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Continual trials spontaneous recognition tasks in mice: reducing animal numbers and improving our understanding of the mechanisms underlying memory

Chan, Michele; Eacott, Madeline J.; Sanderson, David J.; Wang, Jianfei; Sun, Mu; Easton, Alexander

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Authors

Michele Chan

Madeline J. Eacott

Jianfei Wang

Mu Sun



Abstract

Spontaneous recognition tasks are widely used as a laboratory measure of memory in animals but give rise to high levels of behavioural noise leading to a lack of reliability. Previous work has shown that a modification of the procedure to allow continual trials testing (in which many trials are run concurrently in a single session) decreases behavioural noise and thus significantly reduces the numbers of rats required to retain statistical power. Here we demonstrate for the first time that this improved method of testing extends to mice, increasing the overall power of the approach. Moreover, our results show that the new continual trials approach provides the additional benefits of heightened sensitivity and thus provides greater insight into the mechanisms at play. Standard (c57) and transgenic Alzheimer model (TASTPM) mice were tested both at 7 and 10 months of age in both object recognition (OR) and object location (OL) spontaneous recognition tasks using the continual trials methodology. Both c57 and TASTPM mice showed age-dependent changes in performance in OR. While c57 mice also showed age-related changes in performance of OL, TASTPM mice were unable to perform OL at either age. Significantly, we demonstrate that differences in OL performance in c57s and TASTPM animals is a result of proactive interference rather than an absolute inability to recognise object-location combinations. We argue that these continual trials approaches provide overall improved reliability and better interpretation of the memory ability of mice, as well as providing a significant reduction in overall animal use.

Citation

Chan, M., Eacott, M. J., Sanderson, D. J., Wang, J., Sun, M., & Easton, A. (2018). Continual trials spontaneous recognition tasks in mice: reducing animal numbers and improving our understanding of the mechanisms underlying memory. Frontiers in Behavioral Neuroscience, 12, Article 214. https://doi.org/10.3389/fnbeh.2018.00214

Journal Article Type Article
Acceptance Date Aug 27, 2018
Online Publication Date Sep 13, 2018
Publication Date Sep 13, 2018
Deposit Date Aug 28, 2018
Publicly Available Date Sep 13, 2018
Journal Frontiers in Behavioral Neuroscience
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 12
Article Number 214
DOI https://doi.org/10.3389/fnbeh.2018.00214

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Copyright: © 2018 Chan, Eacott, Sanderson, Wang, Sun and Easton. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.







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