Proteomic study of the soluble proteins from the unicellular cyanobacterium Synechocystis sp PCC6803 using automated matrix-assisted laser desorption/ionization-time of flight peptide mass fingerprinting

Simon, WJ; Hall, JJ; Suzuki, I; Murata, N; Slabas, AR

Abstract

The unicellular cyanobacteria Synechocystis sp. (PCC6803) has become a model organism for a range of biochemical and molecular biology studies aimed at investigating environmental stress responses. In this study the soluble proteins of Synechocystis were analysed using narrow pH range (pH 4.5-5.5) zoom gels, automated matrix-assisted laser desorption/ionization mass spectrometry acquisition, spectral processing and database searching. The work sets the foundation for investigations of proteomic changes following stress treatment. One hundred and ninety-two protein spots were analysed and 105 proteins identified, of these 37 were novel proteins not previously seen on two-dimensional gels. Proteins involved in amino acid biosynthesis, energy metabolism and protein modification were identified using this fully automated procedure demonstrating that automated acquisition and processing will be a useful tool for proteomic analyses on this organism.

Citation

Simon, W., Hall, J., Suzuki, I., Murata, N., & Slabas, A. (2002). Proteomic study of the soluble proteins from the unicellular cyanobacterium Synechocystis sp PCC6803 using automated matrix-assisted laser desorption/ionization-time of flight peptide mass fingerprinting. Proteomics, 2(12), 1735-1742. https://doi.org/10.1002/1615-9861%28200212%292%3A12%3C1735%3A%3Aaid-prot1735%3E3.0.co%3B2-k

• Journal Article Type: Article
• Publication Date: Dec 1, 2002
• Deposit Date: May 18, 2007
• Journal: Proteomics
• Print ISSN: 1615-9853
• Electronic ISSN: 1615-9861
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 2
• Issue: 12
• Pages: 1735-1742
• DOI: https://doi.org/10.1002/1615-9861%28200212%292%3A12%3C1735%3A%3Aaid-prot1735%3E3.0.co%3B2-k
• Keywords: Automated matrix-assisted laser desorption/ionization-mass spectrometry analysis, Cyanobacteria, Synechocystis.
• Public URL: https://durham-repository.worktribe.com/output/1548500