G Salpingidou
A novel role for the nuclear membrane protein emerin in association of the centrosome to the outer nuclear membrane
Salpingidou, G; Smertenko, A; Hausmanowa-Petrucewicz, I; Hussey, PJ; Hutchison, CJ
Authors
A Smertenko
I Hausmanowa-Petrucewicz
PJ Hussey
CJ Hutchison
Abstract
The type II inner nuclear membrane protein emerin is a component of the LINC complex that connects the nuclear lamina to the actin cytoskeleton. In emerin-null or -deficient human dermal fibroblasts we find that the centrosome is detached from the nucleus. Moreover, following siRNA knockdown of emerin in wild-type fibroblasts, the centrosome also becomes detached from the nucleus. We show that emerin interacts with tubulin, and that nocadozole-treated wild-type cells phenocopy the detached centrosome characteristic of emerin-null/deficient cells. We also find that a significant fraction of emerin is located at the outer nuclear membrane and peripheral ER, where it interacts directly with the centrosome. Our data provide the first evidence in mammalian cells as to the nature of the linkage of the centrosome, and therefore the tubulin cytoskeleton, with the outer nuclear membrane.
Citation
Salpingidou, G., Smertenko, A., Hausmanowa-Petrucewicz, I., Hussey, P., & Hutchison, C. (2007). A novel role for the nuclear membrane protein emerin in association of the centrosome to the outer nuclear membrane. Journal of Cell Biology, 178(6), 897-904. https://doi.org/10.1083/jcb.200702026
Journal Article Type | Article |
---|---|
Publication Date | Sep 1, 2007 |
Deposit Date | Aug 29, 2008 |
Publicly Available Date | Aug 29, 2008 |
Journal | Journal of Cell Biology |
Print ISSN | 0021-9525 |
Electronic ISSN | 1540-8140 |
Publisher | Rockefeller University Press |
Peer Reviewed | Peer Reviewed |
Volume | 178 |
Issue | 6 |
Pages | 897-904 |
DOI | https://doi.org/10.1083/jcb.200702026 |
Public URL | https://durham-repository.worktribe.com/output/1534357 |
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