Outputs (5)

Bullseye Analysis: A Fluorescence Microscopy Technique to Detect Local Changes in Intracellular Reactive Oxygen Species (ROS) Production (2022)
Journal Article
Hughes, J. G., Chisholm, D. R., Whiting, A., Girkin, J. M., & Ambler, C. A. (2023). Bullseye Analysis: A Fluorescence Microscopy Technique to Detect Local Changes in Intracellular Reactive Oxygen Species (ROS) Production. Microscopy and Microanalysis, 29(2), 529-539. https://doi.org/10.1093/micmic/ozac040

Reactive oxygen species (ROS) are naturally produced compounds that play important roles in cell signaling, gene regulation, and biological defense, including involvement in the oxidative burst that is central to the anti-microbial actions of macroph... Read More about Bullseye Analysis: A Fluorescence Microscopy Technique to Detect Local Changes in Intracellular Reactive Oxygen Species (ROS) Production.

Cellular localisation of structurally diverse diphenylacetylene fluorophores (2020)
Journal Article
Chisholm, D. R., Hughes, J. G., Blacker, T. S., Humann, R., Adams, C., Callaghan, D., Pujol, A., Lembicz, N. K., Bain, A. J., Girkin, J. M., Ambler, C. A., & Whiting, A. (2020). Cellular localisation of structurally diverse diphenylacetylene fluorophores. Organic and Biomolecular Chemistry, 18(45), 9231-9245. https://doi.org/10.1039/d0ob01153c

Fluorescent probes are increasingly used as reporter molecules in a wide variety of biophysical experiments, but when designing new compounds it can often be difficult to anticipate the effect that changing chemical structure can have on cellular loc... Read More about Cellular localisation of structurally diverse diphenylacetylene fluorophores.

Detection and Time-Tracking Activation of a Photosensitiser on Live Single Colorectal Cancer Cells Using Raman Spectroscopy (2020)
Journal Article
Gala De Pablo, J., Chisholm, D., Ambler, C., Peyman, S., Whiting, A., & Evans, S. (2020). Detection and Time-Tracking Activation of a Photosensitiser on Live Single Colorectal Cancer Cells Using Raman Spectroscopy. Analyst, 145(17), 5878-5888. https://doi.org/10.1039/d0an01023e

Raman spectroscopy has been used to observe uptake, metabolism and response of single-cells to drugs. Photodynamic therapy is based on the use of light, a photosensitiser and oxygen to destroy tumour tissue. Here, we used single-cell Raman spectrosco... Read More about Detection and Time-Tracking Activation of a Photosensitiser on Live Single Colorectal Cancer Cells Using Raman Spectroscopy.

Fluorescent retinoic acid analogues as probes for biochemical and intracellular characterization of retinoid signalling pathways (2019)
Journal Article
Chisholm, D., Tomlinson, C., Zhou, G.-L., Holden, C., Affleck, V., Lamb, R., Newling, K., Ashton, P., Valentine, R., Redfern, C., Erostyak, J., Makkai, G., Ambler, C., Whiting, A., & Pohl, E. (2019). Fluorescent retinoic acid analogues as probes for biochemical and intracellular characterization of retinoid signalling pathways. ACS Chemical Biology, 14(3), 369-377. https://doi.org/10.1021/acschembio.8b00916

Retinoids, such as all-trans-retinoic acid (ATRA), are endogenous signalling molecules derived from Vitamin A that influ-ence a variety of cellular processes through mediation of transcription events in the cell nucleus. Due to these wide-ranging and... Read More about Fluorescent retinoic acid analogues as probes for biochemical and intracellular characterization of retinoid signalling pathways.

A novel fluorescence competition assay for retinoic acid binding proteins (2018)
Journal Article
Tomlinson, C. W., Chisholm, D. R., Valentine, R., Whiting, A., & Pohl, E. (2018). A novel fluorescence competition assay for retinoic acid binding proteins. ACS Medicinal Chemistry Letters, 9(12), 1297–1300-1300. https://doi.org/10.1021/acsmedchemlett.8b00420

Vitamin A derived retinoid compounds have multiple, powerful roles in the cellular growth and development cycle, and as a result have attracted significant attention from both academic and pharmaceutical research in developing and characterizing synt... Read More about A novel fluorescence competition assay for retinoic acid binding proteins.