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Overexpression of phospholipid: diacylglycerol acyltransferase in Brassica napus results in changes in lipid metabolism and oil accumulation (2022)
Journal Article
Fenyk, S., Woodfield, H. K., Romsdahl, T. B., Wallington, E. J., Bates, R. E., Fell, D. A., Chapman, K. D., Fawcett, T., & Harwood, J. L. (2022). Overexpression of phospholipid: diacylglycerol acyltransferase in Brassica napus results in changes in lipid metabolism and oil accumulation. Biochemical Journal, 479(6), https://doi.org/10.1042/bcj20220003

The regulation of lipid metabolism in oil seeds is still not fully understood and increasing our knowledge in this regard is of great economic, as well as intellectual, importance. Oilseed rape (Brassica napus) is a major global oil crop where increa... Read More about Overexpression of phospholipid: diacylglycerol acyltransferase in Brassica napus results in changes in lipid metabolism and oil accumulation.

Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism (2022)
Journal Article
Liao, P., Lechon, T., Romsdahl, T., Woodfield, H., Fenyk, S., Fawcett, T., Wallington, E., Bates, R. E., Chye, M.-L., Chapman, K. D., Harwood, J. L., & Scofield, S. (2022). Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism. Scientific Reports, 12(1), https://doi.org/10.1038/s41598-022-07387-x

Oilseed rape (Brassica napus) is an important crop that is cultivated for the oil (mainly triacylglycerol; TAG) it produces in its seeds. TAG synthesis is controlled mainly by key enzymes in the Kennedy pathway, such as glycerol 3-phosphate acyltrans... Read More about Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism.

Functional analyses of differentially expressed isoforms of the Arabidopsis inositol phosphorylceramide synthase (2010)
Journal Article
Mina, J., Okada, Y., Wansadhipathi-Kannangara, N., Pratt, S., Shams-Eldin, H., Schwarz, R., …Denny, P. (2010). Functional analyses of differentially expressed isoforms of the Arabidopsis inositol phosphorylceramide synthase. Plant Molecular Biology, 73(4-5), 399-407. https://doi.org/10.1007/s11103-010-9626-3

Sphingolipids are key components of eukaryotic plasma membranes that are involved in many functions, including the formation signal transduction complexes. In addition, these lipid species and their catabolites function as secondary signalling molecu... Read More about Functional analyses of differentially expressed isoforms of the Arabidopsis inositol phosphorylceramide synthase.

Tandem affinity purification tagging of fatty acid biosynthetic enzymesin Synechocystis sp PCC6803 and Arabidopsis thaliana (2006)
Journal Article
Brown, A. P., Affleck, V., Fawcett, T., & Slabas, A. R. (2006). Tandem affinity purification tagging of fatty acid biosynthetic enzymesin Synechocystis sp PCC6803 and Arabidopsis thaliana. Journal of Experimental Botany, 57(7), 1563-1571. https://doi.org/10.1093/jxb/erj150

De novo fatty acid synthesis in plants occurs primarily in the plastids and is catalysed by a type-II fatty acid synthase (FAS) in which separate enzymes catalyse sequential reactions. Genes encoding all of the plant FAS components have been identifi... Read More about Tandem affinity purification tagging of fatty acid biosynthetic enzymesin Synechocystis sp PCC6803 and Arabidopsis thaliana.

Squash glycerol-3-phosphate (1)-acyltransferase - Alteration of substrate selectivity and identification of arginine and lysineresidues important in catalytic activity (2002)
Journal Article
Slabas, A., Kroon, J., Scheirer, T., Gilroy, J., Hayman, M., Rice, D., …Simon, W. (2002). Squash glycerol-3-phosphate (1)-acyltransferase - Alteration of substrate selectivity and identification of arginine and lysineresidues important in catalytic activity. Journal of Biological Chemistry, 277(46), 43918-43923. https://doi.org/10.1074/jbc.m206429200

Glycerol-3-phosphate 1-acyltransferase is a soluble chloroplast enzyme involved in glycerol-lipid biosynthesis associated with chilling resistance in plants (1). Resistance is associated with higher selectivity for unsaturated acyl substrates over sa... Read More about Squash glycerol-3-phosphate (1)-acyltransferase - Alteration of substrate selectivity and identification of arginine and lysineresidues important in catalytic activity.

Fatty acid and lipid biosynthetic genes are expressed at constant molar ratios but different absolute levels during embryogenesis (2002)
Journal Article
O'Hara, P., Slabas, A., & Fawcett, T. (2002). Fatty acid and lipid biosynthetic genes are expressed at constant molar ratios but different absolute levels during embryogenesis. Plant Physiology, 129(1), 310-320. https://doi.org/10.1104/pp.010956

In plants, fatty acid and complex lipid synthesis requires the correct spatial and temporal activity of many gene products. Quantitative northern analysis showed that mRNA for the biotin carboxylase subunit of heteromeric acetyl-coenzyme A carboxylas... Read More about Fatty acid and lipid biosynthetic genes are expressed at constant molar ratios but different absolute levels during embryogenesis.

Analysis of the Structure, Substrate Specificity, and Mechanism of Squash Glycerol-3-Phosphate (1)-Acyltransferase. (2001)
Journal Article
Turnbull, A., Rafferty, J., Sedelnikova, S., Slabas, A., Scheirer, T., Kroon, J., …Rice, D. (2001). Analysis of the Structure, Substrate Specificity, and Mechanism of Squash Glycerol-3-Phosphate (1)-Acyltransferase. Structure, 9(5), 347-353. https://doi.org/10.1016/s0969-2126%2801%2900595-0

Background: Glycerol-3-phosphate (1)-acyltransferase(G3PAT) catalyzes the incorporation of an acyl group from either acyl-acyl carrier proteins (acylACPs) or acyl-CoAs into the sn-1 position of glycerol 3-phosphate to yield 1-acylglycerol-3-phosphate... Read More about Analysis of the Structure, Substrate Specificity, and Mechanism of Squash Glycerol-3-Phosphate (1)-Acyltransferase..