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Knockout of NMDARs in CA1 and dentate gyrus fails to impair temporal control of conditioned behaviour in mice

Strickland, Jasmin; Austen, Joseph; Sprengel, Rolf; Sanderson, David

Knockout of NMDARs in CA1 and dentate gyrus fails to impair temporal control of conditioned behaviour in mice Thumbnail


Authors

Rolf Sprengel



Contributors

Rolf Sprengel
Researcher

Abstract

The hippocampus has been implicated in temporal learning. Plasticity within the hippocampus requires NMDA receptor-dependent glutamatergic neurotransmission. We tested the prediction that hippocampal NMDA receptors are required for learning about time by testing mice that lack postembryonal NMDARs in the CA1 and dentate gyrus (DG) hippocampal subfields on three different appetitive temporal learning procedures. The conditional knockout mice (Grin1ΔDCA1) showed normal sensitivity to cue duration, responding at a higher level to a short duration cue than compared to a long duration cue. Knockout mice also showed normal precision and accuracy of response timing in the peak procedure in which reinforcement occurred after 10 s delay within a 30 s cue presentation. Mice were tested on the matching of response rates to reinforcement rates on instrumental conditioning with two levers reinforced on a concurrent variable interval schedule. Pressing on one lever was reinforced at a higher rate than the other lever. Grin1ΔDGCA1 mice showed normal sensitivity to the relative reinforcement rates of the levers. In contrast to the lack of effect of hippocampal NMDAR deletion on measures of temporal sensitivity, Grin1ΔDGCA1 mice showed increased baseline measures of magazine activity and lever pressing. Furthermore, reversal learning was enhanced when the reward contingencies were switched in the lever pressing task, but this was true only for mice trained with a large difference between relative reinforcement rates between the levers. The results failed to demonstrate a role for NMDARs in excitatory CA1 and DG neurons in learning about temporal information.

Citation

Strickland, J., Austen, J., Sprengel, R., & Sanderson, D. (2024). Knockout of NMDARs in CA1 and dentate gyrus fails to impair temporal control of conditioned behaviour in mice. Hippocampus, 34(3), 126-140. https://doi.org/10.1002/hipo.23593

Journal Article Type Article
Acceptance Date Nov 25, 2023
Online Publication Date Dec 22, 2023
Publication Date 2024-03
Deposit Date Nov 27, 2023
Publicly Available Date Jan 3, 2024
Journal Hippocampus
Print ISSN 1050-9631
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 34
Issue 3
Pages 126-140
DOI https://doi.org/10.1002/hipo.23593
Public URL https://durham-repository.worktribe.com/output/1961282

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