Single‐Benzene‐Based Clickable Fluorophores for In Vitro and In Vivo Bioimaging

Mohanrao, Raja; Pinto, Clyde S; Suchenko, Andrejus; Clarkson, Guy J; Wills, Martin; Roesner, Stefan; Shipman, Michael; Balasubramanian, Mohan K

Abstract

A series of miniaturized, clickable single‐benzene‐based fluorophores derived from tetrafluoroterephthalonitrile is reported. Fluorophores based on a tetrahydroquinoxaline skeleton exhibited improved photophysical properties due to enhanced electron delocalization between donor and acceptor groups compared to those with a dihydro[1,4]thiazine skeleton. These easily synthesized clickable fluorophores were successfully applied in both in vitro and in vivo bioimaging following protein conjugation.

Citation

Mohanrao, R., Pinto, C. S., Suchenko, A., Clarkson, G. J., Wills, M., Roesner, S., Shipman, M., & Balasubramanian, M. K. (2025). Single‐Benzene‐Based Clickable Fluorophores for In Vitro and In Vivo Bioimaging. ChemistrySelect, 10(9), Article e202405738. https://doi.org/10.1002/slct.202405738

• Journal Article Type: Article
• Acceptance Date: Jan 20, 2025
• Online Publication Date: Feb 27, 2025
• Publication Date: 2025-03
• Deposit Date: Feb 28, 2025
• Publicly Available Date: Feb 28, 2025
• Journal: ChemistrySelect
• Electronic ISSN: 2365-6549
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Issue: 9
• Article Number: e202405738
• DOI: https://doi.org/10.1002/slct.202405738
• Keywords: Chromophores, Bioimaging, Single‐benzene fluorophores, Bioconjugates, Fluorescent probes
• Public URL: https://durham-repository.worktribe.com/output/3550994