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The hypoxia response pathway in the Antarctic fish Notothenia coriiceps is functional despite a poly Q/E insertion mutation in HIF-1α.

O'Brien, K M; Rix, A S; Jasmin, A; Lavelle, E

Authors

K M O'Brien

Dr Anna Rix anna.s.rix@durham.ac.uk
PDRA in Antarctic Molecular Ecology

A Jasmin

E Lavelle



Abstract

Antarctic notothenioid fishes, inhabiting the oxygen-rich Southern Ocean, possess a polyglutamine and glutamic acid (poly Q/E) insertion mutation in the master transcriptional regulator of oxygen homeostasis, hypoxia- inducible factor-1α (HIF-1α). To determine if this mutation impairs the ability of HIF-1 to regulate gene expression in response to hypoxia, we exposed Notothenia coriiceps, with a poly Q/E insertion mutation in HIF-1α that is 9 amino acids long, to hypoxia (2.3 mg L O ) or normoxia (10 mg L O ) for 12 h. Heart ventricles, brain, liver, and gill tissue were harvested and changes in gene expression quantified using RNA sequencing. Levels of glycogen and lactate were also quantified to determine if anaerobic metabolism increases in response to hypoxia. Exposure to hypoxia resulted in 818 unique differentially expressed genes (DEGs) in liver tissue of N. coriiceps. Many hypoxic genes were induced, including ones involved in the MAP kinase and FoxO pathways, glycolytic metabolism, and vascular remodeling. In contrast, there were fewer than 104 unique DEGs in each of the other tissues sampled. Lactate levels significantly increased in liver in response to hypoxia, indicating that anaerobic metabolism increases in response to hypoxia in this tissue. Overall, our results indicate that the hypoxia response pathway is functional in N. coriiceps despite a poly Q/E mutation in HIF-1α, and confirm that Antarctic fishes are capable of altering gene expression in response to hypoxia. [Abstract copyright: Copyright © 2024 Elsevier Inc. All rights reserved.]

Journal Article Type Article
Acceptance Date Feb 12, 2024
Online Publication Date Feb 18, 2024
Publication Date 2024-06
Deposit Date May 22, 2024
Publicly Available Date Feb 19, 2025
Journal Comparative biochemistry and physiology - Part D: Genomics and proteomics
Print ISSN 1744-117X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 50
Article Number 101218
DOI https://doi.org/10.1016/j.cbd.2024.101218
Keywords Antarctic fish, Hypoxia, RNA-seq
Public URL https://durham-repository.worktribe.com/output/2330510

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This file is under embargo until Feb 19, 2025 due to copyright restrictions.




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