Shing-Hong Lin
Evidence for the involvement of ASIC3 in sensory mechanotransduction in proprioceptors
Lin, Shing-Hong; Cheng, Yuan-Ren; Banks, Robert W.; Min, Ming-Yuan; Bewick, Guy S.; Chen, Chih-Cheng
Authors
Yuan-Ren Cheng
Robert Banks r.w.banks@durham.ac.uk
Academic Visitor
Ming-Yuan Min
Guy S. Bewick
Chih-Cheng Chen
Abstract
Acid-sensing ion channel 3 (ASIC3) is involved in acid nociception, but its possible role in neurosensory mechanotransduction is disputed. We report here the generation of Asic3-knockout/eGFPf-knockin mice and subsequent characterization of heterogeneous expression of ASIC3 in the dorsal root ganglion (DRG). ASIC3 is expressed in parvalbumin (Pv+) proprioceptor axons innervating muscle spindles. We further generate a floxed allele of Asic3 (Asic3f/f) and probe the role of ASIC3 in mechanotransduction in neurite-bearing Pv+ DRG neurons through localized elastic matrix movements and electrophysiology. Targeted knockout of Asic3 disrupts spindle afferent sensitivity to dynamic stimuli and impairs mechanotransduction in Pv+ DRG neurons because of substrate deformation-induced neurite stretching, but not to direct neurite indentation. In behavioural tasks, global knockout (Asic3−/−) and Pv-Cre::Asic3f/f mice produce similar deficits in grid and balance beam walking tasks. We conclude that, at least in mouse, ASIC3 is a molecular determinant contributing to dynamic mechanosensitivity in proprioceptors.
Citation
Lin, S., Cheng, Y., Banks, R. W., Min, M., Bewick, G. S., & Chen, C. (2016). Evidence for the involvement of ASIC3 in sensory mechanotransduction in proprioceptors. Nature Communications, 7, Article 11460. https://doi.org/10.1038/ncomms11460
Journal Article Type | Article |
---|---|
Acceptance Date | Mar 29, 2016 |
Online Publication Date | May 10, 2016 |
Publication Date | May 10, 2016 |
Deposit Date | Jun 27, 2016 |
Publicly Available Date | Jul 13, 2016 |
Journal | Nature Communications |
Publisher | Nature Research |
Peer Reviewed | Peer Reviewed |
Volume | 7 |
Article Number | 11460 |
DOI | https://doi.org/10.1038/ncomms11460 |
Public URL | https://durham-repository.worktribe.com/output/1380397 |
Files
Published Journal Article
(2.4 Mb)
PDF
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
Copyright Statement
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
You might also like
Synapsin I/II knock out disrupts mechanosensory terminal firing and motor behaviour.
(2010)
Presentation / Conference Contribution
Glutamatergic modulation of vesicle turnover in primary mechanosensory endings
(2009)
Presentation / Conference Contribution
Development of fluorescent and biotinylated agonists for a novel glutamate receptor in mechanosensory terminals.
(2013)
Presentation / Conference Contribution
The role of synaptic-like vesicles in control of mechanosensation in peripheral nerve terminals.
(2010)
Presentation / Conference Contribution
Downloadable Citations
About Durham Research Online (DRO)
Administrator e-mail: dro.admin@durham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search