Skip to main content

Research Repository

Advanced Search

CPR-C4 is a highly conserved novel protease from the Candidate Phyla Radiation with remote structural homology to human vasohibins

Cornish, Katy A.S.; Lange, Joanna; Aevarsson, Arnthór; Pohl, Ehmke

CPR-C4 is a highly conserved novel protease from the Candidate Phyla Radiation with remote structural homology to human vasohibins Thumbnail


Authors

Katy A.S. Cornish

Joanna Lange

Arnthór Aevarsson



Abstract

The Candidate Phyla Radiation is a recently uncovered and vast expansion of the bacterial domain of life, made up of largely uncharacterized phyla that lack isolated representatives. This unexplored territory of genetic diversity presents an abundance of novel proteins with potential applications in the life-science sectors. Here, we present the structural and functional elucidation of CPR-C4, a hypothetical protein from the genome of a thermophilic Candidate Phyla Radiation organism, identified through metagenomic sequencing. Our analyses revealed that CPR-C4 is a member of a family of highly conserved proteins within the Candidate Phyla Radiation. The function of CPR-C4 as a cysteine protease was predicted through remote structural similarity to the Homo sapiens vasohibins and subsequently confirmed experimentally with fluorescence-based activity assays. Furthermore, detailed structural and sequence alignment analysis enabled identification of a noncanonical cysteine-histidine-leucine(carbonyl) catalytic triad. The unexpected structural and functional similarities between CPR-C4 and the human vasohibins suggest an evolutionary relationship undetectable at the sequence level alone.

Citation

Cornish, K. A., Lange, J., Aevarsson, A., & Pohl, E. (2022). CPR-C4 is a highly conserved novel protease from the Candidate Phyla Radiation with remote structural homology to human vasohibins. Journal of Biological Chemistry, 298(5), Article 101919. https://doi.org/10.1016/j.jbc.2022.101919

Journal Article Type Article
Acceptance Date Apr 4, 2022
Online Publication Date May 10, 2022
Publication Date 2022-05
Deposit Date Jun 21, 2022
Publicly Available Date Jun 21, 2022
Journal Journal of Biological Chemistry
Print ISSN 0021-9258
Electronic ISSN 1083-351X
Publisher American Society for Biochemistry and Molecular Biology
Peer Reviewed Peer Reviewed
Volume 298
Issue 5
Article Number 101919
DOI https://doi.org/10.1016/j.jbc.2022.101919

Files






You might also like



Downloadable Citations