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Apoptotic blebs from Leishmania major-infected macrophages as a new approach for cutaneous leishmaniasis vaccination

Faridnia, Roghiyeh; Kalani, Hamed; Hezarjaribi, Hajar Ziaei; Denny, Paul W.; Rafie, Alireza; Fakhar, Mahdi; Virgilio, Stela

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Authors

Roghiyeh Faridnia

Hamed Kalani

Hajar Ziaei Hezarjaribi

Alireza Rafie

Mahdi Fakhar

Stela Virgilio



Abstract

We focused on apoptotic blebs from Leishmania major-infected macrophages as a vaccine for cutaneous leishmaniasis. Apoptosis was induced in L. major-infected J774A.1 cells in order to prepare apoptotic blebs. Test groups of BALB/c mice were immunized with these at doses of 1 × 106, 5 × 106 or 1 × 107 blebs. An immunization control group received Leishmania lysate antigens. The results showed that as the number of apoptotic bodies increased, the lymphocyte proliferation index increased, and this was proportional to IFN-γ level in the test groups. Additionally, the difference of IFN-γ, IL-4, IFN-γ/IL-4 ratio, or total IgG (p < 0.0001) in all groups was statistically significant compared to the negative control group. The highest IFN-γ (514.0 ± 40.92 pg/mL) and IFN-γ/IL-4 ratio (2.94 ± 0.22) were observed in the group that received 1 × 107 apoptotic blebs. The highest levels of IL-4 (244.6 ± 38.8 pg/mL) and total IgG (5626 ± 377 μg/mL) were observed in the immunization control group. Reflecting these data, no lesions were observed in any of the groups vaccinated with apoptotic blebs after 12 weeks. In summary, the use of apoptotic blebs from L. major-infected macrophages is protective against the challenge with L. major in this animal model.

Citation

Faridnia, R., Kalani, H., Hezarjaribi, H. Z., Denny, P. W., Rafie, A., Fakhar, M., & Virgilio, S. (2020). Apoptotic blebs from Leishmania major-infected macrophages as a new approach for cutaneous leishmaniasis vaccination. Microbial Pathogenesis, 147, Article 104406. https://doi.org/10.1016/j.micpath.2020.104406

Journal Article Type Article
Acceptance Date Jul 17, 2020
Online Publication Date Jul 30, 2020
Publication Date 2020-10
Deposit Date Jul 30, 2020
Publicly Available Date Jul 30, 2021
Journal Microbial Pathogenesis
Print ISSN 0882-4010
Electronic ISSN 1096-1208
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 147
Article Number 104406
DOI https://doi.org/10.1016/j.micpath.2020.104406
Public URL https://durham-repository.worktribe.com/output/1295637

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