Investigation of caliciviruses and astroviruses in Gabonese rodents: A possible influence of national and international trade on the spread of enteric viruses.

Mbou-Boutambe, Clark; Mombo, Illich Manfred; Rougeron, Virginie; Degrugillier, Fanny; Gauthier, Philippe; Makanga, Boris; Ngoubangoye, Barthélemy; Leroy, Eric M; Prugnolle, Franck; Boundenga, Larson

Abstract

Caliciviruses (Caliciviridae) and astroviruses (Astroviridae) are among the leading cause of non-bacterial foodborne disease and gastroenteritis in human. These non-enveloped RNA viruses infect a wide range of vertebrate species including rodents. Rodents are among the most important hosts of infectious diseases globally and are responsible for over 80 zoonotic pathogens that affect humans. Therefore, screening pathogens in rodents will be is necessary to prevent cross-species transmission to prevent zoonotic outbreaks. In the present study, we screened caliciviruses and astroviruses in order to describe their diversity and whether they harbor strains that can infect humans. RNA was then extracted from intestine samples of 245 rodents and retrotranscribed in cDNA to screen caliciviruses and astroviruses by PCRs. All the samples tested negative for caliciviruses and while astroviruses were detected in 18 (7.3%) samples of Rattus rattus species. Phylogenetic analyses based on the RdRp gene showed that all the sequences belonged to Mamastrovirus genus in which they were genetically related to R. rattus related AstVs previously detected in Gabon or in Rattus spp. AstV from Kenya and Asia. These findings suggested that transportation such as land and railway, as well national and international trade, are likely to facilitate spread of AstVs by the dissemination of rodents.

Citation

Mbou-Boutambe, C., Mombo, I. M., Rougeron, V., Degrugillier, F., Gauthier, P., Makanga, B., …Boundenga, L. (2024). Investigation of caliciviruses and astroviruses in Gabonese rodents: A possible influence of national and international trade on the spread of enteric viruses. Infection, Genetics and Evolution, 122, Article 105607. https://doi.org/10.1016/j.meegid.2024.105607

• Journal Article Type: Article
• Acceptance Date: May 21, 2024
• Online Publication Date: May 26, 2024
• Publication Date: May 26, 2024
• Deposit Date: Jun 25, 2024
• Publicly Available Date: Jun 25, 2024
• Journal: Infection, Genetics and Evolution
• Print ISSN: 1567-1348
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 122
• Article Number: 105607
• DOI: https://doi.org/10.1016/j.meegid.2024.105607
• Keywords: Calicivirus, Gabon, Zoonoses, Rodent, Rural areas, Astrovirus
• Public URL: https://durham-repository.worktribe.com/output/2484081