All Outputs (11)

Designed, Programmable Protein Cages Utilizing Diverse Metal Coordination Geometries Show Reversible, pH‐Dependent Assembly (2024)
Journal Article
Osiński, N., Majsterkiewicz, K., Pakosz-Stępień, Z., Azuma, Y., Biela, A. P., Gaweł, S., & Heddle, J. G. (online). Designed, Programmable Protein Cages Utilizing Diverse Metal Coordination Geometries Show Reversible, pH‐Dependent Assembly. Macromolecular Rapid Communications, Article 2400712. https://doi.org/10.1002/marc.202400712

The rational design and production of a novel series of engineered protein cages are presented, which have emerged as versatile and adaptable platforms with significant applications in biomedicine. These protein cages are assembled from multiple prot... Read More about Designed, Programmable Protein Cages Utilizing Diverse Metal Coordination Geometries Show Reversible, pH‐Dependent Assembly.

Structural basis of chiral wrap and T-segment capture by Escherichia coli DNA gyrase (2024)
Journal Article
Michalczyk, E., Pakosz-Stępień, Z., Liston, J. D., Gittins, O., Pabis, M., Heddle, J. G., & Ghilarov, D. (2024). Structural basis of chiral wrap and T-segment capture by Escherichia coli DNA gyrase. Proceedings of the National Academy of Sciences, 121(49), Article e2407398121. https://doi.org/10.1073/pnas.2407398121

Type II topoisomerase DNA gyrase transduces the energy of ATP hydrolysis into the negative supercoiling of DNA. The postulated catalytic mechanism involves stabilization of a chiral DNA loop followed by the passage of the T-segment through the tempor... Read More about Structural basis of chiral wrap and T-segment capture by Escherichia coli DNA gyrase.

A DNA Origami Bubble Blower for Liposome Production (2024)
Journal Article
Wilkens, G. D., Stępień, P., Sakai, Y., Islam, M. S., & Heddle, J. G. (2024). A DNA Origami Bubble Blower for Liposome Production. ACS Omega, 9(43), 43609–43615. https://doi.org/10.1021/acsomega.4c05297

While liposomes are commonly produced on the industrial scale with diameters in the hundreds of nanometers to micrometer size range, the variation from the mean diameter can be significant. In nature, functional liposome-like systems can be as small... Read More about A DNA Origami Bubble Blower for Liposome Production.

Reengineering of an Artificial Protein Cage for Efficient Packaging of Active Enzymes (2024)
Journal Article
Azuma, Y., Gaweł, S., Pasternak, M., Woźnicka, O., Pyza, E., & Heddle, J. G. (online). Reengineering of an Artificial Protein Cage for Efficient Packaging of Active Enzymes. Small, Article 2312286. https://doi.org/10.1002/smll.202312286

Protein cages that readily encapsulate active enzymes of interest present useful nanotools for delivery and catalysis, wherein those with programmable disassembly characteristics serve as particularly attractive platforms. Here, a general guest packa... Read More about Reengineering of an Artificial Protein Cage for Efficient Packaging of Active Enzymes.

Structure ofEscherichia coliDNA gyrase with chirally wrapped DNA supports ratchet-and-pawl mechanism for an ATP-powered supercoiling motor (2024)
Preprint / Working Paper
Michalczyk, E., Pabiś, M., Heddle, J., & Ghilarov, D. (in press). Structure ofEscherichia coliDNA gyrase with chirally wrapped DNA supports ratchet-and-pawl mechanism for an ATP-powered supercoiling motor

Gyrase is essential for replication and transcription in bacteria, and as such is an important target for antibiotics including fluoroquinolones. Gyrase is a molecular machine that channels the energy of ATP hydrolysis into negative supercoiling of D... Read More about Structure ofEscherichia coliDNA gyrase with chirally wrapped DNA supports ratchet-and-pawl mechanism for an ATP-powered supercoiling motor.

Virus-like particles derived from bacteriophage MS2 as antigen scaffolds and RNA protective shells (2024)
Journal Article
Naskalska, A., & Heddle, J. G. (2024). Virus-like particles derived from bacteriophage MS2 as antigen scaffolds and RNA protective shells. Nanomedicine, 19(12), 1103-1115. https://doi.org/10.2217/nnm-2023-0362

The versatile potential of bacteriophage MS2-derived virus-like particles (VLPs) in medical biotechnology has been extensively studied during the last 30 years. Since the first reports showing that MS2 VLPs can be produced at high yield and relativel... Read More about Virus-like particles derived from bacteriophage MS2 as antigen scaffolds and RNA protective shells.

Running rings around protein cages: a case study of artificial TRAP cages (2024)
Book Chapter
Gaweł, S., Naskalska, A., Osiński, N., & Gardiner Heddle, J. (2024). Running rings around protein cages: a case study of artificial TRAP cages. In M. Ryadnov, & K. Matsuura (Eds.), Amino Acids, Peptides and Proteins: Volume 45 (45-63). Royal Society of Chemistry. https://doi.org/10.1039/bk9781839169328-00045

Proteins that constitute the capsids of viruses are well known for their ability to assemble into cage-like containers. Such containers can carry out useful tasks such as the delivery of cargoes to cells. Here we briefly review our work on naturally-... Read More about Running rings around protein cages: a case study of artificial TRAP cages.

CRAFTing Delivery of Membrane Proteins into Protocells using Nanodiscs (2023)
Journal Article
Stępień, P., Świątek, S., Robles, M. Y. Y., Markiewicz-Mizera, J., Balakrishnan, D., Inaba-Inoue, S., …Heddle, J. G. (2023). CRAFTing Delivery of Membrane Proteins into Protocells using Nanodiscs. ACS Applied Materials and Interfaces, https://doi.org/10.1021/acsami.3c11894

For the successfulgenerativeengineeringof functionalartificialcells,a convenientandcontrollablemeansof deliveringmembraneproteinsinto membranelipidbilayersis necessary.Herewereporta deliverysystemthatachievesthis by employingmembraneprotein-carryingn... Read More about CRAFTing Delivery of Membrane Proteins into Protocells using Nanodiscs.

Production of Metallic Alloy Nanowires and Particles Templated Using Tomato Mosaic Virus (ToMV) (2023)
Journal Article
Shah, S. N., Heddle, J. G., Evans, D. J., & Lomonossoff, G. P. (2023). Production of Metallic Alloy Nanowires and Particles Templated Using Tomato Mosaic Virus (ToMV). Nanomaterials, 13(19), Article 2705. https://doi.org/10.3390/nano13192705

We demonstrate a simple, low-energy method whereby tomato mosaic virus (ToMV) particles can be used to template the production of nanowires and particles consisting of alloys of gold (Au), platinum (Pt) and palladium (Pd) in various combinations. Sel... Read More about Production of Metallic Alloy Nanowires and Particles Templated Using Tomato Mosaic Virus (ToMV).

Inhibitory Compounds Targeting Plasmodium falciparum Gyrase B (2021)
Journal Article
Pakosz, Z., Lin, T., Michalczyk, E., Nagano, S., & Heddle, J. G. (2021). Inhibitory Compounds Targeting Plasmodium falciparum Gyrase B. Antimicrobial Agents and Chemotherapy, 65(10), https://doi.org/10.1128/aac.00267-21

Malaria persists as a major health problem due to the spread of drug resistance and the lack of effective vaccines. DNA gyrase is a well-validated and extremely effective therapeutic target in bacteria, and it is also known to be present in the apico... Read More about Inhibitory Compounds Targeting Plasmodium falciparum Gyrase B.

Enzyme encapsulation by protein cages (2020)
Journal Article
Chakraborti, S., Lin, T., Glatt, S., & Heddle, J. G. (2020). Enzyme encapsulation by protein cages. RSC Advances, 10(22), 13293-13301. https://doi.org/10.1039/c9ra10983h

Protein cages are hollow protein shells with a nanometric cavity that can be filled with useful materials. The encapsulating nature of the cages means that they are particularly attractive for loading with biological macromolecules, affording the gue... Read More about Enzyme encapsulation by protein cages.