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Nucleotidyltransferase toxin MenT extends aminoacyl acceptor ends of serine tRNAs to control Mycobacterium tuberculosis growth (2024)
Journal Article
Xu, X., Barriot, R., Voisin, B., Arrowsmith, T. J., Usher, B., Gutierrez, C., Han, X., Pagès, C., Redder, P., Blower, T. R., Neyrolles, O., & Genevaux, P. (2024). Nucleotidyltransferase toxin MenT extends aminoacyl acceptor ends of serine tRNAs to control Mycobacterium tuberculosis growth. Nature Communications, 15(1), Article 9596. https://doi.org/10.1038/s41467-024-53931-w

Toxins of toxin-antitoxin systems use diverse mechanisms to inhibit bacterial growth. In this study, we characterize the translation inhibitor toxin MenT3 of Mycobacterium tuberculosis, the bacterium responsible for tuberculosis in humans. We show th... Read More about Nucleotidyltransferase toxin MenT extends aminoacyl acceptor ends of serine tRNAs to control Mycobacterium tuberculosis growth.

Inducible auto-phosphorylation regulates a widespread family of nucleotidyltransferase toxins (2024)
Journal Article
Arrowsmith, T. J., Xu, X., Xu, S., Usher, B., Stokes, P., Guest, M., Bronowska, A. K., Genevaux, P., & Blower, T. R. (2024). Inducible auto-phosphorylation regulates a widespread family of nucleotidyltransferase toxins. Nature Communications, 15(1), Article 7719. https://doi.org/10.1038/s41467-024-51934-1

Nucleotidyltransferases (NTases) control diverse physiological processes, including RNA modification, DNA replication and repair, and antibiotic resistance. The Mycobacterium tuberculosis NTase toxin family, MenT, modifies tRNAs to block translation.... Read More about Inducible auto-phosphorylation regulates a widespread family of nucleotidyltransferase toxins.

Phage anti-CRISPR control by an RNA- and DNA-binding helix–turn–helix protein (2024)
Journal Article
Birkholz, N., Kamata, K., Feussner, M., Wilkinson, M. E., Cuba Samaniego, C., Migur, A., Kimanius, D., Ceelen, M., Went, S. C., Usher, B., Blower, T. R., Brown, C. M., Beisel, C. L., Weinberg, Z., Fagerlund, R. D., Jackson, S. A., & Fineran, P. C. (2024). Phage anti-CRISPR control by an RNA- and DNA-binding helix–turn–helix protein. Nature, 631(8021), 670-677. https://doi.org/10.1038/s41586-024-07644-1

In all organisms, regulation of gene expression must be adjusted to meet cellular requirements and frequently involves helix-turn-helix (HTH) domain proteins . For instance, in the arms race between bacteria and bacteriophages, rapid expression of ph... Read More about Phage anti-CRISPR control by an RNA- and DNA-binding helix–turn–helix protein.

MenT nucleotidyltransferase toxins extend tRNA acceptor stems and can be inhibited by asymmetrical antitoxin binding (2023)
Journal Article
Xu, X., Usher, B., Gutierrez, C., Barriot, R., Arrowsmith, T. J., Han, X., …Genevaux, P. (2023). MenT nucleotidyltransferase toxins extend tRNA acceptor stems and can be inhibited by asymmetrical antitoxin binding. Nature Communications, 14, Article 4644. https://doi.org/10.1038/s41467-023-40264-3

Mycobacterium tuberculosis, the bacterium responsible for human tuberculosis, has a genome encoding a remarkably high number of toxin-antitoxin systems of largely unknown function. We have recently shown that the M. tuberculosis genome encodes four o... Read More about MenT nucleotidyltransferase toxins extend tRNA acceptor stems and can be inhibited by asymmetrical antitoxin binding.