All Outputs (7)

The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G.hirsutum) plants (2020)
Journal Article
Wang, Q., Alariqi, M., Wang, F., Li, B., Ding, X., Rui, H., Li, Y., Xu, Z., Qin, L., Sun, L., Li, J., Zou, J., Lindsey, K., Zhang, X., & Jin, S. (2020). The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G.hirsutum) plants. Plant Biotechnology Journal, 18(12), 2436-2443. https://doi.org/10.1111/pbi.13417

The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for genome editing. A new effector with a single nuclease domain, a relatively small size, low frequency off‐target effects and cleavage capability under high temperature has bee... Read More about The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G.hirsutum) plants.

Understanding hormonal crosstalk in Arabidopsis root development via emulation and history matching (2020)
Journal Article
Jackson, S., Vernon, I., Liu, J., & Lindsey, K. (2020). Understanding hormonal crosstalk in Arabidopsis root development via emulation and history matching. Statistical Applications in Genetics and Molecular Biology, 19(2), Article 20180053. https://doi.org/10.1515/sagmb-2018-0053

A major challenge in plant developmental biology is to understand how plant growth is coordinated by interacting hormones and genes. To meet this challenge, it is important to not only use experimental data, but also formulate a mathematical model. F... Read More about Understanding hormonal crosstalk in Arabidopsis root development via emulation and history matching.

Will rising atmospheric CO2 concentration inhibit nitrate assimilation in shoots but enhance it in roots of C3 plants? (2020)
Journal Article
Andrews, M., Condron, L., Kemp, P., Topping, J., Lindsey, K., Hodge, S., & Raven, J. (2020). Will rising atmospheric CO2 concentration inhibit nitrate assimilation in shoots but enhance it in roots of C3 plants?. Physiologia Plantarum, 170(1), 40-45. https://doi.org/10.1111/ppl.13096

Bloom et al. (2019) proposed that rising atmospheric CO2 concentrations “inhibit malate production in chloroplasts and thus impede assimilation of nitrate into protein of C3 plants, a phenomenon that will strongly influence primary productivity and f... Read More about Will rising atmospheric CO2 concentration inhibit nitrate assimilation in shoots but enhance it in roots of C3 plants?.

The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton (2020)
Journal Article
Li, B., Liang, S., Alariqi, M., Wang, F., Wang, G., Wang, Q., Xu, Z., Yu, L., Zafar, M., Sun, L., Si, H., Yuan, D., Guo, W., Wang, Y., Lindsey, K., Zhang, X., & Jin, S. (2020). The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton. Plant Biotechnology Journal, 19(2), 221-223. https://doi.org/10.1111/pbi.13470

Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive and grow well under temperatures up to 45°C. CRISPR/LbCpf1 (LbCas12a) is a temperature‐sensitive system for plant genome editing (Malzahn et al.... Read More about The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free cotton.

Combined GWAS and eQTL analysis uncovers a genetic regulatory network orchestrating the initiation of secondary cell wall development in cotton (2020)
Journal Article
Li, Z., Wang, P., You, C., Yu, J., Zhang, X., Yan, F., Ye, Z., Shen, C., Li, B., Guo, K., Liu, N., Thyssen, G., Fang, D., Lindsey, K., Wang, M., & Tu, L. (2020). Combined GWAS and eQTL analysis uncovers a genetic regulatory network orchestrating the initiation of secondary cell wall development in cotton. New Phytologist, 226(6), 1738-1752. https://doi.org/10.1111/nph.16468

The cotton fiber serves as a valuable experimental system to study cell wall synthesis in plants, but our understanding of the genetic regulation of this process during fiber development remains limited. We performed a genome‐wide association study (... Read More about Combined GWAS and eQTL analysis uncovers a genetic regulatory network orchestrating the initiation of secondary cell wall development in cotton.

CRISPR/Cas systems in genome editing: methodologies and tools for sgRNA design, off-target evaluation and strategies to mitigate off-target effects (2020)
Journal Article
Manghwar, H., Li, B., Ding, X., Hussain, A., Lindsey, K., Zhang, X., & Jin, S. (2020). CRISPR/Cas systems in genome editing: methodologies and tools for sgRNA design, off-target evaluation and strategies to mitigate off-target effects. Advanced Science, 7(6), Article 1902312. https://doi.org/10.1002/advs.201902312

Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas (CRISPR‐associated) sys... Read More about CRISPR/Cas systems in genome editing: methodologies and tools for sgRNA design, off-target evaluation and strategies to mitigate off-target effects.

Vesicle transport in plants: A revised phylogeny of SNARE proteins (2020)
Journal Article
Gu, X., Brennan, A., Wei, W., Guo, G., & Lindsey, K. (2020). Vesicle transport in plants: A revised phylogeny of SNARE proteins. Evolutionary Bioinformatics, 16, 1-11. https://doi.org/10.1177/1176934320956575

Communication systems within and between plant cells involve the transfer of ions and molecules between compartments, and are essential for development and responses to biotic and abiotic stresses. This in turn requires the regulated movement and fus... Read More about Vesicle transport in plants: A revised phylogeny of SNARE proteins.