Expression of matrix metalloproteinase-10 in human bladder transitional cell carcinoma

Seargent, J.M; Loadman, P.M; Martin, S.W; Naylor, B; Bibby, M.C; Gill, J.H

Abstract

OBJECTIVES: To analyze matrix metalloproteinase-10 (MMP-10) expression in transitional cell carcinoma (TCC) of the bladder, evaluate the correlations between MMP-10 protein expression and clinicopathologic parameters, and address the viability of MMP-10 as a therapeutic target for TCC. MMP-mediated degradation of the extracellular matrix is an important factor in the pathogenesis of tumorigenesis and metastasis. METHODS: Using immunohistochemistry, the expression of MMP-10 was assessed using both tissue microarrays and whole sections of archival tissue specimens representative of all grades and stages of human bladder TCC (n = 60). MMP-10 expression was also assessed in histologically normal human bladder tissue (n = 10). The immunostaining results for MMP-10 expression were examined for correlations with tumor grade and stage. RESULTS: Unlike most MMPs, MMP-10 was localized primarily in the tumor mass as opposed to the tumor stroma and was detectable in all grades and stages of TCC. Significantly greater levels of MMP-10 protein were observed in superficial (pTa, pT1; n = 38) tumors than in normal bladder tissue (P = 0.01). In contrast to the proposed role of MMPs in tumor invasion, no significant difference was observed between muscle-invasive tumors (pT2 or worse; n = 22) and histologically normal bladder tissue (P = 0.50). MMP-10 expression showed no significant correlation with tumor grade. CONCLUSIONS: The data from our study showed that, unlike most MMPs, MMP-10 was not associated with tumor aggression or invasion. Our results suggest that MMP-10 protein levels are significantly greater in the earlier stages of TCC development.

Citation

Seargent, J., Loadman, P., Martin, S., Naylor, B., Bibby, M., & Gill, J. (2005). Expression of matrix metalloproteinase-10 in human bladder transitional cell carcinoma. Urology, 65(4), 815-820. https://doi.org/10.1016/j.urology.2004.11.016

• Journal Article Type: Article
• Publication Date: Apr 1, 2005
• Deposit Date: Jan 9, 2013
• Publicly Available Date: Feb 12, 2013
• Journal: Urology
• Print ISSN: 0090-4295
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 65
• Issue: 4
• Pages: 815-820
• DOI: https://doi.org/10.1016/j.urology.2004.11.016