Structural and functional characterization of the extended-diKH domain from the antiviral endoribonuclease KHNYN

Youle, Rebecca L.; Lista, María José; Bouton, Clement; Kunzelmann, Simone; Wilson, Harry; Cottee, Matthew A.; Purkiss, Andrew G.; Morris, Elizabeth R.; Neil, Stuart J.D.; Taylor, Ian A.; Swanson, Chad M.

Abstract

Zinc finger antiviral protein (ZAP) binds CpG dinucleotides in viral RNA and targets them for decay. ZAP interacts with several cofactors to form the ZAP antiviral system, including KHNYN, a multidomain endoribonuclease required for ZAP-mediated RNA decay. However, it is unclear how the individual domains in KHNYN contribute to its activity. Here, we demonstrate that the KHNYN amino terminal extended-diKH (ex-diKH) domain is required for antiviral activity and present its crystal structure. The structure belongs to a rare group of KH-containing domains, characterized by a non-canonical arrangement between two type-1 KH modules, with an additional helical bundle. N4BP1 is a KHNYN paralog with an ex-diKH domain that functionally complements the KHNYN ex-diKH domain. Interestingly, the ex-diKH domain structure is present in N4BP1-like proteins in lancelets, which are basal chordates, indicating that it is evolutionarily ancient. While many KH domains demonstrate RNA binding activity, biolayer interferometry and electrophoretic mobility shift assays indicate that the KHNYN ex-diKH domain does not bind RNA. Furthermore, residues required for canonical KH domains to bind RNA are not required for KHNYN antiviral activity. By contrast, an inter-KH domain cleft in KHNYN is a potential protein-protein interaction site and mutations that eliminate arginine salt bridges at the edge of this cleft decrease KHNYN antiviral activity. This suggests that this domain could be a binding site for an unknown KHNYN cofactor.

Citation

Youle, R. L., Lista, M. J., Bouton, C., Kunzelmann, S., Wilson, H., Cottee, M. A., Purkiss, A. G., Morris, E. R., Neil, S. J., Taylor, I. A., & Swanson, C. M. (online). Structural and functional characterization of the extended-diKH domain from the antiviral endoribonuclease KHNYN. Journal of Biological Chemistry, Article 108336. https://doi.org/10.1016/j.jbc.2025.108336

• Journal Article Type: Article
• Acceptance Date: Feb 13, 2025
• Online Publication Date: Feb 18, 2025
• Deposit Date: Mar 17, 2025
• Journal: Journal of Biological Chemistry
• Print ISSN: 0021-9258
• Electronic ISSN: 1083-351X
• Publisher: American Society for Biochemistry and Molecular Biology
• Peer Reviewed: Peer Reviewed
• Article Number: 108336
• DOI: https://doi.org/10.1016/j.jbc.2025.108336
• Public URL: https://durham-repository.worktribe.com/output/3715377