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NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity (2023)
Journal Article
Hawkins, T. J., Kopischke, M., Duckney, P. J., Rybak, K., Mentlak, D. A., Kroon, J. T. M., …Robatzek, S. (2023). NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity. Nature Communications, 14(1), Article 5848. https://doi.org/10.1038/s41467-023-41337-z

Members of the NETWORKED (NET) family are involved in actin-membrane interactions. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal cl... Read More about NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity.

STING nuclear partners contribute to innate immune signaling responses (2021)
Journal Article
Dixon, C. R., Malik, P., de las Heras, J. I., Saiz-Ros, N., de Lima Alves, F., Tingey, M., …Schirmer, E. C. (2021). STING nuclear partners contribute to innate immune signaling responses. iScience, 24(9), https://doi.org/10.1016/j.isci.2021.103055

STimulator of INterferon Genes (STING) is an adaptor for cytoplasmic DNA sensing by cGAMP/cGAS that helps trigger innate immune responses (IIRs). Although STING is mostly localized in the ER, we find a separate inner nuclear membrane pool of STING th... Read More about STING nuclear partners contribute to innate immune signaling responses.

BBLN-1 is essential for intermediate filament organization and apical membrane morphology (2021)
Journal Article
Remmelzwaal, S., Geisler, F., Stucchi, R., van der Horst, S., Pasolli, M., Kroll, J. R., …Boxem, M. (2021). BBLN-1 is essential for intermediate filament organization and apical membrane morphology. Current Biology, 31(11), 2334-2346. https://doi.org/10.1016/j.cub.2021.03.069

Epithelial tubes are essential components of metazoan organ systems that control the flow of fluids and the exchange of materials between body compartments and the outside environment. The size and shape of the central lumen confer important characte... Read More about BBLN-1 is essential for intermediate filament organization and apical membrane morphology.

Intestinal intermediate filament polypeptides in C. elegans: Common and isotype-specific contributions to intestinal ultrastructure and function (2020)
Journal Article
Geisler, F., Coch, R. A., Richardson, C., Goldberg, M., Bevilacqua, C., Prevede, R., & Leube, R. E. (2020). Intestinal intermediate filament polypeptides in C. elegans: Common and isotype-specific contributions to intestinal ultrastructure and function. Scientific Reports, 10, Article 3142. https://doi.org/10.1038/s41598-020-59791-w

The abundance and diversity of intermediate filaments (IFs) in the C. elegans intestine indicate important contributions to intestinal function and organismal wellbeing. Fluorescent IF reporters localize below the actin-rich brush border and are high... Read More about Intestinal intermediate filament polypeptides in C. elegans: Common and isotype-specific contributions to intestinal ultrastructure and function.

Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery (2019)
Journal Article
Wang, P., Pleskot, R., Zang, J., Winkler, J., Wang, J., Yperman, K., …Hussey, P. J. (2019). Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery. Nature Communications, 10(1), Article 5132. https://doi.org/10.1038/s41467-019-12782-6

The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these prote... Read More about Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery.

Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication (2019)
Journal Article
Saiz-Ros, N., Czapiewski, R., Epifano, I., Stevenson, A., Swanson, S., Dixon, C., …Schirmer, E. (2019). Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication. Cells, 8(2), Article 120. https://doi.org/10.3390/cells8020120

The primary envelopment/de-envelopment of Herpes viruses during nuclear exit is poorly understood. In Herpes simplex virus type-1 (HSV-1), proteins pUL31 and pUL34 are critical, while pUS3 and some others contribute; however, efficient membrane fusio... Read More about Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication.

The intestinal intermediate filament network responds to and protects against microbial insults and toxins (2019)
Journal Article
Geisler, F., Coch, R. A., Richardson, C., Goldberg, M., Denecke, B., Bossinger, O., & Leube, R. E. (2019). The intestinal intermediate filament network responds to and protects against microbial insults and toxins. Development, 146(2), Article dev.169482. https://doi.org/10.1242/dev.169482

The enrichment of intermediate filaments in the apical cytoplasm of intestinal cells is evolutionary conserved forming a sheath that is anchored to apical junctions and positioned below the microvillar brush border suggestive of a protective intracel... Read More about The intestinal intermediate filament network responds to and protects against microbial insults and toxins.

Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution (2018)
Journal Article
Reipert, S., Goldammer, H., Richardson, C., Goldberg, M. W., Hawkins, T. J., Hollergschwandtner, E., …Stierhof, Y. (2018). Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution. Journal of Histochemistry & Cytochemistry, 66(12), 903-921. https://doi.org/10.1369/0022155418786698

For ultrafast fixation of biological samples to avoid artifacts, high-pressure freezing (HPF) followed by freeze substitution (FS) is preferred over chemical fixation at room temperature. After HPF, samples are maintained at low temperature during de... Read More about Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution.

A rim-and-spoke hypothesis to explain the biomechanical roles for cytoplasmic intermediate filament networks (2017)
Journal Article
Quinlan, R., Schwartz, N., Windoffer, R., Richardson, C., Hawkins, T., Broussard, J., …Leube, R. (2017). A rim-and-spoke hypothesis to explain the biomechanical roles for cytoplasmic intermediate filament networks. Journal of Cell Science, 130(20), 3437-3445. https://doi.org/10.1242/jcs.202168

Textbook images of keratin intermediate filament (IF) networks in epithelial cells and the functional compromization of the epidermis by keratin mutations promulgate a mechanical role for this important cytoskeletal component. In stratified epithelia... Read More about A rim-and-spoke hypothesis to explain the biomechanical roles for cytoplasmic intermediate filament networks.

Repo-Man/PP1 regulates heterochromatin formation in interphase (2017)
Journal Article
Castro, I., Budzak, J., Di Giacinto, M. L., Ligammari, L., Gokhan, E., Spanos, C., …Vagnarelli, P. (2017). Repo-Man/PP1 regulates heterochromatin formation in interphase. Nature Communications, 8, Article 14048. https://doi.org/10.1038/ncomms14048

Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Rep... Read More about Repo-Man/PP1 regulates heterochromatin formation in interphase.

A novel function for the MAP kinase SMA-5 in intestinal tube stability (2016)
Journal Article
Geisler, F., Gerhardus, H., Carberry, K., Davis, W., Jorgensen, E., Richardson, C., …Leube, R. E. (2016). A novel function for the MAP kinase SMA-5 in intestinal tube stability. Molecular Biology of the Cell, 27(24), 3855-3868. https://doi.org/10.1091/mbc.e16-02-0099

Intermediate filaments are major cytoskeletal components whose assembly into complex networks and isotype-specific functions are still largely unknown. Caenorhabditis elegans provides an excellent model system to study intermediate filament organizat... Read More about A novel function for the MAP kinase SMA-5 in intestinal tube stability.

Arabidopsis NAP1 regulates the formation of autophagosomes (2016)
Journal Article
Wang, P., Richardson, C., Hawes, C., & Hussey, P. (2016). Arabidopsis NAP1 regulates the formation of autophagosomes. Current Biology, 26(15), 2060-2069. https://doi.org/10.1016/j.cub.2016.06.008

The SCAR/WAVE complex is required for ARP2/3-mediated actin nucleation, and these complexes are highly conserved in plants and animals [1 and 2]. Proteins from the SCAR/WAVE complex have been found to be membrane associated in plants [3]. Using fluor... Read More about Arabidopsis NAP1 regulates the formation of autophagosomes.

Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development (2016)
Journal Article
Wang, P., Richardson, C., Hawkins, T. J., Sparkes, I., Hawes, C., & Hussey, P. J. (2016). Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development. New Phytologist, 210(4), 1311-1326. https://doi.org/10.1111/nph.13857

The endoplasmic reticulum (ER) is connected to the plasma membrane (PM) through the plant-specific NETWORKED protein, NET3C, and phylogenetically conserved vesicle-associated membrane protein-associated proteins (VAPs). Ten VAP homologues (VAP27-1 to... Read More about Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development.

The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells (2015)
Journal Article
Liu, H., Schneider, H., Recino, A., Richardson, C., Goldberg, M. W., & Rudd, C. E. (2015). The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells. Molecular Cell, 59(5), 840-849. https://doi.org/10.1016/j.molcel.2015.07.015

While immune cell adaptors regulate proximal T cell signaling, direct regulation of the nuclear pore complex (NPC) has not been reported. NPC has cytoplasmic filaments composed of RanGAP1 and RanBP2 with the potential to interact with cytoplasmic med... Read More about The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells.

The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum (2014)
Journal Article
Wang, P., Hawkins, T. J., Richardson, C., Cummins, I., Deeks, M. J., Sparkes, I., …Hussey, P. J. (2014). The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum. Current Biology, 24(12), 1397-1405. https://doi.org/10.1016/j.cub.2014.05.003

The cortical endoplasmic reticulum (ER) network in plants is a highly dynamic structure, and it contacts the plasma membrane (PM) at ER-PM anchor/contact sites. These sites are known to be essential for communication between the ER and PM for lipid t... Read More about The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum.

Nesprin interchain associations control nuclear size. (2012)
Journal Article
Lu, W., Schneider, M., Neumann, S., Jaeger, V., Taranum, S., Munck, M., …Karakesisoglou, I. (2012). Nesprin interchain associations control nuclear size. Cellular and Molecular Life Sciences, 69(20), 3493-3509. https://doi.org/10.1007/s00018-012-1034-1

Nesprins-1/-2/-3/-4 are nuclear envelope proteins, which connect nuclei to the cytoskeleton. The largest nesprin-1/-2 isoforms (termed giant) tether F-actin through their N-terminal actin binding domain (ABD). Nesprin-3, however, lacks an ABD and ass... Read More about Nesprin interchain associations control nuclear size..

A Superfamily of Actin-Binding Proteins at the Actin-Membrane Nexus of Higher Plants. (2012)
Journal Article
Deeks, M. J., Calcutt, J. R., Ingle, E. K., Hawkins, T. J., Chapman, S., Richardson, A., …Hussey, P. J. (2012). at the Actin-Membrane Nexus of Higher Plants. Current Biology, 22(17), 1595-1600. https://doi.org/10.1016/j.cub.2012.06.041

Complex animals use a wide variety of adaptor proteins to produce specialized sites of interaction between actin and membranes. Plants do not have these protein families, yet actin-membrane interactions within plant cells are critical for the positio... Read More about A Superfamily of Actin-Binding Proteins at the Actin-Membrane Nexus of Higher Plants..

System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics (2011)
Journal Article
Zuleger, N., Kelly, D. A., Richardson, A. C., Kerr, A. R., Goldberg, M. W., Goryachev, A. B., & Schirmer, E. C. (2011). System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics. Journal of Cell Biology, 193(1), 109-123. https://doi.org/10.1083/jcb.201009068

The nuclear envelope contains >100 transmembrane proteins that continuously exchange with the endoplasmic reticulum and move within the nuclear membranes. To better understand the organization and dynamics of this system, we compared the trafficking... Read More about System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics.

Modular nanometer-scale structuring of gel fibres by sequential self-organization (2005)
Journal Article
Applegarth, L., Clark, N., Richardson, A., Parker, A., Radosavljevic-Evans, I., Goeta, A., …Steed, J. (2005). Modular nanometer-scale structuring of gel fibres by sequential self-organization. Chemical Communications, 5423-5425

Ag(I) and Cu(II) complexes of a series of simple bis( urea) ligands form soft metallogels. X-ray crystallographic results suggests that the gels' structure is based on hydrogen bonding to counter anions and thus suggests a route to tunable gel rheolo... Read More about Modular nanometer-scale structuring of gel fibres by sequential self-organization.