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Defeating the trypanosomatid trio: proteomics of the protozoan parasites causing neglected tropical diseases

Parthasarathy, Anutthaman; Karunakaran, Kalesh

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Authors

Anutthaman Parthasarathy



Abstract

Mass spectrometry-based proteomics enables accurate measurement of the modulations of proteins on a large scale upon perturbation and facilitates the understanding of the functional roles of proteins in biological systems. It is a particularly relevant methodology for studying Leishmania spp., Trypanosoma cruzi and Trypanosoma brucei, as the gene expression in these parasites is primarily regulated by posttranscriptional mechanisms. Large-scale proteomics studies have revealed a plethora of information regarding modulated proteins and their molecular interactions during various life processes of the protozoans, including stress adaptation, life cycle changes and interactions with the host. Important molecular processes within the parasite that regulate the activity and subcellular localisation of its proteins, including several co- and post-translational modifications, are also accurately captured by modern proteomics mass spectrometry techniques. Finally, in combination with synthetic chemistry, proteomic techniques facilitate unbiased profiling of targets and off-targets of pharmacologically active compounds in the parasites. This provides important data sets for their mechanism of action studies, thereby aiding drug development programmes.

Citation

Parthasarathy, A., & Karunakaran, K. (2020). Defeating the trypanosomatid trio: proteomics of the protozoan parasites causing neglected tropical diseases. RSC Medicinal Chemistry, 11(6), 625-645. https://doi.org/10.1039/d0md00122h

Journal Article Type Article
Acceptance Date May 12, 2020
Online Publication Date May 22, 2020
Publication Date Jun 1, 2020
Deposit Date May 24, 2020
Publicly Available Date May 26, 2020
Journal RSC medicinal chemistry.
Electronic ISSN 2632-8682
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 11
Issue 6
Pages 625-645
DOI https://doi.org/10.1039/d0md00122h
Public URL https://durham-repository.worktribe.com/output/1263785

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