James Meadows james.w.meadows@durham.ac.uk
PGR Student Doctor of Philosophy
The Effect of Pulling and Twisting Forces on Chameleon Sequence Peptides.
Meadows, James; Röder, Konstantin
Authors
Konstantin Röder
Abstract
Chameleon sequences are amino acid sequences found in several distinct configurations in experiment. They challenge our understanding of the link between sequence and structure, and provide insight into structural competition in proteins. Here, we study the energy landscapes for three such sequences, and interrogate how pulling and twisting forces impact the available structural ensembles. Chameleon sequences do not necessarily exhibit multiple structural ensembles on a multifunnel energy landscape when we consider them in isolation. The application of even small forces leads to drastic changes in the energy landscapes. For pulling forces, we observe transitions from helical to extended structures in a very small span of forces. For twisting forces, the picture is much more complex, and highly dependent on the magnitude and handedness of the applied force as well as the reference angle for the twist. Depending on these parameters, more complex and more simplistic energy landscapes are observed alongside more and less diverse structural ensembles. The impact of even small forces is significant, confirming their likely role in folding events. In addition, small forces exerted by the remaining scaffold of a protein may be sufficient to lead to the adoption of a specific structural ensemble by a chameleon sequence. [Abstract copyright.
Citation
Meadows, J., & Röder, K. (2023). The Effect of Pulling and Twisting Forces on Chameleon Sequence Peptides. ChemPhysChem, 24(24), Article e202300351. https://doi.org/10.1002/cphc.202300351
| Journal Article Type | Article |
|---|---|
| Acceptance Date | Oct 6, 2023 |
| Online Publication Date | Oct 11, 2023 |
| Publication Date | Dec 14, 2023 |
| Deposit Date | Dec 19, 2023 |
| Publicly Available Date | Dec 19, 2023 |
| Journal | Chemphyschem : a European journal of chemical physics and physical chemistry |
| Print ISSN | 1439-4235 |
| Electronic ISSN | 1439-7641 |
| Publisher | Wiley |
| Peer Reviewed | Peer Reviewed |
| Volume | 24 |
| Issue | 24 |
| Article Number | e202300351 |
| DOI | https://doi.org/10.1002/cphc.202300351 |
| Keywords | Energy landscapes, Structural ensembles, Chameleon sequences, Mechanical forces |
| Public URL | https://durham-repository.worktribe.com/output/1819945 |
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