Outputs (25)

NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity (2023)
Journal Article
Hawkins, T. J., Kopischke, M., Duckney, P. J., Rybak, K., Mentlak, D. A., Kroon, J. T. M., …Robatzek, S. (2023). NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity. Nature Communications, 14(1), Article 5848. https://doi.org/10.1038/s41467-023-41337-z

Members of the NETWORKED (NET) family are involved in actin-membrane interactions. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal cl... Read More about NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity.

STING nuclear partners contribute to innate immune signaling responses (2021)
Journal Article
Dixon, C. R., Malik, P., de las Heras, J. I., Saiz-Ros, N., de Lima Alves, F., Tingey, M., Gaunt, E., Richardson, A. C., Kelly, D. A., Goldberg, M. W., Towers, G. J., Yang, W., Rappsilber, J., Digard, P., & Schirmer, E. C. (2021). STING nuclear partners contribute to innate immune signaling responses. iScience, 24(9), https://doi.org/10.1016/j.isci.2021.103055

STimulator of INterferon Genes (STING) is an adaptor for cytoplasmic DNA sensing by cGAMP/cGAS that helps trigger innate immune responses (IIRs). Although STING is mostly localized in the ER, we find a separate inner nuclear membrane pool of STING th... Read More about STING nuclear partners contribute to innate immune signaling responses.

BBLN-1 is essential for intermediate filament organization and apical membrane morphology (2021)
Journal Article
Remmelzwaal, S., Geisler, F., Stucchi, R., van der Horst, S., Pasolli, M., Kroll, J. R., Jarosinska, O. D., Akhmanova, A., Richardson, C. A., Altelaar, M., Leube, R. E., Ramalho, J. J., & Boxem, M. (2021). BBLN-1 is essential for intermediate filament organization and apical membrane morphology. Current Biology, 31(11), 2334-2346. https://doi.org/10.1016/j.cub.2021.03.069

Epithelial tubes are essential components of metazoan organ systems that control the flow of fluids and the exchange of materials between body compartments and the outside environment. The size and shape of the central lumen confer important characte... Read More about BBLN-1 is essential for intermediate filament organization and apical membrane morphology.

Intestinal intermediate filament polypeptides in C. elegans: Common and isotype-specific contributions to intestinal ultrastructure and function (2020)
Journal Article
Geisler, F., Coch, R. A., Richardson, C., Goldberg, M., Bevilacqua, C., Prevede, R., & Leube, R. E. (2020). Intestinal intermediate filament polypeptides in C. elegans: Common and isotype-specific contributions to intestinal ultrastructure and function. Scientific Reports, 10, Article 3142. https://doi.org/10.1038/s41598-020-59791-w

The abundance and diversity of intermediate filaments (IFs) in the C. elegans intestine indicate important contributions to intestinal function and organismal wellbeing. Fluorescent IF reporters localize below the actin-rich brush border and are high... Read More about Intestinal intermediate filament polypeptides in C. elegans: Common and isotype-specific contributions to intestinal ultrastructure and function.

Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery (2019)
Journal Article
Wang, P., Pleskot, R., Zang, J., Winkler, J., Wang, J., Yperman, K., Zhang, T., Wang, K., Gong, J., Guan, Y., Richardson, C., Duckney, P., Vandorpe, M., Mylle, E., Fiserova, J., Van Damme, D., & Hussey, P. J. (2019). Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery. Nature Communications, 10(1), Article 5132. https://doi.org/10.1038/s41467-019-12782-6

The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these prote... Read More about Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery.

Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication (2019)
Journal Article
Saiz-Ros, N., Czapiewski, R., Epifano, I., Stevenson, A., Swanson, S., Dixon, C., Zamora, D., McElwee, M., Vijayakrishnan, S., Richardson, C., Dong, L., Kelly, D., Pytowski, L., Goldberg, M., Florens, L., Graham, S., & Schirmer, E. (2019). Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication. Cells, 8(2), Article 120. https://doi.org/10.3390/cells8020120

The primary envelopment/de-envelopment of Herpes viruses during nuclear exit is poorly understood. In Herpes simplex virus type-1 (HSV-1), proteins pUL31 and pUL34 are critical, while pUS3 and some others contribute; however, efficient membrane fusio... Read More about Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication.

The intestinal intermediate filament network responds to and protects against microbial insults and toxins (2019)
Journal Article
Geisler, F., Coch, R. A., Richardson, C., Goldberg, M., Denecke, B., Bossinger, O., & Leube, R. E. (2019). The intestinal intermediate filament network responds to and protects against microbial insults and toxins. Development, 146(2), Article dev.169482. https://doi.org/10.1242/dev.169482

The enrichment of intermediate filaments in the apical cytoplasm of intestinal cells is evolutionary conserved forming a sheath that is anchored to apical junctions and positioned below the microvillar brush border suggestive of a protective intracel... Read More about The intestinal intermediate filament network responds to and protects against microbial insults and toxins.

Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution (2018)
Journal Article
Reipert, S., Goldammer, H., Richardson, C., Goldberg, M. W., Hawkins, T. J., Hollergschwandtner, E., Kaufmann, W. A., Antreich, S., & Stierhof, Y.-D. (2018). Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution. Journal of Histochemistry & Cytochemistry, 66(12), 903-921. https://doi.org/10.1369/0022155418786698

For ultrafast fixation of biological samples to avoid artifacts, high-pressure freezing (HPF) followed by freeze substitution (FS) is preferred over chemical fixation at room temperature. After HPF, samples are maintained at low temperature during de... Read More about Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution.

Characterization of Proteins Localized to Plant ER-PM Contact Sites (2017)
Book Chapter
Wang, P., Hawes, C., Richardson, C., & Hussey, P. J. (2018). Characterization of Proteins Localized to Plant ER-PM Contact Sites. In C. Hawes, & V. Kriechbaumer (Eds.), The Plant Endoplasmic Reticulum (23-31). Humana Press. https://doi.org/10.1007/978-1-4939-7389-7_3

Like in most eukaryotic cells, the plant endoplasmic reticulum (ER) network is physically linked to the plasma membrane (PM), forming ER-PM contact sites (EPCS). The protein complex required for maintaining the EPCS is composed of ER integral membran... Read More about Characterization of Proteins Localized to Plant ER-PM Contact Sites.

A rim-and-spoke hypothesis to explain the biomechanical roles for cytoplasmic intermediate filament networks (2017)
Journal Article
Quinlan, R., Schwartz, N., Windoffer, R., Richardson, C., Hawkins, T., Broussard, J., …Leube, R. (2017). A rim-and-spoke hypothesis to explain the biomechanical roles for cytoplasmic intermediate filament networks. Journal of Cell Science, 130(20), 3437-3445. https://doi.org/10.1242/jcs.202168

Textbook images of keratin intermediate filament (IF) networks in epithelial cells and the functional compromization of the epidermis by keratin mutations promulgate a mechanical role for this important cytoskeletal component. In stratified epithelia... Read More about A rim-and-spoke hypothesis to explain the biomechanical roles for cytoplasmic intermediate filament networks.