Isolation and characterisation of bacteriophages with activity against invasive non-typhoidal Salmonella causing blood-stream infection in Malawi

Rodwell, E.V.; Wenner, N.; Pulford, C.V.; Cai, Y.; Bowers-Barnard, A.; Beckett, A.; Rigby, J.; Picton, D.M.; Blower, T.R.; Feasey, N.A.; Hinton, J.C.D.; Perez-Sepulveda, B.M.

Abstract

In recent years, novel lineages of invasive non-typhoidal Salmonella (iNTS) serovars Typhimurium and Enteritidis have been identified in patients with bloodstream infection in sub-Saharan Africa. Here, we isolated and characterised 32 phages capable of infecting S. Typhimurium and S. Enteritidis, from water sources in Malawi and the UK. The phages were classified in three major phylogenetic clusters that were geographically distributed. In terms of host range, Cluster 1 phages were able to infect all bacterial hosts tested, whereas Clusters 2 and 3 had a more restricted profile. Cluster 3 contained two sub-clusters, and 3.b contained the most novel isolates. This study represents the first exploration of the potential for phages to target the lineages of Salmonella that are responsible for bloodstream infections in sub-Saharan Africa.

Citation

Rodwell, E., Wenner, N., Pulford, C., Cai, Y., Bowers-Barnard, A., Beckett, A., …Perez-Sepulveda, B. (2021). Isolation and characterisation of bacteriophages with activity against invasive non-typhoidal Salmonella causing blood-stream infection in Malawi. Viruses, 13(3), Article 478. https://doi.org/10.3390/v13030478

• Journal Article Type: Article
• Acceptance Date: Mar 11, 2021
• Online Publication Date: Mar 15, 2021
• Publication Date: 2021-03
• Deposit Date: Mar 11, 2021
• Publicly Available Date: Mar 18, 2021
• Journal: Viruses
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 3
• Article Number: 478
• DOI: https://doi.org/10.3390/v13030478
• Public URL: https://durham-repository.worktribe.com/output/1279274
• Related Public URLs: https://doi.org/10.1101/2021.02.18.431836