Regulation of Proline Accumulation and Protein Secretion in Sorghum under Combined Osmotic and Heat Stress.

Ngwenya, Samkelisiwe P; Moloi, Sellwane J; Shargie, Nemera G; Brown, Adrian P; Chivasa, Stephen; Ngara, Rudo

Abstract

Plants reprogramme their proteome to alter cellular metabolism for effective stress adaptation. Intracellular proteomic responses have been extensively studied, and the extracellular matrix stands as a key hub where peptide signals are generated/processed to trigger critical adaptive signal transduction cascades inaugurated at the cell surface. Therefore, it is important to study the plant extracellular proteome to understand its role in plant development and stress response. This study examined changes in the soluble extracellular sub-proteome of sorghum cell cultures exposed to a combination of sorbitol-induced osmotic stress and heat at 40 °C. The combined stress significantly reduced metabolic activity and altered protein secretion. While cells treated with osmotic stress alone had elevated proline content, the osmoprotectant in the combined treatment remained unchanged, confirming that sorghum cells exposed to combined stress utilise adaptive processes distinct from those invoked by the single stresses applied separately. Reactive oxygen species (ROS)-metabolising proteins and proteases dominated differentially expressed proteins identified in cells subjected to combined stress. ROS-generating peroxidases were suppressed, while ROS-degrading proteins were upregulated for protection from oxidative damage. Overall, our study provides protein candidates that could be used to develop crops better suited for an increasingly hot and dry climate.

Citation

Ngwenya, S. P., Moloi, S. J., Shargie, N. G., Brown, A. P., Chivasa, S., & Ngara, R. (2024). Regulation of Proline Accumulation and Protein Secretion in Sorghum under Combined Osmotic and Heat Stress. Plants, 13(13), Article 1874. https://doi.org/10.3390/plants13131874

• Journal Article Type: Article
• Acceptance Date: Jun 26, 2024
• Online Publication Date: Jul 6, 2024
• Publication Date: Jul 1, 2024
• Deposit Date: Aug 7, 2024
• Publicly Available Date: Aug 7, 2024
• Journal: Plants
• Electronic ISSN: 2223-7747
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 13
• Article Number: 1874
• DOI: https://doi.org/10.3390/plants13131874
• Keywords: iTRAQ, cell suspension cultures, secretome, combined stress, Sorghum bicolor, secreted proteins, extracellular matrix, proteomics
• Public URL: https://durham-repository.worktribe.com/output/2617778