All Outputs (22)

Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome (2022)
Journal Article
Guillen-Garcia, A., Gibson, S., Jordan, C., Ramaswamy, V., Linthwaite, V., Bromley, E., …Cann, M. (2022). Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome. Nature Communications, 13, Article 5289. https://doi.org/10.1038/s41467-022-32925-6

Light harvesting is fundamental for production of ATP and reducing equivalents for CO2 fixation during photosynthesis. However, electronic energy transfer (EET) through a photosystem can harm the photosynthetic apparatus when not balanced with CO2. H... Read More about Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome.

Ubiquitin is a carbon dioxide-binding protein (2021)
Journal Article
Linthwaite, V., Pawloski, W., Pegg, H., Townsend, P., Thomas, M., Brown, A., …Cann, M. (2021). Ubiquitin is a carbon dioxide-binding protein. Science Advances, 7(39), Article eabi5507. https://doi.org/10.1126/sciadv.abi5507

The identification of CO2-binding proteins is crucial to understanding CO2-regulated molecular processes. CO2 can form a reversible posttranslational modification through carbamylation of neutral N-terminal -amino or lysine -amino groups. We have p... Read More about Ubiquitin is a carbon dioxide-binding protein.

Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake (2021)
Journal Article
Smith, P. O., Black, D. J., Pal, R., Avó, J., Dias, F. B., Linthwaite, V. L., …Pålsson, L. (2021). Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake. Frontiers in Chemistry, 9, Article 743928. https://doi.org/10.3389/fchem.2021.743928

A new method for facilitating the delivery, uptake and intracellular localisation of thermally activated delayed fluorescence (TADF) complexes was developed. First, confinement of TADF complexes in liposomes was demonstrated, which were subsequently... Read More about Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake.

The identification of carbon dioxide mediated protein post-translational modifications (2018)
Journal Article
Linthwaite, V., Janus, J., Brown, A., Wong-Pascua, D., O’Donoghue, A., Porter, A., …Cann, M. (2018). The identification of carbon dioxide mediated protein post-translational modifications. Nature Communications, 9, Article 3092. https://doi.org/10.1038/s41467-018-05475-z

Carbon dioxide is vital to the chemistry of life processes including metabolism, cellular homoeostasis, and pathogenesis. CO2 is generally unreactive but can combine with neutral amines to form carbamates on proteins under physiological conditions. T... Read More about The identification of carbon dioxide mediated protein post-translational modifications.

The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor (2017)
Journal Article
Townsend, P., Dixon, C., Slootweg, E., Sukarta, O., Yang, A., Hughes, T., …Cann, M. (2018). The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor. Journal of Biological Chemistry, 293(9), 3218-3233. https://doi.org/10.1074/jbc.ra117.000485

Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming and some NLRs have been shown to act in the nucleus and interact with transcription fa... Read More about The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor.

The tomato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor I-2 couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange (2015)
Journal Article
Fenyk, S., Dixon, C. H., Kittens, W. H., Townsend, P. D., Sharples, G. .., Pålsson, L. .-O., Takken, F. L. W., & Cann, M. J. (2016). The tomato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor I-2 couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange. Journal of Biological Chemistry, 291(3), 1137-1147. https://doi.org/10.1074/jbc.m115.698589

Plant nucleotide-binding leucine-rich repeat (NLR) proteins enable plants to recognise and respond to pathogen attack. Previously, we demonstrated that the Rx1 NLR of potato is able to bind and bend DNA in vitro. DNA binding in situ requires its genu... Read More about The tomato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor I-2 couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange.

Multi-scale Approaches to Dynamical Transmission of Protein Allostery (2015)
Presentation / Conference Contribution
Townsend, P., Rodgers, T., Pohl, E., Wilson, M., Cann, M., & McLeish, T. (2015). Multi-scale Approaches to Dynamical Transmission of Protein Allostery. In L. Olivares-Quiroz, O. Guzmán-López, & H. E. Jardón-Valadez (Eds.), Physical Biology of Proteins and Peptides: Theory, Experiment, and Simulation (141-152). https://doi.org/10.1007/978-3-319-21687-4_8

We review the idea that allosteric interactions can be transmitted not by structural switching but by the more subtle route of modulation of the amplitude of thermally-activated global dynamical modes in allosteric proteins. The effect is naturally a... Read More about Multi-scale Approaches to Dynamical Transmission of Protein Allostery.

Dynamic Transmission of Protein Allostery without Structural Change: Spatial Pathways or Global Modes? (2015)
Journal Article
McLeish, T., Cann, M., & Rodgers, T. (2015). Dynamic Transmission of Protein Allostery without Structural Change: Spatial Pathways or Global Modes?. Biophysical Journal, 109(6), 1240-1250. https://doi.org/10.1016/j.bpj.2015.08.009

We examine the contrast between mechanisms for allosteric signaling that involve structural change, and those that do not, from the perspective of allosteric pathways. In particular we treat in detail the case of fluctuation-allostery by which amplit... Read More about Dynamic Transmission of Protein Allostery without Structural Change: Spatial Pathways or Global Modes?.

The Potato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor Rx1 is a Pathogen Dependent DNA-Deforming Protein (2015)
Journal Article
Fenyk, S., Townsend, P. D., Dixon, C. H., Spies, G. B., de San Eustaquio Campillo, A., Slootweg, E. J., …Cann, M. J. (2015). The Potato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor Rx1 is a Pathogen Dependent DNA-Deforming Protein. Journal of Biological Chemistry, 290(41), 24945-24960. https://doi.org/10.1074/jbc.m115.672121

Plant NLR proteins enable cells to respond to pathogen attack. Several NLRs act in the nucleus, however, conserved nuclear targets that support their role in immunity are unknown. Previously we noted a structural homology between the NB domain of NLR... Read More about The Potato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor Rx1 is a Pathogen Dependent DNA-Deforming Protein.

The role of protein-ligand contacts in allosteric regulation of the Escherichia coli Catabolite Activator Protein (2015)
Journal Article
Townsend, P., Rodgers, T., Glover, L., Korhonen, H., Richards, S., Colwell, L., …Cann, M. (2015). The role of protein-ligand contacts in allosteric regulation of the Escherichia coli Catabolite Activator Protein. Journal of Biological Chemistry, 290(36), 22225-22235. https://doi.org/10.1074/jbc.m115.669267

Allostery is a fundamental process by which ligand binding to a protein alters its activity at a distant site. Both experimental and theoretical evidence demonstrate that allostery can be communicated through altered slow relaxation protein dynamics... Read More about The role of protein-ligand contacts in allosteric regulation of the Escherichia coli Catabolite Activator Protein.

Global low-frequency motions in protein allostery: CAP as a model system (2015)
Journal Article
Townsend, P., Rogers, T., Pohl, E., Wilson, M., McLeish, T., & Cann, M. (2015). Global low-frequency motions in protein allostery: CAP as a model system. Biophysical Reviews, 7(2), 175-182. https://doi.org/10.1007/s12551-015-0163-9

Allostery is a fundamental process by which ligand binding to a protein alters its activity at a distant site. There is considerable evidence that allosteric cooperativity can be communicated by the modulation of protein dynamics without conformation... Read More about Global low-frequency motions in protein allostery: CAP as a model system.

The crystal structures of apo and cAMP-bound GlxR from Corynebacterium glutamicum reveal structural and dynamic changes upon cAMP binding in CRP/FNR family transcription factors (2014)
Journal Article
Townsend, P., Jungwirth, B., Pojer, P., Bußmann, M., Money, V., Cole, S., …Pohl, E. (2014). The crystal structures of apo and cAMP-bound GlxR from Corynebacterium glutamicum reveal structural and dynamic changes upon cAMP binding in CRP/FNR family transcription factors. PLoS ONE, 9(12), Article e113265. https://doi.org/10.1371/journal.pone.0113265

The cyclic AMP-dependent transcriptional regulator GlxR from Corynebacterium glutamicum is a member of the super-family of CRP/FNR (cyclic AMP receptor protein/fumarate and nitrate reduction regulator) transcriptional regulators that play central rol... Read More about The crystal structures of apo and cAMP-bound GlxR from Corynebacterium glutamicum reveal structural and dynamic changes upon cAMP binding in CRP/FNR family transcription factors.

Modulation of Global Low-Frequency Motions Underlies Allosteric Regulation: Demonstration in CRP/FNR Family Transcription Factors (2013)
Journal Article
Rodgers, T., Townsend, P., Burnell, D., Jones, M., Richards, S., McLeish, T., …Cann, M. (2013). Modulation of Global Low-Frequency Motions Underlies Allosteric Regulation: Demonstration in CRP/FNR Family Transcription Factors. PLoS Biology, 11(9), Article e1001651. https://doi.org/10.1371/journal.pbio.1001651

Allostery is a fundamental process by which ligand binding to a protein alters its activity at a distinct site. There is growing evidence that allosteric cooperativity can be communicated by modulation of protein dynamics without conformational chang... Read More about Modulation of Global Low-Frequency Motions Underlies Allosteric Regulation: Demonstration in CRP/FNR Family Transcription Factors.

A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice (2012)
Journal Article
Fenyk, S., de San Eustaquio Campillo, A., Pohl, E., Hussey, P., & Cann, M. (2012). A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice. Journal of Biological Chemistry, 287(6), 4023-4032. https://doi.org/10.1074/jbc.m111.314450

Plant resistance proteins (R-proteins) are key components of the plant immune system activated in response to a plethora of different pathogens. R-proteins are P-loop NTPase superfamily members, and current models describe their main function as ATPa... Read More about A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice.

Stimulation of Mammalian G-protein-responsive Adenylyl Cyclases by Carbon Dioxide (2009)
Journal Article
Townsend, P., Holliday, P., Fenyk, S., Hess, K., Gray, M., Hodgson, D., & Cann, M. (2009). Stimulation of Mammalian G-protein-responsive Adenylyl Cyclases by Carbon Dioxide. Journal of Biological Chemistry, 284(2), 784-791. https://doi.org/10.1074/jbc.m807239200

Carbon dioxide is fundamental to the physiology of all organisms. There is considerable interest in the precise molecular mechanisms that organisms use to directly sense CO2. Here we demonstrate that a mammalian recombinant G-protein-activated adenyl... Read More about Stimulation of Mammalian G-protein-responsive Adenylyl Cyclases by Carbon Dioxide.

Synthesis of 5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate and its Enzymatic Incorporation at the 5'-Termini of RNA Molecules (2007)
Journal Article
Williamson, D., Cann, M., & Hodgson, D. (2007). Synthesis of 5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate and its Enzymatic Incorporation at the 5'-Termini of RNA Molecules. Chemical Communications, 2007(47), 5096-5098. https://doi.org/10.1039/b712066d

5-Amino-5-deoxyguanosine-5-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5-H2N-RNA that can be conjugated to activated esters.

A europium complex that selectively stains nucleoli of cells (2006)
Journal Article
Yu, J., Parker, D., Pal, R., Poole, R., & Cann, M. (2006). A europium complex that selectively stains nucleoli of cells. Journal of the American Chemical Society, 128(7), 2294-2299. https://doi.org/10.1021/ja056303g

A europium complex selectively staining the nucleolus of NIH 3T3, HeLa, and HDF cells is reported. This complex possesses not only the advantage of the long lifetime of europium emission (0.3 ms), but also a chromophore that allows excitation at a re... Read More about A europium complex that selectively stains nucleoli of cells.

Regulation of prokaryotic adenylyl cyclases by CO₂ (2006)
Journal Article
Hammer, A., Hodgson, D., & Cann, M. (2006). Regulation of prokaryotic adenylyl cyclases by CO₂. Biochemical Journal, 396(2), 215-218. https://doi.org/10.1042/bj20060372

The Slr1991 adenylyl cyclase of the model prokaroyte Synechocystis PCC 6803 was stimulated 2-fold at 20 mM total Ci (inorganic carbon) atpH 7.5 through an increase in kcat.Adose response demonstrated an EC50 of 52.7 mMtotal Ci at pH 6.5. Slr1991 aden... Read More about Regulation of prokaryotic adenylyl cyclases by CO₂.

Synthesis and characterisation of highly emissive and kinetically stable lanthanide complexes suitable for usage 'in cellulo' (2005)
Journal Article
Poole, R., Bobba, G., Cann, M., Frias, J., Parker, D., & Peacock, R. (2005). Synthesis and characterisation of highly emissive and kinetically stable lanthanide complexes suitable for usage 'in cellulo'. Organic and Biomolecular Chemistry, 3(6), 1013-1024. https://doi.org/10.1039/b418964g

The synthesis and photophysical characterisation are reported of a series of cationic, neutral and anionic europium and terbium complexes based on structurally related, nonadentate ligands based on the cyclen macrocycle. Each complex incorporates a t... Read More about Synthesis and characterisation of highly emissive and kinetically stable lanthanide complexes suitable for usage 'in cellulo'.

Design, synthesis and evaluation of ratiometric probes for hydrogencarbonate based on europium emission (2004)
Journal Article
Bretonniere, Y., Cann, M., Parker, D., & Slater, R. (2004). Design, synthesis and evaluation of ratiometric probes for hydrogencarbonate based on europium emission. Organic and Biomolecular Chemistry, 2(11), 1624-1632. https://doi.org/10.1039/b400734b

A series of cationic, zwitterionic and anionic macrocyclic europium complexes has been prepared incorporating a N or C- linked acridone chromophore that allows sensitisation of Eu emission following excitation at 390-410nm. Each of these complexes se... Read More about Design, synthesis and evaluation of ratiometric probes for hydrogencarbonate based on europium emission.