Microscopic visualisation of metabotropic glutamate receptors on the surface of living cells using bifunctional magnetic resonance imaging probes

Mishra, Anurag; Mishra, Rita; Gottschalk, Sven; Pal, Robert; Sim, Neil; Engelmann, Joern; Goldberg, Martin; Parker, David

Abstract

A series of bimodal metabotropic glutamate-receptor targeted MRI contrast agents has been developed and evaluated, based on established competitive metabotropic Glu receptor subtype 5 (mGluR5) antagonists. In order to directly visualize mGluR5 binding of these agents on the surface of live astrocytes, variations in the core structure were made. A set of gadolinium conjugates containing either a cyanine dye or a fluorescein moiety was accordingly prepared, to allow visualization by optical microscopy in cellulo. In each case, surface receptor binding was compromised and cell internalization observed. Another approach, examining the location of a terbium analogue via sensitized emission, also exhibited nonspecific cell uptake in neuronal cell line models. Finally, biotin derivatives of two lead compounds were prepared, and the specificity of binding to the mGluR5 cell surface receptors was demonstrated with the aid of their fluorescently labeled avidin conjugates, using both total internal reflection fluorescence (TIRF) and confocal microscopy.

Citation

Mishra, A., Mishra, R., Gottschalk, S., Pal, R., Sim, N., Engelmann, J., …Parker, D. (2014). Microscopic visualisation of metabotropic glutamate receptors on the surface of living cells using bifunctional magnetic resonance imaging probes

• Other Type: Other
• Online Publication Date: Nov 19, 2013
• Publication Date: Feb 19, 2014
• Deposit Date: Jun 19, 2014
• Publicly Available Date: Jul 15, 2014
• Pages: 128-137
• DOI: https://doi.org/10.1021/cn400175m
• Keywords: mGluR5, Imaging agents, Lanthanides, MRI, Microscopy.
• Public URL: https://durham-repository.worktribe.com/output/1646612
• Additional Information: URL of output: https://doi.org/10.1021/cn400175m; Volume: 5; Issue: 2; ISSN or ISBN: 1948-7193