Skip to main content

Research Repository

Advanced Search

Investigation of caliciviruses and astroviruses in Gabonese rodents: A possible influence of national and international trade on the spread of enteric viruses.

Mbou-Boutambe, Clark; Mombo, Illich Manfred; Rougeron, Virginie; Degrugillier, Fanny; Gauthier, Philippe; Makanga, Boris; Ngoubangoye, Barthélemy; Leroy, Eric M; Prugnolle, Franck; Boundenga, Larson

Investigation of caliciviruses and astroviruses in Gabonese rodents: A possible influence of national and international trade on the spread of enteric viruses. Thumbnail


Authors

Clark Mbou-Boutambe

Illich Manfred Mombo

Virginie Rougeron

Fanny Degrugillier

Philippe Gauthier

Boris Makanga

Barthélemy Ngoubangoye

Eric M Leroy

Franck Prugnolle

Larson Boundenga



Abstract

Caliciviruses (Caliciviridae) and astroviruses (Astroviridae) are among the leading cause of non-bacterial foodborne disease and gastroenteritis in human. These non-enveloped RNA viruses infect a wide range of vertebrate species including rodents. Rodents are among the most important hosts of infectious diseases globally and are responsible for over 80 zoonotic pathogens that affect humans. Therefore, screening pathogens in rodents will be is necessary to prevent cross-species transmission to prevent zoonotic outbreaks. In the present study, we screened caliciviruses and astroviruses in order to describe their diversity and whether they harbor strains that can infect humans. RNA was then extracted from intestine samples of 245 rodents and retrotranscribed in cDNA to screen caliciviruses and astroviruses by PCRs. All the samples tested negative for caliciviruses and while astroviruses were detected in 18 (7.3%) samples of Rattus rattus species. Phylogenetic analyses based on the RdRp gene showed that all the sequences belonged to Mamastrovirus genus in which they were genetically related to R. rattus related AstVs previously detected in Gabon or in Rattus spp. AstV from Kenya and Asia. These findings suggested that transportation such as land and railway, as well national and international trade, are likely to facilitate spread of AstVs by the dissemination of rodents.

Citation

Mbou-Boutambe, C., Mombo, I. M., Rougeron, V., Degrugillier, F., Gauthier, P., Makanga, B., …Boundenga, L. (2024). Investigation of caliciviruses and astroviruses in Gabonese rodents: A possible influence of national and international trade on the spread of enteric viruses. Infection, Genetics and Evolution, 122, Article 105607. https://doi.org/10.1016/j.meegid.2024.105607

Journal Article Type Article
Acceptance Date May 21, 2024
Online Publication Date May 26, 2024
Publication Date May 26, 2024
Deposit Date Jun 25, 2024
Publicly Available Date Jun 25, 2024
Journal Infection, Genetics and Evolution
Print ISSN 1567-1348
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 122
Article Number 105607
DOI https://doi.org/10.1016/j.meegid.2024.105607
Keywords Calicivirus, Gabon, Zoonoses, Rodent, Rural areas, Astrovirus
Public URL https://durham-repository.worktribe.com/output/2484081

Files





You might also like



Downloadable Citations