‘Multicopy Multivalent’ Glycopolymer-Stabilized Gold Nanoparticles as Potential Synthetic Cancer Vaccines

Parry, AL; Clemson, NA; Ellis, J; Bernhard, SSR; Davis, BG; Cameron, NR.

Abstract

Mucin-related carbohydrates are overexpressed on the surface of cancer cells, providing a disease-specific target for cancer immunotherapy. Here, we describe the design and construction of peptide-free multivalent glycosylated nanoscale constructs as potential synthetic cancer vaccines that generate significant titers of antibodies selective for aberrant mucin glycans. A polymerizable version of the Tn-antigen glycan was prepared and converted into well-defined glycopolymers by Reversible Addition–Fragmentation chain Transfer (RAFT) polymerization. The polymers were then conjugated to gold nanoparticles, yielding ‘multicopy-multivalent’ nanoscale glycoconjugates. Immunological studies indicated that these nanomaterials generated strong and long-lasting production of antibodies that are selective to the Tn-antigen glycan and cross-reactive toward mucin proteins displaying Tn. The results demonstrate proof-of-concept of a simple and modular approach toward synthetic anticancer vaccines based on multivalent glycosylated nanomaterials without the need for a typical vaccine protein component.

Citation

Parry, A., Clemson, N., Ellis, J., Bernhard, S., Davis, B., & Cameron, N. (2013). ‘Multicopy Multivalent’ Glycopolymer-Stabilized Gold Nanoparticles as Potential Synthetic Cancer Vaccines. Journal of the American Chemical Society, 135(25), 9362-9365. https://doi.org/10.1021/ja4046857

• Journal Article Type: Article
• Publication Date: Jun 1, 2013
• Deposit Date: Sep 15, 2013
• Publicly Available Date: Mar 19, 2014
• Journal: Journal of the American Chemical Society
• Print ISSN: 0002-7863
• Electronic ISSN: 1520-5126
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 135
• Issue: 25
• Pages: 9362-9365
• DOI: https://doi.org/10.1021/ja4046857
• Public URL: https://durham-repository.worktribe.com/output/1469826