Network balance via CRY signalling controls the Arabidopsis circadian clock over ambient temperatures

Gould, Peter D.; Ugarte, Nicolas; Domija, Mirela; Costa, Maria; Foreman, Julia; MacGregor, Dana; Rose, Ken; Griffiths, Jayne; Millar, Andrew J.; Finkenstädt, Bärbel; Penfield, Steven; Rand, David A.; Halliday, Karen J.; Hall, Anthony J.W.

Abstract

Circadian clocks exhibit ‘temperature compensation’, meaning that they show only small changes in period over a broad temperature range. Several clock genes have been implicated in the temperature‐dependent control of period in Arabidopsis. We show that blue light is essential for this, suggesting that the effects of light and temperature interact or converge upon common targets in the circadian clock. Our data demonstrate that two cryptochrome photoreceptors differentially control circadian period and sustain rhythmicity across the physiological temperature range. In order to test the hypothesis that the targets of light regulation are sufficient to mediate temperature compensation, we constructed a temperature‐compensated clock model by adding passive temperature effects into only the light‐sensitive processes in the model. Remarkably, this model was not only capable of full temperature compensation and consistent with mRNA profiles across a temperature range, but also predicted the temperature‐dependent change in the level of LATE ELONGATED HYPOCOTYL, a key clock protein. Our analysis provides a systems‐level understanding of period control in the plant circadian oscillator.

Citation

Gould, P. D., Ugarte, N., Domija, M., Costa, M., Foreman, J., MacGregor, D., …Hall, A. J. (2013). Network balance via CRY signalling controls the Arabidopsis circadian clock over ambient temperatures. Molecular Systems Biology, 9(1), Article 650. https://doi.org/10.1038/msb.2013.7

• Journal Article Type: Article
• Acceptance Date: Jan 28, 2013
• Online Publication Date: Mar 19, 2013
• Publication Date: Mar 19, 2013
• Deposit Date: Jan 17, 2017
• Publicly Available Date: Feb 2, 2017
• Journal: Molecular Systems Biology
• Publisher: EMBO Press
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 1
• Article Number: 650
• DOI: https://doi.org/10.1038/msb.2013.7
• Public URL: https://durham-repository.worktribe.com/output/1396403