Outputs (5)

Insights into the antibacterial mechanism of action of chelating agents by selective deprivation of iron, manganese and zinc (2021)
Journal Article
Paterson, J. R., Beecroft, M. S., Mulla, R. S., Osman, D., Reeder, N. L., Caserta, J. A., Young, T. R., Pettigrew, C. A., Davies, G. E., Williams, J. G., & Sharples, G. J. (2022). Insights into the antibacterial mechanism of action of chelating agents by selective deprivation of iron, manganese and zinc. Applied and Environmental Microbiology, 88(2), Article e01641-21. https://doi.org/10.1128/aem.01641-21

Bacterial growth and proliferation can be restricted by limiting the availability of metal ions in their environment. Humans sequester iron, manganese and zinc to help prevent infection by pathogens, a system termed nutritional immunity. Commercially... Read More about Insights into the antibacterial mechanism of action of chelating agents by selective deprivation of iron, manganese and zinc.

Acquisition of ionic copper by the bacterial outer membrane protein OprC through a novel binding site (2021)
Journal Article
Bhamidimarri, S. P., Young, T. R., Shanmugam, M., Soderholm, S., Baslé, A., Bumann, D., & van den Berg, B. (2021). Acquisition of ionic copper by the bacterial outer membrane protein OprC through a novel binding site. PLoS Biology, 19(11), Article e3001446. https://doi.org/10.1371/journal.pbio.3001446

Copper, while toxic in excess, is an essential micronutrient in all kingdoms of life due to its essential role in the structure and function of many proteins. Proteins mediating ionic copper import have been characterised in detail for eukaryotes, bu... Read More about Acquisition of ionic copper by the bacterial outer membrane protein OprC through a novel binding site.

Protein metalation in biology (2021)
Journal Article
Foster, A. W., Young, T. R., Chivers, P. T., & Robinson, N. J. (2022). Protein metalation in biology. Current Opinion in Chemical Biology, 66, Article 102095. https://doi.org/10.1016/j.cbpa.2021.102095

Inorganic metals supplement the chemical repertoire of organic molecules, especially proteins. This requires the correct metals to associate with proteins at metalation. Protein mismetalation typically occurs when excesses of unbound metals compete f... Read More about Protein metalation in biology.

Principles and practice of determining metal–protein affinities (2021)
Journal Article
Young, T. R., & Xiao, Z. (2021). Principles and practice of determining metal–protein affinities. Biochemical Journal, 478(5), 1085-1116. https://doi.org/10.1042/bcj20200838

Metal ions play many critical roles in biology, as structural and catalytic cofactors, and as cell regulatory and signalling elements. The metal–protein affinity, expressed conveniently by the metal dissociation constant, KD, describes the thermodyna... Read More about Principles and practice of determining metal–protein affinities.

Calculating metalation in cells reveals CobW acquires Co(II) for vitamin B12 biosynthesis while related proteins prefer Zn(II) (2021)
Journal Article
Young, T., Martini, M., Foster, A., Glasfeld, A., Osman, D., Morton, R., Deery, E., Warren, M., & Robinson, N. (2021). Calculating metalation in cells reveals CobW acquires Co(II) for vitamin B12 biosynthesis while related proteins prefer Zn(II). Nature Communications, 12, Article 1195. https://doi.org/10.1038/s41467-021-21479-8

Protein metal-occupancy (metalation) in vivo has been elusive. To address this challenge, the available free energies of metals have recently been determined from the responses of metal sensors. Here, we use these free energy values to develop a meta... Read More about Calculating metalation in cells reveals CobW acquires Co(II) for vitamin B12 biosynthesis while related proteins prefer Zn(II).