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Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability

Dubińska-Magiera, Magda; Chmielewska, Magdalena; Kozioł, Katarzyna; Machowska, Magdalena; Hutchison, Christopher J.; Goldberg, Martin W.; Rzepecki, Ryszard

Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability Thumbnail


Authors

Magda Dubińska-Magiera

Magdalena Chmielewska

Katarzyna Kozioł

Magdalena Machowska

Christopher J. Hutchison

Ryszard Rzepecki



Abstract

Xenopus LAP2β protein is the single isoform expressed in XTC cells. The protein localizes on heterochromatin clusters both at the nuclear envelope and inside a cell nucleus. The majority of XLAP2β fraction neither colocalizes with TPX2 protein during interphase nor can be immunoprecipitated with XLAP2β antibody. Knockdown of the XLAP2β protein expression in XTC cells by synthetic siRNA and plasmid encoded siRNA resulted in nuclear abnormalities including changes in shape of nuclei, abnormal chromatin structure, loss of nuclear envelope, mislocalization of integral membrane proteins of INM such as lamin B2, mislocalization of nucleoporins, and cell death. Based on timing of cell death, we suggest mechanism associated with nucleus reassembly or with entry into mitosis. This confirms that Xenopus LAP2 protein is essential for the maintenance of cell nucleus integrity and the process of its reassembly after mitosis.

Citation

Dubińska-Magiera, M., Chmielewska, M., Kozioł, K., Machowska, M., Hutchison, C. J., Goldberg, M. W., & Rzepecki, R. (2015). Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability. Protoplasma, 253(3), 943-956. https://doi.org/10.1007/s00709-015-0861-y

Journal Article Type Article
Acceptance Date Jul 15, 2015
Online Publication Date Jul 25, 2015
Publication Date Jul 25, 2015
Deposit Date Jun 23, 2017
Publicly Available Date Jun 23, 2017
Journal Protoplasma
Print ISSN 0033-183X
Electronic ISSN 1615-6102
Publisher Springer
Peer Reviewed Peer Reviewed
Volume 253
Issue 3
Pages 943-956
DOI https://doi.org/10.1007/s00709-015-0861-y
Public URL https://durham-repository.worktribe.com/output/1356580

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.






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