Outputs (6)

Structure and rational engineering of the PglX methyltransferase and specificity factor for BREX phage defence (2024)
Journal Article
Went, S. C., Picton, D. M., Morgan, R. D., Nelson, A., Brady, A., Mariano, G., Dryden, D. T. F., Smith, D. L., Wenner, N., Hinton, J. C. D., & Blower, T. R. (2024). Structure and rational engineering of the PglX methyltransferase and specificity factor for BREX phage defence. Nature Communications, 15, Article 7236. https://doi.org/10.1038/s41467-024-51629-7

Bacteria have evolved a broad range of systems that provide defence against their viral predators, bacteriophages. Bacteriophage Exclusion (BREX) systems recognise and methylate 6 bp non-palindromic motifs within the host genome, and prevent replicat... Read More about Structure and rational engineering of the PglX methyltransferase and specificity factor for BREX phage defence.

Generation of tools for expression and purification of the phage-encoded Type I restriction enzyme inhibitor, Ocr (2024)
Journal Article
Alves, J., Dry, I., White, J. H., Dryden, D. T., & Lynskey, N. N. (2024). Generation of tools for expression and purification of the phage-encoded Type I restriction enzyme inhibitor, Ocr. Microbiology, 170(7), https://doi.org/10.1099/mic.0.001465

DNA manipulation is an essential tool in molecular microbiology research that is dependent on the ability of bacteria to take up and preserve foreign DNA by horizontal gene transfer. This process can be significantly impaired by the activity of bacte... Read More about Generation of tools for expression and purification of the phage-encoded Type I restriction enzyme inhibitor, Ocr.

The phage defence island of a multidrug resistant plasmid uses both BREX and type IV restriction for complementary protection from viruses (2021)
Journal Article
Picton, D., Luyten, Y., Morgan, R., Nelson, A., Smith, D., Dryden, D., Hinton, J., & Blower, T. (2021). The phage defence island of a multidrug resistant plasmid uses both BREX and type IV restriction for complementary protection from viruses. Nucleic Acids Research, 49(19), 11257-11273. https://doi.org/10.1093/nar/gkab906

Bacteria have evolved a multitude of systems to prevent invasion by bacteriophages and other mobile genetic elements. Comparative genomics suggests that genes encoding bacterial defence mechanisms are often clustered in “defence islands”, providing a... Read More about The phage defence island of a multidrug resistant plasmid uses both BREX and type IV restriction for complementary protection from viruses.

Structural basis of transcription inhibition by the DNA mimic protein Ocr of bacteriophage T7 (2020)
Journal Article
Ye, F., Kotta-Loizou, I., Jovanovic, M., Liu, X., Dryden, D. T., Buck, M., & Zhang, X. (2020). Structural basis of transcription inhibition by the DNA mimic protein Ocr of bacteriophage T7. eLife, 9, Article e52125. https://doi.org/10.7554/elife.52125

Bacteriophage T7 infects Escherichia coli and evades the host restriction/modification system. The Ocr protein of T7 was shown to exist as a dimer mimicking DNA and to bind to host restriction enzymes, thus preventing the degradation of the viral gen... Read More about Structural basis of transcription inhibition by the DNA mimic protein Ocr of bacteriophage T7.

A model for the evolution of prokaryotic DNA restriction-modification systems based upon the structural malleability of Type I restriction-modification enzymes (2018)
Journal Article
Bower, E., Cooper, L., Roberts, G., White, J., Luyten, Y., Morgan, R., & Dryden, D. (2018). A model for the evolution of prokaryotic DNA restriction-modification systems based upon the structural malleability of Type I restriction-modification enzymes. Nucleic Acids Research, 46(17), 9067-9080. https://doi.org/10.1093/nar/gky760

Restriction Modification (RM) systems prevent the invasion of foreign genetic material into bacterial cells by restriction and protect the host's genetic material by methylation. They are therefore important in maintaining the integrity of the host g... Read More about A model for the evolution of prokaryotic DNA restriction-modification systems based upon the structural malleability of Type I restriction-modification enzymes.

DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus (2017)
Journal Article
Cooper, L., Roberts, G., White, J., Luyten, Y., Bower, E., Morgan, R., …Dryden, D. (2017). DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus. Nucleic Acids Research, 45(6), 3395-3406. https://doi.org/10.1093/nar/gkx067

Staphylococcus aureus displays a clonal population structure in which horizontal gene transfer between different lineages is extremely rare. This is due, in part, to the presence of a Type I DNA restriction–modification (RM) system given the generic... Read More about DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus.