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The crystal structures of apo and cAMP-bound GlxR from Corynebacterium glutamicum reveal structural and dynamic changes upon cAMP binding in CRP/FNR family transcription factors (2014)
Journal Article
Townsend, P., Jungwirth, B., Pojer, P., Bußmann, M., Money, V., Cole, S., …Pohl, E. (2014). The crystal structures of apo and cAMP-bound GlxR from Corynebacterium glutamicum reveal structural and dynamic changes upon cAMP binding in CRP/FNR family transcription factors. PLoS ONE, 9(12), Article e113265. https://doi.org/10.1371/journal.pone.0113265

The cyclic AMP-dependent transcriptional regulator GlxR from Corynebacterium glutamicum is a member of the super-family of CRP/FNR (cyclic AMP receptor protein/fumarate and nitrate reduction regulator) transcriptional regulators that play central rol... Read More about The crystal structures of apo and cAMP-bound GlxR from Corynebacterium glutamicum reveal structural and dynamic changes upon cAMP binding in CRP/FNR family transcription factors.

CO2 directly modulates connexin 26 by formation of carbamate bridges between subunits (2013)
Journal Article
Meigh, L., Greenhalgh, S., Rodgers, T., Cann, M., Roper, D., & Dale, N. (2013). CO2 directly modulates connexin 26 by formation of carbamate bridges between subunits. eLife, 2, https://doi.org/10.7554/elife.01213

Homeostatic regulation of the partial pressure of CO2 (PCO2) is vital for life. Sensing of pH has been proposed as a sufficient proxy for determination of PCO2 and direct CO2-sensing largely discounted. Here we show that connexin 26 (Cx26) hemichanne... Read More about CO2 directly modulates connexin 26 by formation of carbamate bridges between subunits.

Modulation of Global Low-Frequency Motions Underlies Allosteric Regulation: Demonstration in CRP/FNR Family Transcription Factors (2013)
Journal Article
Rodgers, T., Townsend, P., Burnell, D., Jones, M., Richards, S., McLeish, T., …Cann, M. (2013). Modulation of Global Low-Frequency Motions Underlies Allosteric Regulation: Demonstration in CRP/FNR Family Transcription Factors. PLoS Biology, 11(9), Article e1001651. https://doi.org/10.1371/journal.pbio.1001651

Allostery is a fundamental process by which ligand binding to a protein alters its activity at a distinct site. There is growing evidence that allosteric cooperativity can be communicated by modulation of protein dynamics without conformational chang... Read More about Modulation of Global Low-Frequency Motions Underlies Allosteric Regulation: Demonstration in CRP/FNR Family Transcription Factors.

Elevated Carbon Dioxide Blunts Mammalian cAMP Signaling Dependent on Inositol 1,4,5-Triphosphate Receptor-mediated Ca2+ Release (2012)
Journal Article
Cook, Z., Gray, M., & Cann, M. (2012). Elevated Carbon Dioxide Blunts Mammalian cAMP Signaling Dependent on Inositol 1,4,5-Triphosphate Receptor-mediated Ca2+ Release. Journal of Biological Chemistry, 287(31), 26291-26301. https://doi.org/10.1074/jbc.m112.349191

Elevated CO2 is generally detrimental to animal cells, suggesting an interaction with core processes in cell biology. We demonstrate that elevated CO2 blunts G protein-activated cAMP signaling. The effect of CO2 is independent of changes in intracell... Read More about Elevated Carbon Dioxide Blunts Mammalian cAMP Signaling Dependent on Inositol 1,4,5-Triphosphate Receptor-mediated Ca2+ Release.

A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice (2012)
Journal Article
Fenyk, S., de San Eustaquio Campillo, A., Pohl, E., Hussey, P., & Cann, M. (2012). A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice. Journal of Biological Chemistry, 287(6), 4023-4032. https://doi.org/10.1074/jbc.m111.314450

Plant resistance proteins (R-proteins) are key components of the plant immune system activated in response to a plethora of different pathogens. R-proteins are P-loop NTPase superfamily members, and current models describe their main function as ATPa... Read More about A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice.

The Pseudomonas aeruginosa Chp chemosensory system regulates intracellular cAMP levels by modulating adenylate cyclase activity (2010)
Journal Article
Fulcher, N., Holliday, P., Klem, E., Cann, M., & Wolfgang, M. (2010). The Pseudomonas aeruginosa Chp chemosensory system regulates intracellular cAMP levels by modulating adenylate cyclase activity. Molecular Microbiology, 76(4), 889-904. https://doi.org/10.1111/j.1365-2958.2010.07135.x

Multiple virulence systems in the opportunistic pathogen Pseudomonas aeruginosa are regulated by the second messenger signalling molecule adenosine 3′, 5′-cyclic monophosphate (cAMP). Production of cAMP by the putative adenylate cyclase enzyme CyaB r... Read More about The Pseudomonas aeruginosa Chp chemosensory system regulates intracellular cAMP levels by modulating adenylate cyclase activity.

Stimulation of Mammalian G-protein-responsive Adenylyl Cyclases by Carbon Dioxide (2009)
Journal Article
Townsend, P., Holliday, P., Fenyk, S., Hess, K., Gray, M., Hodgson, D., & Cann, M. (2009). Stimulation of Mammalian G-protein-responsive Adenylyl Cyclases by Carbon Dioxide. Journal of Biological Chemistry, 284(2), 784-791. https://doi.org/10.1074/jbc.m807239200

Carbon dioxide is fundamental to the physiology of all organisms. There is considerable interest in the precise molecular mechanisms that organisms use to directly sense CO2. Here we demonstrate that a mammalian recombinant G-protein-activated adenyl... Read More about Stimulation of Mammalian G-protein-responsive Adenylyl Cyclases by Carbon Dioxide.

Sodium regulation of GAF domain function (2007)
Journal Article
Cann, M. (2007). Sodium regulation of GAF domain function. Biochemical Society Transactions, 35(5), 1032-1034. https://doi.org/10.1042/bst0351032

Cyclic nucleotide PDEs (phosphodiesterases) regulate cellular levels of cAMP and cGMP by controlling the rate of degradation. Several mammalian PDE isoforms possess N-terminal GAF (found in cGMP PDEs, Anabaena adenylate cyclases and Escherichia coli... Read More about Sodium regulation of GAF domain function.

Synthesis of 5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate and its Enzymatic Incorporation at the 5'-Termini of RNA Molecules (2007)
Journal Article
Williamson, D., Cann, M., & Hodgson, D. (2007). Synthesis of 5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate and its Enzymatic Incorporation at the 5'-Termini of RNA Molecules. Chemical Communications, 2007(47), 5096-5098. https://doi.org/10.1039/b712066d

5-Amino-5-deoxyguanosine-5-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5-H2N-RNA that can be conjugated to activated esters.

A subset of GAF domains are evolutionarily conserved sodium sensors (2007)
Journal Article
Cann, M. (2007). A subset of GAF domains are evolutionarily conserved sodium sensors. Molecular Microbiology, 64(2), 461-472. https://doi.org/10.1111/j.1365-2958.2007.05669.x

Most organisms maintain a transmembrane sodium gradient for cell function. Despite the importance of Na+ in physiology, no directly Na+-responsive signalling molecules are known. The CyaB1 and CyaB2 adenylyl cyclases of the cyanobacterium Anabaena PC... Read More about A subset of GAF domains are evolutionarily conserved sodium sensors.

A europium complex that selectively stains nucleoli of cells (2006)
Journal Article
Yu, J., Parker, D., Pal, R., Poole, R., & Cann, M. (2006). A europium complex that selectively stains nucleoli of cells. Journal of the American Chemical Society, 128(7), 2294-2299. https://doi.org/10.1021/ja056303g

A europium complex selectively staining the nucleolus of NIH 3T3, HeLa, and HDF cells is reported. This complex possesses not only the advantage of the long lifetime of europium emission (0.3 ms), but also a chromophore that allows excitation at a re... Read More about A europium complex that selectively stains nucleoli of cells.

Regulation of prokaryotic adenylyl cyclases by CO₂ (2006)
Journal Article
Hammer, A., Hodgson, D., & Cann, M. (2006). Regulation of prokaryotic adenylyl cyclases by CO₂. Biochemical Journal, 396(2), 215-218. https://doi.org/10.1042/bj20060372

The Slr1991 adenylyl cyclase of the model prokaroyte Synechocystis PCC 6803 was stimulated 2-fold at 20 mM total Ci (inorganic carbon) atpH 7.5 through an increase in kcat.Adose response demonstrated an EC50 of 52.7 mMtotal Ci at pH 6.5. Slr1991 aden... Read More about Regulation of prokaryotic adenylyl cyclases by CO₂.

Synthesis and characterisation of highly emissive and kinetically stable lanthanide complexes suitable for usage 'in cellulo' (2005)
Journal Article
Poole, R., Bobba, G., Cann, M., Frias, J., Parker, D., & Peacock, R. (2005). Synthesis and characterisation of highly emissive and kinetically stable lanthanide complexes suitable for usage 'in cellulo'. Organic and Biomolecular Chemistry, 3(6), 1013-1024. https://doi.org/10.1039/b418964g

The synthesis and photophysical characterisation are reported of a series of cationic, neutral and anionic europium and terbium complexes based on structurally related, nonadentate ligands based on the cyclen macrocycle. Each complex incorporates a t... Read More about Synthesis and characterisation of highly emissive and kinetically stable lanthanide complexes suitable for usage 'in cellulo'.

Bicarbonate stimulated adenylyl cyclases (2004)
Journal Article
Cann, M. (2004). Bicarbonate stimulated adenylyl cyclases. IUBMB Life, 56(9), 529-534. https://doi.org/10.1080/15216540400013861

Bicarbonate ion is fundamental to the biology of all living organisms. HCO(3)(-) is vital to such diverse physiological processes as carbon fixation, cellular homeostasis, sperm maturation, and nucleotide synthesis. A defined subset of adenylyl cycla... Read More about Bicarbonate stimulated adenylyl cyclases.

Design, synthesis and evaluation of ratiometric probes for hydrogencarbonate based on europium emission (2004)
Journal Article
Bretonniere, Y., Cann, M., Parker, D., & Slater, R. (2004). Design, synthesis and evaluation of ratiometric probes for hydrogencarbonate based on europium emission. Organic and Biomolecular Chemistry, 2(11), 1624-1632. https://doi.org/10.1039/b400734b

A series of cationic, zwitterionic and anionic macrocyclic europium complexes has been prepared incorporating a N or C- linked acridone chromophore that allows sensitisation of Eu emission following excitation at 390-410nm. Each of these complexes se... Read More about Design, synthesis and evaluation of ratiometric probes for hydrogencarbonate based on europium emission.

Luminescent nonacoordinate cationic lanthanide complexes as potential cellular imaging and reactive probes (2003)
Journal Article
Frias, J., Bobba, G., Cann, M., Hutchison, C., & Parker, D. (2003). Luminescent nonacoordinate cationic lanthanide complexes as potential cellular imaging and reactive probes. Organic and Biomolecular Chemistry, 1(6), 905-907

Nonacoordinate Delta- and Lambda-Eu and Tb complexes have been tested as imaging and reactive probes in mouse fibroblast (NIH 3T3) cells. The uptake of these complexes by the cells was assessed by fluorescence microscopy. Complex-induced DNA damage w... Read More about Luminescent nonacoordinate cationic lanthanide complexes as potential cellular imaging and reactive probes.

A defined subset of adenylyl cyclases is regulated by bicarbonate ion (2003)
Journal Article
Cann, M., Hammer, A., Zhou, J., & Kanacher, T. (2003). A defined subset of adenylyl cyclases is regulated by bicarbonate ion. Journal of Biological Chemistry, 278(37), 35033-35038. https://doi.org/10.1074/jbc.m303025200

The molecular basis by which organisms detect and respond to fluctuations in inorganic carbon is not known. The cyaB1 gene of the cyanobacterium Anabaena sp. PCC7120 codes for a multidomain protein with a C-terminal class III adenylyl cyclase catalys... Read More about A defined subset of adenylyl cyclases is regulated by bicarbonate ion.