DNA Methylation Profiling using Infinium Methylation Assay

Zeller, C.; Masrour, N.; Patel, N.; Dai, W.; Wilhelm-Benartzi, C.; Brown, R.

Abstract

The Infinium Human Methylation 450 BeadChip technology allows the rapid quantitative DNA methylation analysis of more than 485,000 CpG dinucleotides located across the genome. The method utilizes sodium bisulfite treatment of genomic DNA to convert unmethylated cytosine residues into uracils whereas methylated cytosines remain unchanged. Modified DNA is then whole genome amplified, fragmented and hybridized to locus-specific oligomer probes linked to individual beads on a BeadChip. Hybridization is followed by single-base extension of the oligomer with a labeled nucleotide. The BeadChip is subsequently fluorescently stained and scanned to measure the intensities of the beads corresponding to the unmethylated and methylated CpG sites.

Citation

Zeller, C., Masrour, N., Patel, N., Dai, W., Wilhelm-Benartzi, C., & Brown, R. (2013). DNA Methylation Profiling using Infinium Methylation Assay. Bio-protocol, 3(10), Article e777. https://doi.org/10.21769/bioprotoc.777

• Journal Article Type: Article
• Online Publication Date: May 20, 2013
• Publication Date: May 20, 2013
• Deposit Date: Jun 3, 2016
• Publicly Available Date: Dec 15, 2017
• Journal: Bio-protocol
• Electronic ISSN: 2331-8325
• Publisher: Bio-Protocol
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Issue: 10
• Article Number: e777
• DOI: https://doi.org/10.21769/bioprotoc.777
• Public URL: https://durham-repository.worktribe.com/output/1381468