents has been developed and evaluated, based on established competitive metabotropic Glu receptor subtype 5 (mGluR5) antagonists. In order to directly visualize mGluR5 bindin... Read More about Microscopic visualisation of metabotropic glutamate receptors on the surface of living cells using bifunctional magnetic resonance imaging probes.

Nesprin interchain associations control nuclear size. (2012)
Journal Article
Lu, W., Schneider, M., Neumann, S., Jaeger, V., Taranum, S., Munck, M., …Karakesisoglou, I. (2012). Nesprin interchain associations control nuclear size. Cellular and Molecular Life Sciences, 69(20), 3493-3509. https://doi.org/10.1007/s00018-012-1034-1

Nesprins-1/-2/-3/-4 are nuclear envelope proteins, which connect nuclei to the cytoskeleton. The largest nesprin-1/-2 isoforms (termed giant) tether F-actin through their N-terminal actin binding domain (ABD). Nesprin-3, however, lacks an ABD and ass... Read More about Nesprin interchain associations control nuclear size..

Yeast dynamin Vps1 and amphiphysin Rvs167 function together during endocytosis (2012)
Journal Article
Rooij, I. I. S., Allwood, E. G., Mishra, R., Booth, W. I., Aghamohammadzadeh, S., Goldberg, M. W., & Ayscough, K. R. (2012). Yeast dynamin Vps1 and amphiphysin Rvs167 function together during endocytosis. Traffic, 13(2), 317-328. https://doi.org/10.1111/j.1600-0854.2011.01311.x

Dynamins are a conserved family of proteins involved in many membrane fusion and fission events. Previously, the dynamin-related protein Vps1 was shown to localize to endocytic sites, and yeast carrying deletions for genes encoding both the BAR domai... Read More about Yeast dynamin Vps1 and amphiphysin Rvs167 function together during endocytosis.

Embryonic and adult isoforms of XLAP2 form microdomains associated with chromatin and the nuclear envelope (2011)
Journal Article
Chmielewska, M., Dubińska-Magiera, M., Sopel, M., Rzepecka, D., Hutchison, C., Goldberg, M., & Rzepecki, R. (2011). Embryonic and adult isoforms of XLAP2 form microdomains associated with chromatin and the nuclear envelope. Cell and Tissue Research, 344(1), 97-110. https://doi.org/10.1007/s00441-011-1129-2

Laminin-associated polypeptide 2 (LAP2) proteins are alternatively spliced products of a single gene; they belong to the LEM domain family and, in mammals, locate to the nuclear envelope (NE) and nuclear lamina. Isoforms lacking the transmembrane dom... Read More about Embryonic and adult isoforms of XLAP2 form microdomains associated with chromatin and the nuclear envelope.

System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics (2011)
Journal Article
Zuleger, N., Kelly, D. A., Richardson, A. C., Kerr, A. R., Goldberg, M. W., Goryachev, A. B., & Schirmer, E. C. (2011). System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics. Journal of Cell Biology, 193(1), 109-123. https://doi.org/10.1083/jcb.201009068

The nuclear envelope contains >100 transmembrane proteins that continuously exchange with the endoplasmic reticulum and move within the nuclear membranes. To better understand the organization and dynamics of this system, we compared the trafficking... Read More about System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics.

Expression of Vps1 I649K a self-assembly defective yeast dynamin, leads to formation of extended endocytic invaginations. (2011)
Journal Article
Mishra, R., Smaczynska-de Rooij, I., Goldberg, M., & Ayscough, K. (2011). Expression of Vps1 I649K a self-assembly defective yeast dynamin, leads to formation of extended endocytic invaginations. Communicative and Integrative Biology, 4(1), 115-117. https://doi.org/10.4161/cib.14206

The dynamin proteins have been associated with the process of endocytosis for many years. Until recently it was considered that yeast dynamin-related proteins did not play a role in endocytosis and the proposed scission function of dynamin was attrib... Read More about Expression of Vps1 I649K a self-assembly defective yeast dynamin, leads to formation of extended endocytic invaginations..

A role for the dynamin-like protein Vps1 during endocytosis in yeast. (2010)
Journal Article
Smaczynska-de Rooij, I., Allwood, E., Aghamohammadzadeh, S., Hettema, E., Goldberg, M., & Ayscough, K. (2010). A role for the dynamin-like protein Vps1 during endocytosis in yeast. Journal of Cell Science, 123(20), 3496-3506. https://doi.org/10.1242/%26%238203%3Bjcs.070508

Dynamins are a conserved family of proteins involved in membrane fusion and fission. Although mammalian dynamins are known to be involved in several membrane-trafficking events, the role of dynamin-1 in endocytosis is the best-characterised role of t... Read More about A role for the dynamin-like protein Vps1 during endocytosis in yeast..