Jungwoo Yang
Zmo0994, a novel LEA-like protein from Zymomonas mobilis, increases multi-abiotic stress tolerance in Escherichia coli
Yang, Jungwoo; Kim, Ha Eun; Jung, Young Hoon; Kim, Jungyeon; Kim, Do Hyoung; Walmsley, Adrian R.; Kim, Kyoung Heon
Authors
Ha Eun Kim
Young Hoon Jung
Jungyeon Kim
Do Hyoung Kim
Adrian R. Walmsley
Kyoung Heon Kim
Abstract
Background: Pretreatment processes and subsequent enzymatic hydrolysis are prerequisites to utilize lignocellulosic sugar for fermentation. However, the resulting hydrolysate frequently hinders fermentation processes due to the presence of inhibitors and toxic products (e.g., ethanol). Thus, it is crucial to develop robust microbes conferring multi-stress tolerance. Results: Zmo0994, a functionally uncharacterized protein from Zymomonas mobilis, was identified and characterized for the first time. A major effect of Zmo0994 was a significant enhancement in the tolerance to abiotic stresses such as ethanol, furfural, 5′-hydroxymethylfurfural and high temperature, when expressed in Escherichia coli. Through transcriptome analysis and in vivo experiments, the cellular mechanism of this protein was revealed as due to its ability to trigger genes, involved in aerobic respiration for ATP synthesis. Conclusions: These findings have significant implications that might lead to the development of robust microbes for the highly efficient industrial fermentation processes.
Citation
Yang, J., Kim, H. E., Jung, Y. H., Kim, J., Kim, D. H., Walmsley, A. R., & Kim, K. H. (2020). Zmo0994, a novel LEA-like protein from Zymomonas mobilis, increases multi-abiotic stress tolerance in Escherichia coli. Biotechnology for Biofuels, 13(1), Article 151. https://doi.org/10.1186/s13068-020-01790-0
| Journal Article Type | Article |
|---|---|
| Acceptance Date | Aug 16, 2020 |
| Online Publication Date | Aug 26, 2020 |
| Publication Date | 2020 |
| Deposit Date | Sep 3, 2020 |
| Publicly Available Date | Sep 3, 2020 |
| Journal | Biotechnology for Biofuels |
| Electronic ISSN | 1754-6834 |
| Publisher | BioMed Central |
| Peer Reviewed | Peer Reviewed |
| Volume | 13 |
| Issue | 1 |
| Article Number | 151 |
| DOI | https://doi.org/10.1186/s13068-020-01790-0 |
| Public URL | https://durham-repository.worktribe.com/output/1262890 |
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