GAP Activity, but Not Subcellular Targeting, Is Required for Arabidopsis RanGAP Cellular and Developmental Functions

Boruc, Joanna; Griffis, Anna H.N.; Rodrigo-Peiris, Thushani; Zhou, Xiao; Tilford, Bailey; Van Damme, Daniël; Meier, Iris

Abstract

The Ran GTPase activating protein (RanGAP) is important to Ran signaling involved in nucleocytoplasmic transport, spindle organization, and postmitotic nuclear assembly. Unlike vertebrate and yeast RanGAP, plant RanGAP has an N-terminal WPP domain, required for nuclear envelope association and several mitotic locations of Arabidopsis thaliana RanGAP1. A double null mutant of the two Arabidopsis RanGAP homologs is gametophyte lethal. Here, we created a series of mutants with various reductions in RanGAP levels by combining a RanGAP1 null allele with different RanGAP2 alleles. As RanGAP level decreases, the severity of developmental phenotypes increases, but nuclear import is unaffected. To dissect whether the GAP activity and/or the subcellular localization of RanGAP are responsible for the observed phenotypes, this series of rangap mutants were transformed with RanGAP1 variants carrying point mutations abolishing the GAP activity and/or the WPP-dependent subcellular localization. The data show that plant development is differentially affected by RanGAP mutant allele combinations of increasing severity and requires the GAP activity of RanGAP, while the subcellular positioning of RanGAP is dispensable. In addition, our results indicate that nucleocytoplasmic trafficking can tolerate both partial depletion of RanGAP and delocalization of RanGAP from the nuclear envelope.

Citation

Boruc, J., Griffis, A. H., Rodrigo-Peiris, T., Zhou, X., Tilford, B., Van Damme, D., & Meier, I. (2015). GAP Activity, but Not Subcellular Targeting, Is Required for Arabidopsis RanGAP Cellular and Developmental Functions. The Plant Cell, 27(7), 1985-1998. https://doi.org/10.1105/tpc.114.135780

• Journal Article Type: Article
• Online Publication Date: Jun 19, 2015
• Publication Date: 2015-07
• Deposit Date: Aug 12, 2024
• Journal: The Plant Cell
• Print ISSN: 1040-4651
• Electronic ISSN: 1532-298X
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 27
• Issue: 7
• Pages: 1985-1998
• DOI: https://doi.org/10.1105/tpc.114.135780
• Public URL: https://durham-repository.worktribe.com/output/2752550
• Additional Information: Available open access via DOI.